Biosensor based on glutamate dehydrogenase immobilized in chitosan for the determination of ammonium in water samples

Nur Ellina Azmi, Musa Ahmad, Jaafar Abdullah, Hamidah Sidek, Yook Heng Lee, Nadarajah Karuppiah

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

An optical biosensor based on glutamate dehydrogenase (GLDH) immobilized in a chitosan film for the determination of ammonium in water samples is described. The biosensor film was deposited on a glass slide via a spin-coating method. The ammonium was measured based on β-nicotinamide adenine dinucleotide (NADH) oxidation in the presence of α-ketoglutaric acid at a wavelength of 340 nm. The biosensor showed optimum activity at pH 8. The optimum chitosan concentrations and enzyme loading were found to be at 2% (w/v) and 0.08 mg, respectively. Optimum concentrations of NADH and α-ketoglutaric acid both were obtained at 0.15 mM. A linear response of the biosensor was obtained in the ammonium concentration range of 0.005 to 0.5 mM with a detection limit of 0.005 mM. The reproducibility of the biosensor was good, with an observed relative standard deviation of 5.9% (n = 8). The biosensor was found to be stable for at least 1 month when stored dry at 4 °C.

Original languageEnglish
Pages (from-to)28-32
Number of pages5
JournalAnalytical Biochemistry
Volume388
Issue number1
DOIs
Publication statusPublished - 1 May 2009

Fingerprint

Glutamate Dehydrogenase
Chitosan
Biosensing Techniques
Ammonium Compounds
Biosensors
Water
NAD
Ketoglutaric Acids
Spin coating
Glass
Limit of Detection
Wavelength
Oxidation
Enzymes

Keywords

  • Ammonium
  • Chitosan
  • Glutamate dehydrogenase
  • Optical biosensor

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Biosensor based on glutamate dehydrogenase immobilized in chitosan for the determination of ammonium in water samples. / Azmi, Nur Ellina; Ahmad, Musa; Abdullah, Jaafar; Sidek, Hamidah; Lee, Yook Heng; Karuppiah, Nadarajah.

In: Analytical Biochemistry, Vol. 388, No. 1, 01.05.2009, p. 28-32.

Research output: Contribution to journalArticle

Azmi, Nur Ellina ; Ahmad, Musa ; Abdullah, Jaafar ; Sidek, Hamidah ; Lee, Yook Heng ; Karuppiah, Nadarajah. / Biosensor based on glutamate dehydrogenase immobilized in chitosan for the determination of ammonium in water samples. In: Analytical Biochemistry. 2009 ; Vol. 388, No. 1. pp. 28-32.
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