Black cumin seed (Nigella sativa linn.) oil and its fractions protect against beta amyloid peptide-induced toxicity in primary cerebellar granule neurons

Norsharina Ismail, Maznah Ismail, Latiffah A. Latiff, Musalmah Mazlan, Abdalbasit A. Mariod

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    The neuroprotective effect of Nigella sativa oil (NSO) and its fractions against beta amyloid (Aβ)-induced cell death in primary rat cerebellar granule neurons was investigated. Primary cultures were pretreated for 5 h with NSO and its fractions - hexane fraction (HF), ethyl acetate fraction (EAF) and water fraction (WF) - before incubating with 10 μM Aβ1-40 for 24 h. Cell viability was investigated using the 3-(4,5-dimethylthiazol-2-yl)-5- (3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) and lactate dehydrogenase (LDH) assays. Results of the MTS assay showed that the WF and NSO were significantly protective against cell death induced by 10 μM Aβ1-40 at 1, 10 and 100 μg/mL probably because of antioxidant properties as determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical trapping method. HF and EAF had low DPPH scavenging effect and were only effective at 100 μg/mL. However, NSO and its fractions were weakly protective against cell membrane damage in the LDH assay. NSO and its fractions, especially the WF, may play a role in the prevention of Aβ-induced cell death.

    Original languageEnglish
    Pages (from-to)519-533
    Number of pages15
    JournalJournal of Food Lipids
    Volume15
    Issue number4
    DOIs
    Publication statusPublished - Nov 2008

    Fingerprint

    Nigella sativa
    Amyloid beta-Peptides
    amyloid
    Neurons
    Toxicity
    Seed
    granules
    Seeds
    Oils
    neurons
    peptides
    toxicity
    Cell death
    oils
    cell death
    Assays
    seeds
    Cell Death
    Hexanes
    lactate dehydrogenase

    ASJC Scopus subject areas

    • Food Science
    • Chemistry(all)

    Cite this

    Black cumin seed (Nigella sativa linn.) oil and its fractions protect against beta amyloid peptide-induced toxicity in primary cerebellar granule neurons. / Ismail, Norsharina; Ismail, Maznah; Latiff, Latiffah A.; Mazlan, Musalmah; Mariod, Abdalbasit A.

    In: Journal of Food Lipids, Vol. 15, No. 4, 11.2008, p. 519-533.

    Research output: Contribution to journalArticle

    Ismail, Norsharina ; Ismail, Maznah ; Latiff, Latiffah A. ; Mazlan, Musalmah ; Mariod, Abdalbasit A. / Black cumin seed (Nigella sativa linn.) oil and its fractions protect against beta amyloid peptide-induced toxicity in primary cerebellar granule neurons. In: Journal of Food Lipids. 2008 ; Vol. 15, No. 4. pp. 519-533.
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