Breaking-off tissue specific activity of the oil palm metallothionein-like gene promoter in T1 seedlings of tomato exposed to metal ions

Hossein Kamaladini, Siti Nor Akmar Abdullah, Maheran Abdul Aziz, Ismanizan Ismail, Fatemeh Haddadi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Metallothioneins (MTs) are cysteine-rich metal-binding proteins that are involved in cell growth regulation, transportation of metal ions and detoxification of heavy metals. A mesocarp-specific metallothionein-like gene (MT3-A) promoter was isolated from the oil palm (Elaeis guineensis Jacq). A vector construct containing the MT3-A promoter fused to the β-glucuronidase (GUS) gene in the pCAMBIA 1304 vector was produced and used in Agrobacterium-mediated transformation of tomato. Histochemical GUS assay of different tissues of transgenic tomato showed that the MT3-A promoter only drove GUS expression in the reproductive tissues and organs, including the anther, fruit and seed coat. Competitive RT-PCR and GUS fluorometric assay showed changes in the level of GUS mRNA and enzyme activity in the transgenic tomato (T0). No GUS mRNA was found in roots and leaves of transgenic tomato. In contrast, the leaves of transgenic tomato seedlings (T1) produced the highest GUS activity when treated with 150μM Cu2+ compared to the control (without Cu2+). However, Zn2+ and Fe2+ treatments did not show GUS expression in the leaves of the transgenic tomato seedlings. Interestingly, the results showed a breaking-off tissue-specific activity of the oil palm MT3-A promoter in T1 seedlings of tomato when subjected to Cu2+ ions.

Original languageEnglish
Pages (from-to)346-354
Number of pages9
JournalJournal of Plant Physiology
Volume170
Issue number3
DOIs
Publication statusPublished - 15 Feb 2013

Fingerprint

Metallothionein
metallothionein
Glucuronidase
Elaeis guineensis
Lycopersicon esculentum
metal ions
Seedlings
Oils
Metals
promoter regions
tomatoes
Ions
seedlings
genetically modified organisms
Genes
genes
leaves
mesocarp
Messenger RNA
Agrobacterium

Keywords

  • Competitive RT-PCR
  • GUS fluorometric assay
  • Metallothionein gene promoter
  • Oil palm
  • Tomato

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science

Cite this

Breaking-off tissue specific activity of the oil palm metallothionein-like gene promoter in T1 seedlings of tomato exposed to metal ions. / Kamaladini, Hossein; Nor Akmar Abdullah, Siti; Aziz, Maheran Abdul; Ismail, Ismanizan; Haddadi, Fatemeh.

In: Journal of Plant Physiology, Vol. 170, No. 3, 15.02.2013, p. 346-354.

Research output: Contribution to journalArticle

Kamaladini, Hossein ; Nor Akmar Abdullah, Siti ; Aziz, Maheran Abdul ; Ismail, Ismanizan ; Haddadi, Fatemeh. / Breaking-off tissue specific activity of the oil palm metallothionein-like gene promoter in T1 seedlings of tomato exposed to metal ions. In: Journal of Plant Physiology. 2013 ; Vol. 170, No. 3. pp. 346-354.
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AB - Metallothioneins (MTs) are cysteine-rich metal-binding proteins that are involved in cell growth regulation, transportation of metal ions and detoxification of heavy metals. A mesocarp-specific metallothionein-like gene (MT3-A) promoter was isolated from the oil palm (Elaeis guineensis Jacq). A vector construct containing the MT3-A promoter fused to the β-glucuronidase (GUS) gene in the pCAMBIA 1304 vector was produced and used in Agrobacterium-mediated transformation of tomato. Histochemical GUS assay of different tissues of transgenic tomato showed that the MT3-A promoter only drove GUS expression in the reproductive tissues and organs, including the anther, fruit and seed coat. Competitive RT-PCR and GUS fluorometric assay showed changes in the level of GUS mRNA and enzyme activity in the transgenic tomato (T0). No GUS mRNA was found in roots and leaves of transgenic tomato. In contrast, the leaves of transgenic tomato seedlings (T1) produced the highest GUS activity when treated with 150μM Cu2+ compared to the control (without Cu2+). However, Zn2+ and Fe2+ treatments did not show GUS expression in the leaves of the transgenic tomato seedlings. Interestingly, the results showed a breaking-off tissue-specific activity of the oil palm MT3-A promoter in T1 seedlings of tomato when subjected to Cu2+ ions.

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