Burkholderia pseudomallei transcriptional adaptation in macrophages

Sylvia Chieng, Laura Carreto, Sheila Nathan

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: Burkholderia pseudomallei is a facultative intracellular pathogen of phagocytic and non-phagocytic cells. How the bacterium interacts with host macrophage cells is still not well understood and is critical to appreciate the strategies used by this bacterium to survive and how intracellular survival leads to disease manifestation.Results: Here we report the expression profile of intracellular B. pseudomallei following infection of human macrophage-like U937 cells. During intracellular growth over the 6 h infection period, approximately 22 % of the B. pseudomallei genome showed significant transcriptional adaptation. B. pseudomallei adapted rapidly to the intracellular environment by down-regulating numerous genes involved in metabolism, cell envelope, motility, replication, amino acid and ion transport system and regulatory function pathways. Reduced expression in catabolic and housekeeping genes suggested lower energy requirement and growth arrest during macrophage infection, while expression of genes encoding anaerobic metabolism functions were up regulated. However, whilst the type VI secretion system was up regulated, expression of many known virulence factors was not significantly modulated over the 6hours of infection.Conclusions: The transcriptome profile described here provides the first comprehensive view of how B. pseudomallei survives within host cells and will help identify potential virulence factors and proteins that are important for the survival and growth of B. pseudomallei within human cells.

Original languageEnglish
Article number328
JournalBMC Genomics
Volume13
Issue number1
DOIs
Publication statusPublished - 23 Jul 2012

Fingerprint

Burkholderia pseudomallei
Macrophages
Virulence Factors
Infection
Growth
Anaerobiosis
Bacteria
Amino Acid Transport Systems
U937 Cells
Survival
Essential Genes
Ion Transport
Transcriptome
Cell Movement
Genome
Gene Expression
Genes

Keywords

  • Burkholderia pseudomallei
  • Macrophage
  • Transcriptome analysis

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

Burkholderia pseudomallei transcriptional adaptation in macrophages. / Chieng, Sylvia; Carreto, Laura; Nathan, Sheila.

In: BMC Genomics, Vol. 13, No. 1, 328, 23.07.2012.

Research output: Contribution to journalArticle

Chieng, Sylvia ; Carreto, Laura ; Nathan, Sheila. / Burkholderia pseudomallei transcriptional adaptation in macrophages. In: BMC Genomics. 2012 ; Vol. 13, No. 1.
@article{90035a3a42164bfabcbf68d12a9fa562,
title = "Burkholderia pseudomallei transcriptional adaptation in macrophages",
abstract = "Background: Burkholderia pseudomallei is a facultative intracellular pathogen of phagocytic and non-phagocytic cells. How the bacterium interacts with host macrophage cells is still not well understood and is critical to appreciate the strategies used by this bacterium to survive and how intracellular survival leads to disease manifestation.Results: Here we report the expression profile of intracellular B. pseudomallei following infection of human macrophage-like U937 cells. During intracellular growth over the 6 h infection period, approximately 22 {\%} of the B. pseudomallei genome showed significant transcriptional adaptation. B. pseudomallei adapted rapidly to the intracellular environment by down-regulating numerous genes involved in metabolism, cell envelope, motility, replication, amino acid and ion transport system and regulatory function pathways. Reduced expression in catabolic and housekeeping genes suggested lower energy requirement and growth arrest during macrophage infection, while expression of genes encoding anaerobic metabolism functions were up regulated. However, whilst the type VI secretion system was up regulated, expression of many known virulence factors was not significantly modulated over the 6hours of infection.Conclusions: The transcriptome profile described here provides the first comprehensive view of how B. pseudomallei survives within host cells and will help identify potential virulence factors and proteins that are important for the survival and growth of B. pseudomallei within human cells.",
keywords = "Burkholderia pseudomallei, Macrophage, Transcriptome analysis",
author = "Sylvia Chieng and Laura Carreto and Sheila Nathan",
year = "2012",
month = "7",
day = "23",
doi = "10.1186/1471-2164-13-328",
language = "English",
volume = "13",
journal = "BMC Genomics",
issn = "1471-2164",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Burkholderia pseudomallei transcriptional adaptation in macrophages

AU - Chieng, Sylvia

AU - Carreto, Laura

AU - Nathan, Sheila

PY - 2012/7/23

Y1 - 2012/7/23

N2 - Background: Burkholderia pseudomallei is a facultative intracellular pathogen of phagocytic and non-phagocytic cells. How the bacterium interacts with host macrophage cells is still not well understood and is critical to appreciate the strategies used by this bacterium to survive and how intracellular survival leads to disease manifestation.Results: Here we report the expression profile of intracellular B. pseudomallei following infection of human macrophage-like U937 cells. During intracellular growth over the 6 h infection period, approximately 22 % of the B. pseudomallei genome showed significant transcriptional adaptation. B. pseudomallei adapted rapidly to the intracellular environment by down-regulating numerous genes involved in metabolism, cell envelope, motility, replication, amino acid and ion transport system and regulatory function pathways. Reduced expression in catabolic and housekeeping genes suggested lower energy requirement and growth arrest during macrophage infection, while expression of genes encoding anaerobic metabolism functions were up regulated. However, whilst the type VI secretion system was up regulated, expression of many known virulence factors was not significantly modulated over the 6hours of infection.Conclusions: The transcriptome profile described here provides the first comprehensive view of how B. pseudomallei survives within host cells and will help identify potential virulence factors and proteins that are important for the survival and growth of B. pseudomallei within human cells.

AB - Background: Burkholderia pseudomallei is a facultative intracellular pathogen of phagocytic and non-phagocytic cells. How the bacterium interacts with host macrophage cells is still not well understood and is critical to appreciate the strategies used by this bacterium to survive and how intracellular survival leads to disease manifestation.Results: Here we report the expression profile of intracellular B. pseudomallei following infection of human macrophage-like U937 cells. During intracellular growth over the 6 h infection period, approximately 22 % of the B. pseudomallei genome showed significant transcriptional adaptation. B. pseudomallei adapted rapidly to the intracellular environment by down-regulating numerous genes involved in metabolism, cell envelope, motility, replication, amino acid and ion transport system and regulatory function pathways. Reduced expression in catabolic and housekeeping genes suggested lower energy requirement and growth arrest during macrophage infection, while expression of genes encoding anaerobic metabolism functions were up regulated. However, whilst the type VI secretion system was up regulated, expression of many known virulence factors was not significantly modulated over the 6hours of infection.Conclusions: The transcriptome profile described here provides the first comprehensive view of how B. pseudomallei survives within host cells and will help identify potential virulence factors and proteins that are important for the survival and growth of B. pseudomallei within human cells.

KW - Burkholderia pseudomallei

KW - Macrophage

KW - Transcriptome analysis

UR - http://www.scopus.com/inward/record.url?scp=84865116538&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865116538&partnerID=8YFLogxK

U2 - 10.1186/1471-2164-13-328

DO - 10.1186/1471-2164-13-328

M3 - Article

C2 - 22823543

AN - SCOPUS:84865116538

VL - 13

JO - BMC Genomics

JF - BMC Genomics

SN - 1471-2164

IS - 1

M1 - 328

ER -