Bone oxidative changes during early fracture healing of postmenopausal osteoporosis rat model

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Abstract

Ovariectomised rat is an accepted model of postmenopausal osteoporosis. Fracture can be induced in this model to simulate osteoporotic fracture. The aim of the study was to determine the anti-oxidative changes during early fracture healing in postmenopausal osteoporosis rat model. The lipid per oxidation levels and anti-oxidant enzyme activities during the early phase of osteoporotic fracture healing were measured directly in the fractured bone of ovariectomised rats. The effects of antioxidant supplementation i.e., vitamin E, on the oxidative parameters were also measured. Thirty two female Sprague-Dawley rats were divided into groups of Sham-Operated (SO), Ovariectomised-Control (OVC), ovariectomised +60 mg kg -1 of a-tocopherol (ATF) and ovariectomised +60 mg kg -1 of Tocotrienol-rich fraction (TT). The rats were left untreated for 2 months to allow ovariectomy-induced osteoporosis before their right femora were fractured at the mid-diaphysis. After 2 weeks of treatment, the Thiob arbituric Acid Reactive Substances (TBARS) level and Super oxide Dismutase (SOD) and Glutathione Peroxides (GPX) enzyme activities of the fractured femora were measured. There was a significant drop in the SOD activity of OVC group compared to SO group. Tocotrienol was able to significantly lower the TBARS level and raise the GPX activity compared to OVC group. While, a-tocopherol was able to significantly raise the SOD activity compared to OVC group. Therefore, only SOD activity was affected during early fracture healing in this rat model. Tocotrienol was able to reduce lipid per oxidation during early fracture healing of osteoporotic bone probably by enhancing its GPX activity. Anti-oxidants were beneficial in improving the bone oxidative status during early fracture healing.

Original languageEnglish
Pages (from-to)1193-1203
Number of pages11
JournalAsian Journal of Animal and Veterinary Advances
Volume6
Issue number12
DOIs
Publication statusPublished - 2011

Fingerprint

Postmenopausal Osteoporosis
Fracture Healing
osteoporosis
tocotrienols
Tocotrienols
superoxide dismutase
animal models
peroxides
Oxides
bones
Peroxides
Bone and Bones
glutathione
rats
Glutathione
femur
tocopherols
Osteoporotic Fractures
oxidants
Tocopherols

Keywords

  • Fracture
  • Osteoporosis
  • Ovariectomy
  • Oxidative stress
  • Tocotrienol
  • Vitamin E

ASJC Scopus subject areas

  • veterinary(all)
  • Animal Science and Zoology

Cite this

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title = "Bone oxidative changes during early fracture healing of postmenopausal osteoporosis rat model",
abstract = "Ovariectomised rat is an accepted model of postmenopausal osteoporosis. Fracture can be induced in this model to simulate osteoporotic fracture. The aim of the study was to determine the anti-oxidative changes during early fracture healing in postmenopausal osteoporosis rat model. The lipid per oxidation levels and anti-oxidant enzyme activities during the early phase of osteoporotic fracture healing were measured directly in the fractured bone of ovariectomised rats. The effects of antioxidant supplementation i.e., vitamin E, on the oxidative parameters were also measured. Thirty two female Sprague-Dawley rats were divided into groups of Sham-Operated (SO), Ovariectomised-Control (OVC), ovariectomised +60 mg kg -1 of a-tocopherol (ATF) and ovariectomised +60 mg kg -1 of Tocotrienol-rich fraction (TT). The rats were left untreated for 2 months to allow ovariectomy-induced osteoporosis before their right femora were fractured at the mid-diaphysis. After 2 weeks of treatment, the Thiob arbituric Acid Reactive Substances (TBARS) level and Super oxide Dismutase (SOD) and Glutathione Peroxides (GPX) enzyme activities of the fractured femora were measured. There was a significant drop in the SOD activity of OVC group compared to SO group. Tocotrienol was able to significantly lower the TBARS level and raise the GPX activity compared to OVC group. While, a-tocopherol was able to significantly raise the SOD activity compared to OVC group. Therefore, only SOD activity was affected during early fracture healing in this rat model. Tocotrienol was able to reduce lipid per oxidation during early fracture healing of osteoporotic bone probably by enhancing its GPX activity. Anti-oxidants were beneficial in improving the bone oxidative status during early fracture healing.",
keywords = "Fracture, Osteoporosis, Ovariectomy, Oxidative stress, Tocotrienol, Vitamin E",
author = "Shuid, {Ahmad Nazrun} and Sharlina Mohamad and Norazlina Mohamed and Mokhtar, {Sabarul Afian} and Norliza Muhammad and Soelaiman, {Ima Nirwana}",
year = "2011",
doi = "10.3923/ajava.2011.1193.1203",
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volume = "6",
pages = "1193--1203",
journal = "Asian Journal of Animal and Veterinary Advances",
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T1 - Bone oxidative changes during early fracture healing of postmenopausal osteoporosis rat model

AU - Shuid, Ahmad Nazrun

AU - Mohamad, Sharlina

AU - Mohamed, Norazlina

AU - Mokhtar, Sabarul Afian

AU - Muhammad, Norliza

AU - Soelaiman, Ima Nirwana

PY - 2011

Y1 - 2011

N2 - Ovariectomised rat is an accepted model of postmenopausal osteoporosis. Fracture can be induced in this model to simulate osteoporotic fracture. The aim of the study was to determine the anti-oxidative changes during early fracture healing in postmenopausal osteoporosis rat model. The lipid per oxidation levels and anti-oxidant enzyme activities during the early phase of osteoporotic fracture healing were measured directly in the fractured bone of ovariectomised rats. The effects of antioxidant supplementation i.e., vitamin E, on the oxidative parameters were also measured. Thirty two female Sprague-Dawley rats were divided into groups of Sham-Operated (SO), Ovariectomised-Control (OVC), ovariectomised +60 mg kg -1 of a-tocopherol (ATF) and ovariectomised +60 mg kg -1 of Tocotrienol-rich fraction (TT). The rats were left untreated for 2 months to allow ovariectomy-induced osteoporosis before their right femora were fractured at the mid-diaphysis. After 2 weeks of treatment, the Thiob arbituric Acid Reactive Substances (TBARS) level and Super oxide Dismutase (SOD) and Glutathione Peroxides (GPX) enzyme activities of the fractured femora were measured. There was a significant drop in the SOD activity of OVC group compared to SO group. Tocotrienol was able to significantly lower the TBARS level and raise the GPX activity compared to OVC group. While, a-tocopherol was able to significantly raise the SOD activity compared to OVC group. Therefore, only SOD activity was affected during early fracture healing in this rat model. Tocotrienol was able to reduce lipid per oxidation during early fracture healing of osteoporotic bone probably by enhancing its GPX activity. Anti-oxidants were beneficial in improving the bone oxidative status during early fracture healing.

AB - Ovariectomised rat is an accepted model of postmenopausal osteoporosis. Fracture can be induced in this model to simulate osteoporotic fracture. The aim of the study was to determine the anti-oxidative changes during early fracture healing in postmenopausal osteoporosis rat model. The lipid per oxidation levels and anti-oxidant enzyme activities during the early phase of osteoporotic fracture healing were measured directly in the fractured bone of ovariectomised rats. The effects of antioxidant supplementation i.e., vitamin E, on the oxidative parameters were also measured. Thirty two female Sprague-Dawley rats were divided into groups of Sham-Operated (SO), Ovariectomised-Control (OVC), ovariectomised +60 mg kg -1 of a-tocopherol (ATF) and ovariectomised +60 mg kg -1 of Tocotrienol-rich fraction (TT). The rats were left untreated for 2 months to allow ovariectomy-induced osteoporosis before their right femora were fractured at the mid-diaphysis. After 2 weeks of treatment, the Thiob arbituric Acid Reactive Substances (TBARS) level and Super oxide Dismutase (SOD) and Glutathione Peroxides (GPX) enzyme activities of the fractured femora were measured. There was a significant drop in the SOD activity of OVC group compared to SO group. Tocotrienol was able to significantly lower the TBARS level and raise the GPX activity compared to OVC group. While, a-tocopherol was able to significantly raise the SOD activity compared to OVC group. Therefore, only SOD activity was affected during early fracture healing in this rat model. Tocotrienol was able to reduce lipid per oxidation during early fracture healing of osteoporotic bone probably by enhancing its GPX activity. Anti-oxidants were beneficial in improving the bone oxidative status during early fracture healing.

KW - Fracture

KW - Osteoporosis

KW - Ovariectomy

KW - Oxidative stress

KW - Tocotrienol

KW - Vitamin E

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