Boosting enzymatic hydrolysis of pressurized ammonium hydroxide pretreated empty fruit bunch using response surface methodology

N. U.R. Farahin Abdul Rahman, Shuhaida Harun, Sajab Mohd Shaiful, Saiful Irwan Zubairi, Masturah Markom, Jamaliah Md Jahim, Mohd Tusirin Mohd Nor, Mohd Amrin Abdullah, Norhafizi Hashim

Research output: Contribution to journalArticle

Abstract

Oil palm Empty Fruit Bunch (EFB) was pretreated using Pressurized Ammonium Hydroxide (PAH) and was employed as Lignocellulosic Biomass (LCB) substrate for the investigation on the monomeric fermentable sugar production using the enzymatic hydrolysis process. Cellulose saccharification in enzymatic hydrolysis into a high yield fermentable sugar is an important step in Biochemical Conversion Technology (BCT). In order to determine the optimum variable conditions that can produce a high yield of fermentable sugar, a statistical approach using Response Surface Methodology (RSM) was performed in this study. Three independent variables, enzyme loading (15-50 FPU/g glucan), hydrolysis temperature (45-60°C), and agitation of the hydrolysis process (100-180 rpm) were investigated at five different levels (-α,-1, 0, +1, +α) of operating conditions and the experimental conditions were randomly setup using the Design of Experiment software. The regression models indicated that R2 for glucose and xylose concentration was 95 and 88% showing the experimental variations were well defined by the models. For the lack of fit test, with p-values > 0.05 for both concentration sugars, 0.218 for glucose and 0.055 for xylose, it proves that the model was significant to the prediction models. The optimal conditions for the enzymatic hydrolysis of the EFB were determined at 32.5 FPU/g of glucan of enzyme loading, 50°C of hydrolysis temperature, and 140 rpm of agitation speed. The validation of the model at the optimum conditions produced a maximum glucose concentration of 8.78 ± 0.01 g/L (conversion of 81.7 ± 0.02 %, and yield of 332.95 ± 0.98 g/kg dry EFB), with a corresponding xylose concentration of 4.40 ± 0.01 g/L (conversion of 57 ± 0.35% and yield of 173.72 g/kg dry EFB).

Original languageEnglish
Pages (from-to)2421-2445
Number of pages25
JournalJournal of Engineering Science and Technology
Volume13
Issue number8
Publication statusPublished - 1 Aug 2018

Fingerprint

Ammonium hydroxide
Enzymatic hydrolysis
Fruits
Sugars
Xylose
Glucose
Hydrolysis
Enzymes
Saccharification
Palm oil
Design of experiments
Cellulose
Biomass
Temperature
Substrates

Keywords

  • Ammonia
  • Empty fruit bunch
  • Lignocellulosic
  • Pressurized
  • Pretreatment
  • RSM
  • Sugar concentration

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Boosting enzymatic hydrolysis of pressurized ammonium hydroxide pretreated empty fruit bunch using response surface methodology. / Farahin Abdul Rahman, N. U.R.; Harun, Shuhaida; Mohd Shaiful, Sajab; Zubairi, Saiful Irwan; Markom, Masturah; Md Jahim, Jamaliah; Tusirin Mohd Nor, Mohd; Amrin Abdullah, Mohd; Hashim, Norhafizi.

In: Journal of Engineering Science and Technology, Vol. 13, No. 8, 01.08.2018, p. 2421-2445.

Research output: Contribution to journalArticle

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abstract = "Oil palm Empty Fruit Bunch (EFB) was pretreated using Pressurized Ammonium Hydroxide (PAH) and was employed as Lignocellulosic Biomass (LCB) substrate for the investigation on the monomeric fermentable sugar production using the enzymatic hydrolysis process. Cellulose saccharification in enzymatic hydrolysis into a high yield fermentable sugar is an important step in Biochemical Conversion Technology (BCT). In order to determine the optimum variable conditions that can produce a high yield of fermentable sugar, a statistical approach using Response Surface Methodology (RSM) was performed in this study. Three independent variables, enzyme loading (15-50 FPU/g glucan), hydrolysis temperature (45-60°C), and agitation of the hydrolysis process (100-180 rpm) were investigated at five different levels (-α,-1, 0, +1, +α) of operating conditions and the experimental conditions were randomly setup using the Design of Experiment software. The regression models indicated that R2 for glucose and xylose concentration was 95 and 88{\%} showing the experimental variations were well defined by the models. For the lack of fit test, with p-values > 0.05 for both concentration sugars, 0.218 for glucose and 0.055 for xylose, it proves that the model was significant to the prediction models. The optimal conditions for the enzymatic hydrolysis of the EFB were determined at 32.5 FPU/g of glucan of enzyme loading, 50°C of hydrolysis temperature, and 140 rpm of agitation speed. The validation of the model at the optimum conditions produced a maximum glucose concentration of 8.78 ± 0.01 g/L (conversion of 81.7 ± 0.02 {\%}, and yield of 332.95 ± 0.98 g/kg dry EFB), with a corresponding xylose concentration of 4.40 ± 0.01 g/L (conversion of 57 ± 0.35{\%} and yield of 173.72 g/kg dry EFB).",
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AU - Farahin Abdul Rahman, N. U.R.

AU - Harun, Shuhaida

AU - Mohd Shaiful, Sajab

AU - Zubairi, Saiful Irwan

AU - Markom, Masturah

AU - Md Jahim, Jamaliah

AU - Tusirin Mohd Nor, Mohd

AU - Amrin Abdullah, Mohd

AU - Hashim, Norhafizi

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AB - Oil palm Empty Fruit Bunch (EFB) was pretreated using Pressurized Ammonium Hydroxide (PAH) and was employed as Lignocellulosic Biomass (LCB) substrate for the investigation on the monomeric fermentable sugar production using the enzymatic hydrolysis process. Cellulose saccharification in enzymatic hydrolysis into a high yield fermentable sugar is an important step in Biochemical Conversion Technology (BCT). In order to determine the optimum variable conditions that can produce a high yield of fermentable sugar, a statistical approach using Response Surface Methodology (RSM) was performed in this study. Three independent variables, enzyme loading (15-50 FPU/g glucan), hydrolysis temperature (45-60°C), and agitation of the hydrolysis process (100-180 rpm) were investigated at five different levels (-α,-1, 0, +1, +α) of operating conditions and the experimental conditions were randomly setup using the Design of Experiment software. The regression models indicated that R2 for glucose and xylose concentration was 95 and 88% showing the experimental variations were well defined by the models. For the lack of fit test, with p-values > 0.05 for both concentration sugars, 0.218 for glucose and 0.055 for xylose, it proves that the model was significant to the prediction models. The optimal conditions for the enzymatic hydrolysis of the EFB were determined at 32.5 FPU/g of glucan of enzyme loading, 50°C of hydrolysis temperature, and 140 rpm of agitation speed. The validation of the model at the optimum conditions produced a maximum glucose concentration of 8.78 ± 0.01 g/L (conversion of 81.7 ± 0.02 %, and yield of 332.95 ± 0.98 g/kg dry EFB), with a corresponding xylose concentration of 4.40 ± 0.01 g/L (conversion of 57 ± 0.35% and yield of 173.72 g/kg dry EFB).

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KW - Pressurized

KW - Pretreatment

KW - RSM

KW - Sugar concentration

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