cagA gene variants in Malaysian Helicobacter pylori strains isolated from patients of different ethnic groups

Mohamed Ramelah, Ahmad Aminuddin, Alfizah Hanafiah, Mohd Rose Isa, Ali Yaakob Jasmi, Joo Tan Huck, A. Rahman A. Jamal, Mohd Rizal Abdul Manaf, M. Zawawi Mazlam

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Helicobacter pylori infection of a distinct subtype of cagA may lead to different pathological manifestation. The aim of this study is to determine the presence of cagA gene and its variants in H. pylori infection among different ethnic groups and its effect on gastroduodenal diseases. Overall detection of cagA among the 205 clinical isolates of H. pylori was 94%. Variations in size of the 3′ region of cagA gene were examined among 192 Malaysian H. pylori cagA-positive strains. Results showed that three cagA variants differing in fragment length of PCR products were detected and designated as type A (621-651 bp), type B (732-735 bp) and type C (525 bp). Although there was no association between any of the cagA subtypes with peptic ulcer disease (p > 0.05), an association between cagA subtypes with a specific ethnic group was observed. Specific-cagA subtype A strains were predominantly isolated from Chinese compared to Malays and Indians (p < 0.0005), and cagA subtype B strains were predominantly isolated from Malays and Indians compared to Chinese (p < 0.05). The cagA type A strains of H. pylori is commonly found in the Chinese patients who have a higher risk of peptic ulcer disease, thus indicating that it could be used as an important clinical biomarker for a more severe infection.

Original languageEnglish
Pages (from-to)239-242
Number of pages4
JournalFEMS Immunology and Medical Microbiology
Volume44
Issue number2
DOIs
Publication statusPublished - 1 May 2005

Fingerprint

Ethnic Groups
Helicobacter pylori
Genes
Helicobacter Infections
Peptic Ulcer
Biomarkers
Polymerase Chain Reaction
Infection

Keywords

  • cagA
  • Ethnicity
  • Genotype
  • Helicobacter pylori
  • Peptic ulcer

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Infectious Diseases

Cite this

cagA gene variants in Malaysian Helicobacter pylori strains isolated from patients of different ethnic groups. / Ramelah, Mohamed; Aminuddin, Ahmad; Hanafiah, Alfizah; Isa, Mohd Rose; Jasmi, Ali Yaakob; Huck, Joo Tan; A. Jamal, A. Rahman; Abdul Manaf, Mohd Rizal; Mazlam, M. Zawawi.

In: FEMS Immunology and Medical Microbiology, Vol. 44, No. 2, 01.05.2005, p. 239-242.

Research output: Contribution to journalArticle

Ramelah, Mohamed ; Aminuddin, Ahmad ; Hanafiah, Alfizah ; Isa, Mohd Rose ; Jasmi, Ali Yaakob ; Huck, Joo Tan ; A. Jamal, A. Rahman ; Abdul Manaf, Mohd Rizal ; Mazlam, M. Zawawi. / cagA gene variants in Malaysian Helicobacter pylori strains isolated from patients of different ethnic groups. In: FEMS Immunology and Medical Microbiology. 2005 ; Vol. 44, No. 2. pp. 239-242.
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