Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells

Salmaan H. Inayat-Hussain, Annuar B. Osman, Laily B. Din, Abdul M. Ali, Roger T. Snowden, Marion MacFarlane, Kelvin Cain

    Research output: Contribution to journalArticle

    68 Citations (Scopus)

    Abstract

    Goniothalamin, a plant styrylpyrone derivative isolated from Goniothalamus andersonii, induced apoptosis in Jurkat T-cells as assessed by the externalisation of phosphatidylserine. Immunoblotting showed processing of caspases-3 and -7 with the appearance of their catalytically active large subunits of 17 and 19 kDa, respectively. Activation of these caspases was further evidenced by detection of poly(ADP-ribose) polymerase cleavage (PARP). Pre-treatment with the caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp fluoromethyl ketone (Z-VAD.FMK) blocked apoptosis and the resultant cleavage of these caspases and PARP. Our results demonstrate that activation of at least two effector caspases is a key feature of goniothalamin-induced apoptosis in Jurkat T-cells. Copyright (C) 1999 Federation of European Biochemical Societies.

    Original languageEnglish
    Pages (from-to)379-383
    Number of pages5
    JournalFEBS Letters
    Volume456
    Issue number3
    DOIs
    Publication statusPublished - 13 Aug 1999

    Fingerprint

    Caspase 7
    Jurkat Cells
    T-cells
    Caspase 3
    Apoptosis
    Caspases
    T-Lymphocytes
    Goniothalamus
    Chemical activation
    Effector Caspases
    Caspase Inhibitors
    Poly(ADP-ribose) Polymerases
    Phosphatidylserines
    Ketones
    Immunoblotting
    Derivatives
    Processing
    goniothalamin

    Keywords

    • Apoptosis
    • Caspase-3
    • Caspase-7
    • Goniothalamin
    • Jurkat T-cell

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

    Cite this

    Inayat-Hussain, S. H., Osman, A. B., Din, L. B., Ali, A. M., Snowden, R. T., MacFarlane, M., & Cain, K. (1999). Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells. FEBS Letters, 456(3), 379-383. https://doi.org/10.1016/S0014-5793(99)00984-9

    Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells. / Inayat-Hussain, Salmaan H.; Osman, Annuar B.; Din, Laily B.; Ali, Abdul M.; Snowden, Roger T.; MacFarlane, Marion; Cain, Kelvin.

    In: FEBS Letters, Vol. 456, No. 3, 13.08.1999, p. 379-383.

    Research output: Contribution to journalArticle

    Inayat-Hussain, SH, Osman, AB, Din, LB, Ali, AM, Snowden, RT, MacFarlane, M & Cain, K 1999, 'Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells', FEBS Letters, vol. 456, no. 3, pp. 379-383. https://doi.org/10.1016/S0014-5793(99)00984-9
    Inayat-Hussain SH, Osman AB, Din LB, Ali AM, Snowden RT, MacFarlane M et al. Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells. FEBS Letters. 1999 Aug 13;456(3):379-383. https://doi.org/10.1016/S0014-5793(99)00984-9
    Inayat-Hussain, Salmaan H. ; Osman, Annuar B. ; Din, Laily B. ; Ali, Abdul M. ; Snowden, Roger T. ; MacFarlane, Marion ; Cain, Kelvin. / Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells. In: FEBS Letters. 1999 ; Vol. 456, No. 3. pp. 379-383.
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    AU - Din, Laily B.

    AU - Ali, Abdul M.

    AU - Snowden, Roger T.

    AU - MacFarlane, Marion

    AU - Cain, Kelvin

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