Biosynthesis of very long polyunsaturated Omega-3 and Omega-6 fatty acids in transgenic Japonica rice (Oryza sativa L)

Tead Weng Cheah, Ismanizan Ismail, Nik Marzuki Sidek, Alina Wagiran, Ruslan Abdullah

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this research, the fatty acid pathway was modified to produce the C20 polyunsaturated fatty acid arachidonic acid (ARA) and eicosapentaenoic acid (EPA) by co-expression the genes encoding omega-3 fatty acid desaturase (SK-FAD3) from Saccharomyces kluyveri, δ9-elongase (IgASE1) from Isochrysis galbana, δ8-desaturase (efd1) from Euglena gracilis and δ5-desaturase from Mortierella alpina (Mortδ5) in japonica rice plant through Agrobacterium tumafaciens transformation method. The Southern hybridization analysis has confirmed the integration of these four transgenes and showed the presence of one copy number for each transgene in all five lines of T0 transgenic rice (T0-1, T0-2, T0-3, T0-4 and T0-5). The GC analysis showed the presence of new fatty acids eicosadienoic acid (EDA), eicosatrienoic acid (ETrA) and arachidonic (ARA) in transformed leaves of T0 and T1 compare to wild type. Further analysis of total fatty acid content in seeds showed that linolenic acids (LA) was increased at 1-2% in T0-2, T0-4 and T0-5 while a-linolenic acid (ALA) content accumulated up to 0.2% in T0-4 of transformed plant.

Original languageEnglish
Pages (from-to)1227-1234
Number of pages8
JournalAustralian Journal of Crop Science
Volume7
Issue number9
Publication statusPublished - 2013

Fingerprint

omega-6 fatty acids
linolenic acid
transgenes
Oryza sativa
Mortierella alpina
genetically modified organisms
biosynthesis
fatty acids
rice
Euglena gracilis
Isochrysis galbana
stearoyl-CoA desaturase
Saccharomyces
eicosapentaenoic acid
arachidonic acid
Agrobacterium
omega-3 fatty acids
Southern blotting
polyunsaturated fatty acids
fatty acid composition

Keywords

  • Agrobacterium tumefaciens
  • omega-3
  • omega-6
  • Oryza sativa L.
  • Transgenic plant
  • VLCPUFA

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Biosynthesis of very long polyunsaturated Omega-3 and Omega-6 fatty acids in transgenic Japonica rice (Oryza sativa L). / Cheah, Tead Weng; Ismail, Ismanizan; Sidek, Nik Marzuki; Wagiran, Alina; Abdullah, Ruslan.

In: Australian Journal of Crop Science, Vol. 7, No. 9, 2013, p. 1227-1234.

Research output: Contribution to journalArticle

Cheah, Tead Weng ; Ismail, Ismanizan ; Sidek, Nik Marzuki ; Wagiran, Alina ; Abdullah, Ruslan. / Biosynthesis of very long polyunsaturated Omega-3 and Omega-6 fatty acids in transgenic Japonica rice (Oryza sativa L). In: Australian Journal of Crop Science. 2013 ; Vol. 7, No. 9. pp. 1227-1234.
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