Bovine serum albumin

Survival and osmolarity effect in bovine spermatozoa stored above freezing point

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Liquid nitrogen preservation in remote farms is a limitation. The goal of this study was to determine optimum temperature above freezing point for bovine spermatozoa preservation using bovine serum albumin (BSA) as a supplementation. Pooled semen sample from three ejaculates was subjected to various BSA concentration (1, 4, 8 and 12mgml -1), before incubation in different above freezing point temperatures (4, 25 and 37°C). Viability assessment was carried out against time from day 0 (fresh sample) until all spermatozoa become nonviable. Optimal condition for bovine spermatozoa storage was at 4°C with 1mgml -1 BSA for almost 7days. BSA improved bovine spermatozoa viability declining rate to 44.28% at day 4 and 57.59% at day 7 compared to control, with 80.54% and 98.57% at day 4 and 7 respectively. Increase in BSA concentration did not improve sperm viability. Our results also confirmed that there was a strong negative correlation between media osmolarity and bovine spermatozoa survival rate with r=0.885, P<0.0001. Bovine serum albumin helps to improve survival rate of bovine spermatozoa stored above freezing point.

Original languageEnglish
Pages (from-to)447-453
Number of pages7
JournalAndrologia
Volume44
Issue numberSUPPL.1
DOIs
Publication statusPublished - May 2012

Fingerprint

Bovine Serum Albumin
Osmolar Concentration
Freezing
Spermatozoa
Transition Temperature
Semen
Nitrogen

Keywords

  • Liquid nitrogen
  • Osmosis
  • Preservation
  • Sperm viability

ASJC Scopus subject areas

  • Urology
  • Endocrinology

Cite this

Bovine serum albumin : Survival and osmolarity effect in bovine spermatozoa stored above freezing point. / Nang, C. F.; Osman, Khairul; Budin, Siti Balkis; Ismail, M. I.; Farah Hanan Fathihah, Jaffar; Mohamad, S. F S; Ibrahim, Siti Fatimah.

In: Andrologia, Vol. 44, No. SUPPL.1, 05.2012, p. 447-453.

Research output: Contribution to journalArticle

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