Bone micro-CT assessments in an orchidectomised rat model supplemented with Eurycoma longifolia

Rosmaliza Ramli, Mohd Fadhli Khamis, Ahmad Nazrun Shuid

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Recent studies suggested that Eurycoma longifolia, a herbal plant, may have the potential to treat osteoporosis in elderly male. This study aimed to determine the effects of Eurycoma longifolia supplementation on the trabecular bone microarchitecture of orchidectomised rats (androgen-deficient osteoporosis model). Forty-eight-aged (1012 months old) Sprague Dawley rats were divided into six groups of sham-operated (SHAM), orchidectomised control (ORX), orchidectomised + 7 mg/rat testosterone enanthate (TEN) and orchidectomised + Eurycoma longifolia 30 mg/kg (EL30), orchidectomised + Eurycoma longifolia 60 mg/kg (EL60), orchidectomised + Eurycoma longifolia 90 mg/kg (EL90). Rats were euthanized following six weeks of treatment. The left femora were used to measure the trabecular bone microarchitecture using micro-CT. Orchidectomy significantly decreased connectivity density, trabecular bone volume, and trabecular number compared to the SHAM group. Testosterone replacement reversed all the orchidectomy-induced changes in the micro-CT parameters. EL at 30 and 60 mg/kg rat worsened the trabecular bone connectivity density and trabecular separation parameters of orchidectomised rats. EL at 90 mg/kg rat preserved the bone volume. High dose of EL (90 mg/kg) may have potential in preserving the bone microarchitecture of orchidectomised rats, but lower doses may further worsen the osteoporotic changes.

Original languageEnglish
Article number501858
JournalEvidence-based Complementary and Alternative Medicine
Volume2012
DOIs
Publication statusPublished - 2012

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Eurycoma
Bone and Bones
Orchiectomy
Osteoporosis
Bone Density
Femur
Androgens
Sprague Dawley Rats
Testosterone

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Bone micro-CT assessments in an orchidectomised rat model supplemented with Eurycoma longifolia. / Ramli, Rosmaliza; Khamis, Mohd Fadhli; Shuid, Ahmad Nazrun.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2012, 501858, 2012.

Research output: Contribution to journalArticle

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