Biosurfactant production by the hydrocarbon-degrading bacteria (HDB) Serratia marcescens

Optimization using central composite design (CCD)

Asia Fadhile Almansoory, Hassimi Abu Hasan, Mushrifah Idris, Siti Rozaimah Sheikh Abdullah, Nurina Anuar, El Mubarak Musa Tibin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Based on the nutritional and physiological requirements of a native hydrocarbon-degrading bacterium (HDB), Serratia marcescens, an investigation on the potential biosurfactant production by this bacterial species was conducted. The effects of different carbon sources, nitrogen sources, salinity, pH, temperature, and agitation speeds were extensively studied. The optimal conditions of biosurfactant production and surface tension were determined using central composite design (CCD) by setting glycerol, peptone and ammonium sulphate (NH4)2SO4 in a range of 3-7%, 2-6g/L and 3-7g/L, respectively. The results showed that biosurfactant was highly produced at pH 8, a temperature of 30°C, a salinity of 1% and a speed of 200rpm after 5days (120h) of incubation. The optimal conditions were obtained at 5% glycerol, 4g/L peptone and 5g/L (NH4)2SO4 with a maximum biosurfactant production of 1.42g/L and a minimum surface tension of 28.4mN/m. Thus, the HDB S. marcescens shows good potential as a biosurfactant-producing bacterium to be used in any environmental application and as an alternative to chemical surfactants.

Original languageEnglish
JournalJournal of Industrial and Engineering Chemistry
DOIs
Publication statusAccepted/In press - 3 May 2016

Fingerprint

Hydrocarbons
Bacteria
Peptones
Glycerol
Surface tension
Composite materials
Ammonium Sulfate
Surface-Active Agents
Surface active agents
Nitrogen
Carbon
Temperature

Keywords

  • Serratia marcescens
  • Biosurfactant
  • Biosurfactant-producing bacteria
  • Hydrocarbon-degrading bacteria
  • Response surface methodology

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

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title = "Biosurfactant production by the hydrocarbon-degrading bacteria (HDB) Serratia marcescens: Optimization using central composite design (CCD)",
abstract = "Based on the nutritional and physiological requirements of a native hydrocarbon-degrading bacterium (HDB), Serratia marcescens, an investigation on the potential biosurfactant production by this bacterial species was conducted. The effects of different carbon sources, nitrogen sources, salinity, pH, temperature, and agitation speeds were extensively studied. The optimal conditions of biosurfactant production and surface tension were determined using central composite design (CCD) by setting glycerol, peptone and ammonium sulphate (NH4)2SO4 in a range of 3-7{\%}, 2-6g/L and 3-7g/L, respectively. The results showed that biosurfactant was highly produced at pH 8, a temperature of 30°C, a salinity of 1{\%} and a speed of 200rpm after 5days (120h) of incubation. The optimal conditions were obtained at 5{\%} glycerol, 4g/L peptone and 5g/L (NH4)2SO4 with a maximum biosurfactant production of 1.42g/L and a minimum surface tension of 28.4mN/m. Thus, the HDB S. marcescens shows good potential as a biosurfactant-producing bacterium to be used in any environmental application and as an alternative to chemical surfactants.",
keywords = "Serratia marcescens, Biosurfactant, Biosurfactant-producing bacteria, Hydrocarbon-degrading bacteria, Response surface methodology",
author = "{Fadhile Almansoory}, Asia and {Abu Hasan}, Hassimi and Mushrifah Idris and {Sheikh Abdullah}, {Siti Rozaimah} and Nurina Anuar and {Musa Tibin}, {El Mubarak}",
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T2 - Optimization using central composite design (CCD)

AU - Fadhile Almansoory, Asia

AU - Abu Hasan, Hassimi

AU - Idris, Mushrifah

AU - Sheikh Abdullah, Siti Rozaimah

AU - Anuar, Nurina

AU - Musa Tibin, El Mubarak

PY - 2016/5/3

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AB - Based on the nutritional and physiological requirements of a native hydrocarbon-degrading bacterium (HDB), Serratia marcescens, an investigation on the potential biosurfactant production by this bacterial species was conducted. The effects of different carbon sources, nitrogen sources, salinity, pH, temperature, and agitation speeds were extensively studied. The optimal conditions of biosurfactant production and surface tension were determined using central composite design (CCD) by setting glycerol, peptone and ammonium sulphate (NH4)2SO4 in a range of 3-7%, 2-6g/L and 3-7g/L, respectively. The results showed that biosurfactant was highly produced at pH 8, a temperature of 30°C, a salinity of 1% and a speed of 200rpm after 5days (120h) of incubation. The optimal conditions were obtained at 5% glycerol, 4g/L peptone and 5g/L (NH4)2SO4 with a maximum biosurfactant production of 1.42g/L and a minimum surface tension of 28.4mN/m. Thus, the HDB S. marcescens shows good potential as a biosurfactant-producing bacterium to be used in any environmental application and as an alternative to chemical surfactants.

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