CD44s expression correlated with the International Neuroblastoma Pathology Classification (Shimada system) for neuroblastic tumours

M. J Julia Munchar, N. A. Sharifah, A. Rahman A. Jamal, L. M. Looi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Aim: CD44 is a cell adhesion molecule that plays an important role in the cascade of metastasis and progression of human malignant tumours. A large family of variants or isoforms, generated by alternative splicing of a single gene, has been reported to be involved in the malignant process by conferring metastatic potential to non-metastatic cells. The objective of this study was to compare the expression of CD44 standard molecule with the International Neuroblastoma Pathology Classification (INPC) for neuroblastic tumours, a histological grading system based on the Shimada system for predicting the clinical outcome in neuroblastic tumours. Methods: Paraffin blocks of primary neuroblastic tumour were graded according to the INPC system into favourable and unfavourable histological types. Tumour tissues were then analysed with immunostaining using monoclonal antibodies against the CD44 epitope. Results: In this retrospective study, 32 cases of primary neuroblastic tumour were collected. Based on the INPC system, 13 cases (40.6%) had a favourable histology while 19 cases (59.4%) were of unfavourable histology. CD44 standard (CD44s) expression was detected in 69.2% of neuroblastic tumours with favourable histological type while 89.5% of tumours with unfavourable histological type did not express CD44s staining. Statistically, there was significant correlation between morphological grading of neuroblastic tumours based on the INPC and the presence of CD44 staining (Fisher's exact test, P<0.05). Conclusion: This study shows that there is a significant correlation between CD44s expression and tumour histology based on the INPC in which CD44s non-expression is correlated with an unfavourable histological type and the expression of CD44s with a favourable type. Therefore, the analysis of CD44s expression should be recommended as an additional biological marker in the initial routine staging of the disease.

Original languageEnglish
Pages (from-to)125-129
Number of pages5
JournalPathology
Volume35
Issue number2
DOIs
Publication statusPublished - Apr 2003

Fingerprint

Neuroblastoma
Pathology
Neoplasms
Histology
Staining and Labeling
Neoplasm Grading
Alternative Splicing
Cell Adhesion Molecules
Paraffin
Epitopes
Protein Isoforms
Retrospective Studies
Biomarkers
Monoclonal Antibodies
Neoplasm Metastasis
Genes

Keywords

  • CD44
  • Neuroblastoma
  • Shimada's histological classification

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

CD44s expression correlated with the International Neuroblastoma Pathology Classification (Shimada system) for neuroblastic tumours. / Munchar, M. J Julia; Sharifah, N. A.; A. Jamal, A. Rahman; Looi, L. M.

In: Pathology, Vol. 35, No. 2, 04.2003, p. 125-129.

Research output: Contribution to journalArticle

@article{392c48a66dc94053820ceda12f8f65ab,
title = "CD44s expression correlated with the International Neuroblastoma Pathology Classification (Shimada system) for neuroblastic tumours",
abstract = "Aim: CD44 is a cell adhesion molecule that plays an important role in the cascade of metastasis and progression of human malignant tumours. A large family of variants or isoforms, generated by alternative splicing of a single gene, has been reported to be involved in the malignant process by conferring metastatic potential to non-metastatic cells. The objective of this study was to compare the expression of CD44 standard molecule with the International Neuroblastoma Pathology Classification (INPC) for neuroblastic tumours, a histological grading system based on the Shimada system for predicting the clinical outcome in neuroblastic tumours. Methods: Paraffin blocks of primary neuroblastic tumour were graded according to the INPC system into favourable and unfavourable histological types. Tumour tissues were then analysed with immunostaining using monoclonal antibodies against the CD44 epitope. Results: In this retrospective study, 32 cases of primary neuroblastic tumour were collected. Based on the INPC system, 13 cases (40.6{\%}) had a favourable histology while 19 cases (59.4{\%}) were of unfavourable histology. CD44 standard (CD44s) expression was detected in 69.2{\%} of neuroblastic tumours with favourable histological type while 89.5{\%} of tumours with unfavourable histological type did not express CD44s staining. Statistically, there was significant correlation between morphological grading of neuroblastic tumours based on the INPC and the presence of CD44 staining (Fisher's exact test, P<0.05). Conclusion: This study shows that there is a significant correlation between CD44s expression and tumour histology based on the INPC in which CD44s non-expression is correlated with an unfavourable histological type and the expression of CD44s with a favourable type. Therefore, the analysis of CD44s expression should be recommended as an additional biological marker in the initial routine staging of the disease.",
keywords = "CD44, Neuroblastoma, Shimada's histological classification",
author = "Munchar, {M. J Julia} and Sharifah, {N. A.} and {A. Jamal}, {A. Rahman} and Looi, {L. M.}",
year = "2003",
month = "4",
doi = "10.1080/00313020307573",
language = "English",
volume = "35",
pages = "125--129",
journal = "Pathology",
issn = "0031-3025",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - CD44s expression correlated with the International Neuroblastoma Pathology Classification (Shimada system) for neuroblastic tumours

AU - Munchar, M. J Julia

AU - Sharifah, N. A.

AU - A. Jamal, A. Rahman

AU - Looi, L. M.

PY - 2003/4

Y1 - 2003/4

N2 - Aim: CD44 is a cell adhesion molecule that plays an important role in the cascade of metastasis and progression of human malignant tumours. A large family of variants or isoforms, generated by alternative splicing of a single gene, has been reported to be involved in the malignant process by conferring metastatic potential to non-metastatic cells. The objective of this study was to compare the expression of CD44 standard molecule with the International Neuroblastoma Pathology Classification (INPC) for neuroblastic tumours, a histological grading system based on the Shimada system for predicting the clinical outcome in neuroblastic tumours. Methods: Paraffin blocks of primary neuroblastic tumour were graded according to the INPC system into favourable and unfavourable histological types. Tumour tissues were then analysed with immunostaining using monoclonal antibodies against the CD44 epitope. Results: In this retrospective study, 32 cases of primary neuroblastic tumour were collected. Based on the INPC system, 13 cases (40.6%) had a favourable histology while 19 cases (59.4%) were of unfavourable histology. CD44 standard (CD44s) expression was detected in 69.2% of neuroblastic tumours with favourable histological type while 89.5% of tumours with unfavourable histological type did not express CD44s staining. Statistically, there was significant correlation between morphological grading of neuroblastic tumours based on the INPC and the presence of CD44 staining (Fisher's exact test, P<0.05). Conclusion: This study shows that there is a significant correlation between CD44s expression and tumour histology based on the INPC in which CD44s non-expression is correlated with an unfavourable histological type and the expression of CD44s with a favourable type. Therefore, the analysis of CD44s expression should be recommended as an additional biological marker in the initial routine staging of the disease.

AB - Aim: CD44 is a cell adhesion molecule that plays an important role in the cascade of metastasis and progression of human malignant tumours. A large family of variants or isoforms, generated by alternative splicing of a single gene, has been reported to be involved in the malignant process by conferring metastatic potential to non-metastatic cells. The objective of this study was to compare the expression of CD44 standard molecule with the International Neuroblastoma Pathology Classification (INPC) for neuroblastic tumours, a histological grading system based on the Shimada system for predicting the clinical outcome in neuroblastic tumours. Methods: Paraffin blocks of primary neuroblastic tumour were graded according to the INPC system into favourable and unfavourable histological types. Tumour tissues were then analysed with immunostaining using monoclonal antibodies against the CD44 epitope. Results: In this retrospective study, 32 cases of primary neuroblastic tumour were collected. Based on the INPC system, 13 cases (40.6%) had a favourable histology while 19 cases (59.4%) were of unfavourable histology. CD44 standard (CD44s) expression was detected in 69.2% of neuroblastic tumours with favourable histological type while 89.5% of tumours with unfavourable histological type did not express CD44s staining. Statistically, there was significant correlation between morphological grading of neuroblastic tumours based on the INPC and the presence of CD44 staining (Fisher's exact test, P<0.05). Conclusion: This study shows that there is a significant correlation between CD44s expression and tumour histology based on the INPC in which CD44s non-expression is correlated with an unfavourable histological type and the expression of CD44s with a favourable type. Therefore, the analysis of CD44s expression should be recommended as an additional biological marker in the initial routine staging of the disease.

KW - CD44

KW - Neuroblastoma

KW - Shimada's histological classification

UR - http://www.scopus.com/inward/record.url?scp=0037613674&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037613674&partnerID=8YFLogxK

U2 - 10.1080/00313020307573

DO - 10.1080/00313020307573

M3 - Article

C2 - 12745459

AN - SCOPUS:0037613674

VL - 35

SP - 125

EP - 129

JO - Pathology

JF - Pathology

SN - 0031-3025

IS - 2

ER -