Burkholderia pseudomallei animal and human isolates from Malaysia exhibit different phenotypic characteristics

Song Hua Lee, Chan Eng Chong, Boon San Lim, San Jiun Chai, Kin Kit Sam, Rahmah Mohamed, Sheila Nathan

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Burkholderia pseudomallei is a Gram-negative saprophytic soil bacterium, which is the etiologic agent of melioidosis, a severe and fatal infectious disease occurring in human and animals. Distinct clinical and animal isolates have been shown to exhibit differences in phenotypic trait such as growth rate, colony morphology, antimicrobial resistance, and virulence. This study was carried out to gain insight into the intrinsic differences between 4 clinical and 6 animal B. pseudomallei isolates from Malaysia. The 16S rRNA-encoding genes from these 10 isolates of B. pseudomallei were sequenced to confirm the identity of these isolates along with the avirulent Burkholderia thailandensis. The nucleotide sequences indicated that the 16S rRNA-encoding genes among the 10 B. pseudomallei isolates were identical to each other. However, the nucleotide sequence differences in the 16S rRNA-encoding genes appeared to be B. pseudomallei and B. thailandensis specific. The growth rate of all B. pseudomallei isolates was determined by generating growth curves at 37 °C for 72 h. The isolates were found to differ in growth rates with doubling time varying from 1.5 to 2.3 h. In addition, the B. pseudomallei isolates exhibited considerable variation in colony morphology when grown on Ashdown media, brain-heart infusion agar, and Luria-Bertani agar over 9 days of observation. Antimicrobial susceptibility tests indicated that 80% of the isolates examined were AmpR CbR KnR GmR ChlS TeS. Virulence of the B. pseudomallei clinical and animal isolates was evaluated in B. pseudomallei-susceptible BALB/c mice. Most of the clinical isolates were highly virulent. However, virulence did not correlate with isolate origin since 2 of the animal isolates were also highly virulent.

Original languageEnglish
Pages (from-to)263-270
Number of pages8
JournalDiagnostic Microbiology and Infectious Disease
Volume58
Issue number3
DOIs
Publication statusPublished - Jul 2007

Fingerprint

Burkholderia pseudomallei
Malaysia
rRNA Genes
Virulence
Growth
Agar
Melioidosis
Burkholderia
Communicable Diseases
Soil
Observation
Bacteria

Keywords

  • Burkholderia pseudomallei
  • Melioidosis
  • Phenotypic

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Virology
  • Parasitology
  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology

Cite this

Burkholderia pseudomallei animal and human isolates from Malaysia exhibit different phenotypic characteristics. / Lee, Song Hua; Chong, Chan Eng; Lim, Boon San; Chai, San Jiun; Sam, Kin Kit; Mohamed, Rahmah; Nathan, Sheila.

In: Diagnostic Microbiology and Infectious Disease, Vol. 58, No. 3, 07.2007, p. 263-270.

Research output: Contribution to journalArticle

Lee, Song Hua ; Chong, Chan Eng ; Lim, Boon San ; Chai, San Jiun ; Sam, Kin Kit ; Mohamed, Rahmah ; Nathan, Sheila. / Burkholderia pseudomallei animal and human isolates from Malaysia exhibit different phenotypic characteristics. In: Diagnostic Microbiology and Infectious Disease. 2007 ; Vol. 58, No. 3. pp. 263-270.
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