Bovine serum albumin-loaded chitosan/dextran nanoparticles: Preparation and evaluation of ex vivo colloidal stability in serum

Haliza Katas, Zahid Hussain, Siti Asarida Awang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Chitosan (CS) nanoparticles have several distinct intrinsic advantages; however, their in vivo colloidal stability in biological fluids was not fully explored especially when carrying proteins. The present study aimed to investigate their colloidal stability using an ex vivo physiological model of fetal bovine serum (FBS) and human serum (HS). The stability of bovine-serum-albumin (BSA-) loaded nanoparticles was relatively higher in FBS than that in HS. Particle size of unloaded and BSA-loaded nanoparticles was statistically unchanged up to 24 h after incubation in FBS. However in HS, a significant increase in particle size from 144 ± 17 to 711 ± 22 nm was observed for unloaded nanoparticles and by 2.5-fold for BSA-loaded nanoparticle, at 24 h after incubation in HS. Zeta potential of both nanoparticles was less affected by the components in FBS compared to those in HS. A remarkable swelling extent was experienced for unloaded and BSA-loaded nanoparticles in HS, up to 54 ± 4% and 44 ± 5%, respectively. Morphology of unloaded and BSA-loaded nanoparticles was varied from smooth spherical and rod shape to irregular shape when incubated in FBS; however, form agglomerates when incubated in HS. These findings therefore suggest that HS is more reactive to cause colloidal instability to the chitosan nanoparticles compared to FBS.

Original languageEnglish
Article number536291
JournalJournal of Nanomaterials
Volume2013
DOIs
Publication statusPublished - 2013

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Dextran
Chitosan
Bovine Serum Albumin
Dextrans
Nanoparticles
Particle size
Physiological models
Zeta potential
Swelling
Proteins
Fluids

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Bovine serum albumin-loaded chitosan/dextran nanoparticles : Preparation and evaluation of ex vivo colloidal stability in serum. / Katas, Haliza; Hussain, Zahid; Awang, Siti Asarida.

In: Journal of Nanomaterials, Vol. 2013, 536291, 2013.

Research output: Contribution to journalArticle

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