Biosurfactant produced by the hydrocarbon-degrading bacteria

Characterization, activity and applications in removing TPH from contaminated soil

Asia Fadhile Almansoory, Hassimi Abu Hasan, Siti Rozaimah Sheikh Abdullah, Mushrifah Idris, Nurina Anuar, Wedad M. Al-Adiwish

Research output: Contribution to journalArticle

Abstract

In this study, the activity of the biosurfactant produced by the hydrocarbon-degrading bacteria (HDB) Serratia marcescens was investigated at various temperatures (30–100 °C), pH values (pH 4–10) and salt concentrations (1%–9%). The characterization of crude biosurfactant was performed using Fourier transform infrared spectroscopy (FTIR), thin-layer chromatography and tandem mass spectrometry, while purification was conducted by silica gel adsorption chromatography. The biosurfactant displayed good activity at a high temperature of 70 °C, a pH 7 and a salt concentration of 3%. FTIR identified hydroxyl, acetyl, carbonyl and aliphatic chain chemical functional groups. The purification of the crude biosurfactant resulted in three distinct fractions, i.e., glucose, 1-tetradecyl D-glucuronate (novel biosurfactant fraction) and lipids. The crude biosurfactant produced by the HDB S. marcescens is a novel, promising class of biosurfactant for the removal total petroleum hydrocarbon from contaminated soil. Based on these results, the biosurfactant may be an attractive choice for the oil industry for the decontamination of polluted sludge.

Original languageEnglish
Article number100347
JournalEnvironmental Technology and Innovation
Volume14
DOIs
Publication statusPublished - 1 May 2019

Fingerprint

biosurfactants
FTIR spectroscopy
polluted soils
chromatography
purification
hydrocarbons
hydrocarbon
salt
bacterium
bacteria
petroleum hydrocarbon
oil industry
functional group
glucose
gel
mass spectrometry
silica
lipid
sludge
adsorption

Keywords

  • Contaminated soil
  • Hydrocarbon-degrading bacteria
  • Phytoremediation
  • Serratia marcescens
  • Tetradecyl D-glucuronate biosurfactant

ASJC Scopus subject areas

  • Environmental Science(all)
  • Soil Science
  • Plant Science

Cite this

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title = "Biosurfactant produced by the hydrocarbon-degrading bacteria: Characterization, activity and applications in removing TPH from contaminated soil",
abstract = "In this study, the activity of the biosurfactant produced by the hydrocarbon-degrading bacteria (HDB) Serratia marcescens was investigated at various temperatures (30–100 °C), pH values (pH 4–10) and salt concentrations (1{\%}–9{\%}). The characterization of crude biosurfactant was performed using Fourier transform infrared spectroscopy (FTIR), thin-layer chromatography and tandem mass spectrometry, while purification was conducted by silica gel adsorption chromatography. The biosurfactant displayed good activity at a high temperature of 70 °C, a pH 7 and a salt concentration of 3{\%}. FTIR identified hydroxyl, acetyl, carbonyl and aliphatic chain chemical functional groups. The purification of the crude biosurfactant resulted in three distinct fractions, i.e., glucose, 1-tetradecyl D-glucuronate (novel biosurfactant fraction) and lipids. The crude biosurfactant produced by the HDB S. marcescens is a novel, promising class of biosurfactant for the removal total petroleum hydrocarbon from contaminated soil. Based on these results, the biosurfactant may be an attractive choice for the oil industry for the decontamination of polluted sludge.",
keywords = "Contaminated soil, Hydrocarbon-degrading bacteria, Phytoremediation, Serratia marcescens, Tetradecyl D-glucuronate biosurfactant",
author = "Almansoory, {Asia Fadhile} and {Abu Hasan}, Hassimi and {Sheikh Abdullah}, {Siti Rozaimah} and Mushrifah Idris and Nurina Anuar and Al-Adiwish, {Wedad M.}",
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language = "English",
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AU - Almansoory, Asia Fadhile

AU - Abu Hasan, Hassimi

AU - Sheikh Abdullah, Siti Rozaimah

AU - Idris, Mushrifah

AU - Anuar, Nurina

AU - Al-Adiwish, Wedad M.

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