Bone marrow and adipose stem cells can be tracked with PKH26 until post staining passage 6 in in vitro and in vivo

C. C. Ude, B. S. Shamsul, Min Hwei Ng, H. C. Chen, Nor Hamdan Mohamad Yahaya, B. S. Aminuddin, Ruszymah Idrus

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Tracking of transplanted cells has become an important procedure in cell therapy. We studied the in vitro dye retention, survival and in vivo tracking of stem cells with PKH26 dye. Sheep BMSCs and ADSCs were labeled with 2, 4 and 8 μmol of PKH26 and monitored for six passages. Labeled BMSCs and ADSCs acquired mean cumulative population doubling of 12.7 ± 0.4 and 14.6 ± 0.5; unlabeled samples had 13.8 ± 0.5 and 15.4 ± 0.6 respectively. Upon staining with 2, 4 and 8 μmol PKH26, BMSCs had retentions of 40.0 ± 5.8, 60.0 ± 2.9 and 95.0 ± 2.9%, while ADSCs had 92.0 ± 1.2, 95.0 ± 1.2 and 98.0 ± 1.2%. ADSCs retentions were significantly higher at 2 and 4 μmol. On dye retention comparison at 8 μmol and 4 μmol for BMSCs and ADSCs; ADSCs were significantly higher at passages 2 and 3. The viability of BMSCs reduced from 94.0 ± 1.2% to 90.0 ± 0.6% and ADSCs from 94.0 ± 1.2% to 52.0 ± 1.2% (p< 0.05) after 24. h. BMSCs had significant up regulation of the cartilage genes for both the labeled and the unlabeled samples compared to ADSCs (p< 0.05). PKH26 fluorescence was detected on the resected portions of the regenerated neo-cartilage. The recommended concentration of PKH26 for ADSCs is 2 μmol and BMSCs is 8 μmol, and they can be tracked up to 49 days.

Original languageEnglish
Pages (from-to)156-163
Number of pages8
JournalTissue and Cell
Volume44
Issue number3
DOIs
Publication statusPublished - Jun 2012

Fingerprint

Stem Cells
Bone Marrow
Staining and Labeling
Coloring Agents
Cartilage
Cell Tracking
Cell- and Tissue-Based Therapy
Sheep
Up-Regulation
Fluorescence
In Vitro Techniques
PKH 26
Population
Genes

Keywords

  • Adipose stem cells
  • Bone marrow stem cells
  • Cell tracking
  • PKH26 retention
  • Tissue engineering
  • Viability

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology

Cite this

Bone marrow and adipose stem cells can be tracked with PKH26 until post staining passage 6 in in vitro and in vivo. / Ude, C. C.; Shamsul, B. S.; Ng, Min Hwei; Chen, H. C.; Mohamad Yahaya, Nor Hamdan; Aminuddin, B. S.; Idrus, Ruszymah.

In: Tissue and Cell, Vol. 44, No. 3, 06.2012, p. 156-163.

Research output: Contribution to journalArticle

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abstract = "Tracking of transplanted cells has become an important procedure in cell therapy. We studied the in vitro dye retention, survival and in vivo tracking of stem cells with PKH26 dye. Sheep BMSCs and ADSCs were labeled with 2, 4 and 8 μmol of PKH26 and monitored for six passages. Labeled BMSCs and ADSCs acquired mean cumulative population doubling of 12.7 ± 0.4 and 14.6 ± 0.5; unlabeled samples had 13.8 ± 0.5 and 15.4 ± 0.6 respectively. Upon staining with 2, 4 and 8 μmol PKH26, BMSCs had retentions of 40.0 ± 5.8, 60.0 ± 2.9 and 95.0 ± 2.9{\%}, while ADSCs had 92.0 ± 1.2, 95.0 ± 1.2 and 98.0 ± 1.2{\%}. ADSCs retentions were significantly higher at 2 and 4 μmol. On dye retention comparison at 8 μmol and 4 μmol for BMSCs and ADSCs; ADSCs were significantly higher at passages 2 and 3. The viability of BMSCs reduced from 94.0 ± 1.2{\%} to 90.0 ± 0.6{\%} and ADSCs from 94.0 ± 1.2{\%} to 52.0 ± 1.2{\%} (p< 0.05) after 24. h. BMSCs had significant up regulation of the cartilage genes for both the labeled and the unlabeled samples compared to ADSCs (p< 0.05). PKH26 fluorescence was detected on the resected portions of the regenerated neo-cartilage. The recommended concentration of PKH26 for ADSCs is 2 μmol and BMSCs is 8 μmol, and they can be tracked up to 49 days.",
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AU - Chen, H. C.

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AU - Idrus, Ruszymah

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