Bone turnover and oxidative stress markers in estrogen-deficient rats treated with Marantodes pumilum leaves and roots

A comparative study

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Abstract

Purpose: To investigate the relative changes in bone turnover and oxidative status markers of estrogen-deficient rats treated with Marantodes pumilum var. alata (MPva) leaf and root extracts. Methods: Thirty-six female Sprague-Dawley rats were divided into 6 groups (n = 6), namely: baseline (BL); placebo surgery (PS); ovariectomized control (OVXC); estrogen treatment (ERT); leaf extract treatment (MPv); and root extract treatment (MPr). Rats in all groups, except BL and PS, were ovariectomized to induce menopause. Through the oral route, ERT received 64.5 µg/kg/day estrogen (Premarin®); MPv received 20 mg/kg/day leaf extracts of MPva; MPr received 20 mg/kg/day root extract of MPva; while BL, OVXC, and PS served as untreated controls. At the end of 8 weeks treatment, blood and bone samples were collected for assay of bone turnover markers (osteocalcin and pyridinoline) and oxidative status markers (4-hydroxynonenal, superoxide dismutase, and glutathione peroxidase) using enzyme-linked immunosorbent assay (ELISA). Results: Significantly higher (p < 0.05) bone level of glutathione peroxidase was seen in MPv when compared with BL and OVXC. MPv also showed lower serum level of pyridinoline (p<0.05) compared to OVXC. Bone level of 4-hydroxynonenal in both MPv and MPr groups was significantly reduced (p < 0.05) when compared with OVXC. Conclusion: MPva leaf showed more remarkable effects on bone turnover and oxidative stress markers of ovariectomized rats than its roots and estrogen treatment. Thus, the use of MPva leaf, as an alternative to estrogen for treating postmenopausal osteoporosis, would be preferred to its roots.

Original languageEnglish
Pages (from-to)611-617
Number of pages7
JournalTropical Journal of Pharmaceutical Research
Volume17
Issue number4
DOIs
Publication statusPublished - 1 Apr 2018

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Bone Remodeling
Estrogens
Oxidative Stress
Placebos
Glutathione Peroxidase
Bone and Bones
Therapeutics
Conjugated (USP) Estrogens
Postmenopausal Osteoporosis
Osteocalcin
Menopause
Superoxide Dismutase
Sprague Dawley Rats
Enzyme-Linked Immunosorbent Assay
Serum

Keywords

  • Marantodes pumilum
  • Osteoporosis
  • Phytoestrogens

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology (medical)

Cite this

@article{cfc900b99bf24c4bae58e15c65522065,
title = "Bone turnover and oxidative stress markers in estrogen-deficient rats treated with Marantodes pumilum leaves and roots: A comparative study",
abstract = "Purpose: To investigate the relative changes in bone turnover and oxidative status markers of estrogen-deficient rats treated with Marantodes pumilum var. alata (MPva) leaf and root extracts. Methods: Thirty-six female Sprague-Dawley rats were divided into 6 groups (n = 6), namely: baseline (BL); placebo surgery (PS); ovariectomized control (OVXC); estrogen treatment (ERT); leaf extract treatment (MPv); and root extract treatment (MPr). Rats in all groups, except BL and PS, were ovariectomized to induce menopause. Through the oral route, ERT received 64.5 µg/kg/day estrogen (Premarin{\circledR}); MPv received 20 mg/kg/day leaf extracts of MPva; MPr received 20 mg/kg/day root extract of MPva; while BL, OVXC, and PS served as untreated controls. At the end of 8 weeks treatment, blood and bone samples were collected for assay of bone turnover markers (osteocalcin and pyridinoline) and oxidative status markers (4-hydroxynonenal, superoxide dismutase, and glutathione peroxidase) using enzyme-linked immunosorbent assay (ELISA). Results: Significantly higher (p < 0.05) bone level of glutathione peroxidase was seen in MPv when compared with BL and OVXC. MPv also showed lower serum level of pyridinoline (p<0.05) compared to OVXC. Bone level of 4-hydroxynonenal in both MPv and MPr groups was significantly reduced (p < 0.05) when compared with OVXC. Conclusion: MPva leaf showed more remarkable effects on bone turnover and oxidative stress markers of ovariectomized rats than its roots and estrogen treatment. Thus, the use of MPva leaf, as an alternative to estrogen for treating postmenopausal osteoporosis, would be preferred to its roots.",
keywords = "Marantodes pumilum, Osteoporosis, Phytoestrogens",
author = "Giaze, {Tijjani Rabiu} and Shuid, {Ahmad Nazrun} and Soelaiman, {Ima Nirwana} and Norliza Muhammad and Aladin, {Nor Ashila} and Norazlina Mohamed",
year = "2018",
month = "4",
day = "1",
doi = "10.4314/tjpr.v17i4.7",
language = "English",
volume = "17",
pages = "611--617",
journal = "Tropical Journal of Pharmaceutical Research",
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TY - JOUR

T1 - Bone turnover and oxidative stress markers in estrogen-deficient rats treated with Marantodes pumilum leaves and roots

T2 - A comparative study

AU - Giaze, Tijjani Rabiu

AU - Shuid, Ahmad Nazrun

AU - Soelaiman, Ima Nirwana

AU - Muhammad, Norliza

AU - Aladin, Nor Ashila

AU - Mohamed, Norazlina

PY - 2018/4/1

Y1 - 2018/4/1

N2 - Purpose: To investigate the relative changes in bone turnover and oxidative status markers of estrogen-deficient rats treated with Marantodes pumilum var. alata (MPva) leaf and root extracts. Methods: Thirty-six female Sprague-Dawley rats were divided into 6 groups (n = 6), namely: baseline (BL); placebo surgery (PS); ovariectomized control (OVXC); estrogen treatment (ERT); leaf extract treatment (MPv); and root extract treatment (MPr). Rats in all groups, except BL and PS, were ovariectomized to induce menopause. Through the oral route, ERT received 64.5 µg/kg/day estrogen (Premarin®); MPv received 20 mg/kg/day leaf extracts of MPva; MPr received 20 mg/kg/day root extract of MPva; while BL, OVXC, and PS served as untreated controls. At the end of 8 weeks treatment, blood and bone samples were collected for assay of bone turnover markers (osteocalcin and pyridinoline) and oxidative status markers (4-hydroxynonenal, superoxide dismutase, and glutathione peroxidase) using enzyme-linked immunosorbent assay (ELISA). Results: Significantly higher (p < 0.05) bone level of glutathione peroxidase was seen in MPv when compared with BL and OVXC. MPv also showed lower serum level of pyridinoline (p<0.05) compared to OVXC. Bone level of 4-hydroxynonenal in both MPv and MPr groups was significantly reduced (p < 0.05) when compared with OVXC. Conclusion: MPva leaf showed more remarkable effects on bone turnover and oxidative stress markers of ovariectomized rats than its roots and estrogen treatment. Thus, the use of MPva leaf, as an alternative to estrogen for treating postmenopausal osteoporosis, would be preferred to its roots.

AB - Purpose: To investigate the relative changes in bone turnover and oxidative status markers of estrogen-deficient rats treated with Marantodes pumilum var. alata (MPva) leaf and root extracts. Methods: Thirty-six female Sprague-Dawley rats were divided into 6 groups (n = 6), namely: baseline (BL); placebo surgery (PS); ovariectomized control (OVXC); estrogen treatment (ERT); leaf extract treatment (MPv); and root extract treatment (MPr). Rats in all groups, except BL and PS, were ovariectomized to induce menopause. Through the oral route, ERT received 64.5 µg/kg/day estrogen (Premarin®); MPv received 20 mg/kg/day leaf extracts of MPva; MPr received 20 mg/kg/day root extract of MPva; while BL, OVXC, and PS served as untreated controls. At the end of 8 weeks treatment, blood and bone samples were collected for assay of bone turnover markers (osteocalcin and pyridinoline) and oxidative status markers (4-hydroxynonenal, superoxide dismutase, and glutathione peroxidase) using enzyme-linked immunosorbent assay (ELISA). Results: Significantly higher (p < 0.05) bone level of glutathione peroxidase was seen in MPv when compared with BL and OVXC. MPv also showed lower serum level of pyridinoline (p<0.05) compared to OVXC. Bone level of 4-hydroxynonenal in both MPv and MPr groups was significantly reduced (p < 0.05) when compared with OVXC. Conclusion: MPva leaf showed more remarkable effects on bone turnover and oxidative stress markers of ovariectomized rats than its roots and estrogen treatment. Thus, the use of MPva leaf, as an alternative to estrogen for treating postmenopausal osteoporosis, would be preferred to its roots.

KW - Marantodes pumilum

KW - Osteoporosis

KW - Phytoestrogens

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