Bull spermatozoa motility: Optimization of coenzyme Q10 and alpha-lipoic acid concentration

Siti Fatimah Ibrahim, Jaffar Farah Hanan Fathihah, Khairul Osman, Syarifah Faezah Syed Mohamed, Chew Fang Nang, Nur Hilwani Ismail, Mohd Iswadi Ismail

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

INTRODUCTION: Coenzyme Q10 (CoQ10) and alpha-lipoic acid (ALA) are antioxidants that play a role in ATP production and breakdown of free radicals. OBJECTIVE: The aim of this study was to optimize the concentration of CoQ10 and ALA based on motility pattern of spermatozoa. METHODS: The pooled bull spermatozoa were initially sub-fracted via electrophoretic separation which was performed at 20V for every two, four and six minutes. The sub-fractions were then further incubated with 0, 0.375, 0.75, 1.5, 3.0 and 6.0 μM of CoQ10. The other groups of sub-fractions were incubated with 0, 0.0625, 0.0125, 0.025, 0.05, 0.1 and 1.0 mmol/L of ALA. The incubation was done at 37°C for one hour. RESULTS: The effect of CoQ10 was mostly seen on the sperm progression at every minute of separation. The only exception was sample incubated in 3 μM of CoQ10 which shows significant increase on VCL over 0 μM and 6 μM of CoQ10 at 4 minutes of separation. It was also noted that 1 mM of ALA mostly improved sperm velocity when the separation was collected at two and four minutes. Sperm population incubated with 1 mM of ALA also showed significant changes on STR and LIN at four minutes of separation. CONCLUSION: CoQ10 and ALA influence sperm progression and velocity. This finding is important in the artificial reproductive techniques especially for in vitro fertilization (IVF). This will be further validated in future study.

Original languageEnglish
Pages (from-to)8-13
Number of pages6
JournalIIOAB Journal
Volume2
Issue number5
Publication statusPublished - Jul 2011

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coenzyme Q10
Thioctic Acid
Spermatozoa
Reproductive Techniques
Fertilization in Vitro
Free Radicals

Keywords

  • Antioxidant
  • Bovine
  • CASA
  • Kinematic
  • Sperm

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Bull spermatozoa motility : Optimization of coenzyme Q10 and alpha-lipoic acid concentration. / Ibrahim, Siti Fatimah; Farah Hanan Fathihah, Jaffar; Osman, Khairul; Mohamed, Syarifah Faezah Syed; Nang, Chew Fang; Ismail, Nur Hilwani; Ismail, Mohd Iswadi.

In: IIOAB Journal, Vol. 2, No. 5, 07.2011, p. 8-13.

Research output: Contribution to journalArticle

Ibrahim, Siti Fatimah ; Farah Hanan Fathihah, Jaffar ; Osman, Khairul ; Mohamed, Syarifah Faezah Syed ; Nang, Chew Fang ; Ismail, Nur Hilwani ; Ismail, Mohd Iswadi. / Bull spermatozoa motility : Optimization of coenzyme Q10 and alpha-lipoic acid concentration. In: IIOAB Journal. 2011 ; Vol. 2, No. 5. pp. 8-13.
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AU - Ibrahim, Siti Fatimah

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AU - Osman, Khairul

AU - Mohamed, Syarifah Faezah Syed

AU - Nang, Chew Fang

AU - Ismail, Nur Hilwani

AU - Ismail, Mohd Iswadi

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N2 - INTRODUCTION: Coenzyme Q10 (CoQ10) and alpha-lipoic acid (ALA) are antioxidants that play a role in ATP production and breakdown of free radicals. OBJECTIVE: The aim of this study was to optimize the concentration of CoQ10 and ALA based on motility pattern of spermatozoa. METHODS: The pooled bull spermatozoa were initially sub-fracted via electrophoretic separation which was performed at 20V for every two, four and six minutes. The sub-fractions were then further incubated with 0, 0.375, 0.75, 1.5, 3.0 and 6.0 μM of CoQ10. The other groups of sub-fractions were incubated with 0, 0.0625, 0.0125, 0.025, 0.05, 0.1 and 1.0 mmol/L of ALA. The incubation was done at 37°C for one hour. RESULTS: The effect of CoQ10 was mostly seen on the sperm progression at every minute of separation. The only exception was sample incubated in 3 μM of CoQ10 which shows significant increase on VCL over 0 μM and 6 μM of CoQ10 at 4 minutes of separation. It was also noted that 1 mM of ALA mostly improved sperm velocity when the separation was collected at two and four minutes. Sperm population incubated with 1 mM of ALA also showed significant changes on STR and LIN at four minutes of separation. CONCLUSION: CoQ10 and ALA influence sperm progression and velocity. This finding is important in the artificial reproductive techniques especially for in vitro fertilization (IVF). This will be further validated in future study.

AB - INTRODUCTION: Coenzyme Q10 (CoQ10) and alpha-lipoic acid (ALA) are antioxidants that play a role in ATP production and breakdown of free radicals. OBJECTIVE: The aim of this study was to optimize the concentration of CoQ10 and ALA based on motility pattern of spermatozoa. METHODS: The pooled bull spermatozoa were initially sub-fracted via electrophoretic separation which was performed at 20V for every two, four and six minutes. The sub-fractions were then further incubated with 0, 0.375, 0.75, 1.5, 3.0 and 6.0 μM of CoQ10. The other groups of sub-fractions were incubated with 0, 0.0625, 0.0125, 0.025, 0.05, 0.1 and 1.0 mmol/L of ALA. The incubation was done at 37°C for one hour. RESULTS: The effect of CoQ10 was mostly seen on the sperm progression at every minute of separation. The only exception was sample incubated in 3 μM of CoQ10 which shows significant increase on VCL over 0 μM and 6 μM of CoQ10 at 4 minutes of separation. It was also noted that 1 mM of ALA mostly improved sperm velocity when the separation was collected at two and four minutes. Sperm population incubated with 1 mM of ALA also showed significant changes on STR and LIN at four minutes of separation. CONCLUSION: CoQ10 and ALA influence sperm progression and velocity. This finding is important in the artificial reproductive techniques especially for in vitro fertilization (IVF). This will be further validated in future study.

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