Biomarkers of neutrophil-mediated glutathione and protein oxidation in tracheal aspirates from preterm infants: Association with bacterial infection

D. Tim Harwood, Brian A. Darlow, Cheah Fook Choe, Nicolette McNeill, Patricia Graham, Christine C. Winterbourn

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Bronchopulmonary dysplasia is associated with neutrophil infiltration into the lungs and oxidative injury. However, the pathological importance of neutrophil oxidants is still not clear. Nosocomial pneumonia is also implicated, but the evidence is limited, in part because of the difficulty of distinguishing genuine infection from bacterial colonization. Good biomarkers of neutrophil oxidant activity and lung infection are needed. We tested whether glutathione sulfonamide, a product of glutathione oxidation by myeloperoxidase-derived hypochlorous acid (HOCl) and a potential new neutrophil oxidant biomarker, is detectable in endotracheal aspirates from ventilated preterm infants. As infectious organisms stimulate neutrophils to generate HOCl, we determined whether levels of HOCl-specific biomarkers were increased in samples that were bacterial culture positive. Glutathione sulfonamide was detected in 66 of 87 endotracheal aspirate samples. Levels correlated with myeloperoxidase activity and another HOCl-specific marker, chlorotyrosine. Median levels of glutathione sulfonamide (4-fold) and other biomarkers (2-fold) were significantly higher in culture positive aspirates. Staphylococcus epidermidis, a frequent colonizer, was associated with glutathione sulfonamide levels no different from those in negative samples. Glutathione sulfonamide showed good sensitivity and specificity for detecting bacterial growth and has promise for detecting lung infection.

Original languageEnglish
Pages (from-to)28-33
Number of pages6
JournalPediatric Research
Volume69
Issue number1
DOIs
Publication statusPublished - Jan 2011

Fingerprint

Hypochlorous Acid
Bacterial Infections
Premature Infants
Glutathione
Neutrophils
Biomarkers
Oxidants
Proteins
Peroxidase
Bronchopulmonary Dysplasia
Lung
Staphylococcus epidermidis
Neutrophil Infiltration
Lung Injury
Infection
Pneumonia
glutathione sulfonamide
Sensitivity and Specificity
Growth

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Biomarkers of neutrophil-mediated glutathione and protein oxidation in tracheal aspirates from preterm infants : Association with bacterial infection. / Harwood, D. Tim; Darlow, Brian A.; Fook Choe, Cheah; McNeill, Nicolette; Graham, Patricia; Winterbourn, Christine C.

In: Pediatric Research, Vol. 69, No. 1, 01.2011, p. 28-33.

Research output: Contribution to journalArticle

Harwood, D. Tim ; Darlow, Brian A. ; Fook Choe, Cheah ; McNeill, Nicolette ; Graham, Patricia ; Winterbourn, Christine C. / Biomarkers of neutrophil-mediated glutathione and protein oxidation in tracheal aspirates from preterm infants : Association with bacterial infection. In: Pediatric Research. 2011 ; Vol. 69, No. 1. pp. 28-33.
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