Biological activities of isolated compounds from three edible Malaysian red seaweeds, gracilaria changii, G. manilaensis and Gracilaria sp.

Yosie Andriani, Desy Fitrya Syamsumir, Tee Ching Yee, Faizah Shaharom Harisson, Gan Ming Herng, Siti Aishah Abdullah, Christine Abellana Orosco, Abdul Manaf Ali, Jalifah Latip, Hiroe Kikuzaki, Habsah Mohamad

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Gracilaria species are red marine macroalgae that are found abundantly in Malaysia. Gracilaria changii from Morib, Selangor, G. manilaensis and Gracilaria sp. from Gelang Patah, Johor were used in this study. Five compounds were successfully isolated and identified as hexadecanoic acid (1), cholest-5-en-3-ol (2), 2-hydroxymyristic acid (3), cholesteryl myristate (4) and 1-(4'-methoxyphenyl)-3-(2,4,6-trihydroxyphenyl)-3-hydroxypropanone (5) based on spectral data analysis (IR, UV, GC-MS, 1H NMR, 13C NMR, HMQC and HMBC). All compounds isolated were tested for cytotoxicity (MTT assay for HL-60 and MCF-7 cell lines), and antibacterial (disc diffusion method), antioxidant (DPPH free radical scavenging assay and xanthine oxidase inhibitory assay) and acetylcholinesterase inhibitory (AChE) activity (TLC bioautographic method). Compounds 1 and 3 exhibited strong cytotoxic activity against HL-60 and MCF-7 cell lines. Compound 5 showed high antioxidant activity in both the DPPH free radical scavenging and xanthine oxidase inhibition assays. Compound 1 showed positive activity for AChE inhibitory with a minimum inhibition dose of 0.625 ?g sample. All compounds demonstrated antibacterial activity producing 8 to 14 mm inhibition zones. A positive control was applied to all bioassays and experiments were performed with three replicates. Results demonstrated that three edible red seaweeds are rich sources of bioactive compounds with potential application for pharmaceutical purposes.

Original languageEnglish
Pages (from-to)1117-1120
Number of pages4
JournalNatural Product Communications
Volume11
Issue number8
Publication statusPublished - 2016

Fingerprint

Gracilaria
Seaweed
macroalgae
bioactive properties
Xanthine Oxidase
MCF-7 Cells
Acetylcholinesterase
Free Radicals
xanthine oxidase
assays
acetylcholinesterase
Antioxidants
Cell Line
Palmitic Acid
Malaysia
cell lines
Biological Assay
palmitic acid
spectral analysis
cytotoxicity

Keywords

  • 1-(4'-Methoxyphenyl)-3-(2,4,6-trihydroxyphenyl)-3-hydroxypropanone
  • Acetylcholinesterase (AChE) inhibitors
  • Antibacterial
  • Antioxidant
  • Cytotoxic
  • Gracilaria

ASJC Scopus subject areas

  • Medicine(all)
  • Pharmacology
  • Plant Science
  • Drug Discovery
  • Complementary and alternative medicine

Cite this

Andriani, Y., Syamsumir, D. F., Yee, T. C., Harisson, F. S., Herng, G. M., Abdullah, S. A., ... Mohamad, H. (2016). Biological activities of isolated compounds from three edible Malaysian red seaweeds, gracilaria changii, G. manilaensis and Gracilaria sp. Natural Product Communications, 11(8), 1117-1120.

Biological activities of isolated compounds from three edible Malaysian red seaweeds, gracilaria changii, G. manilaensis and Gracilaria sp. / Andriani, Yosie; Syamsumir, Desy Fitrya; Yee, Tee Ching; Harisson, Faizah Shaharom; Herng, Gan Ming; Abdullah, Siti Aishah; Orosco, Christine Abellana; Ali, Abdul Manaf; Latip, Jalifah; Kikuzaki, Hiroe; Mohamad, Habsah.

In: Natural Product Communications, Vol. 11, No. 8, 2016, p. 1117-1120.

Research output: Contribution to journalArticle

Andriani, Y, Syamsumir, DF, Yee, TC, Harisson, FS, Herng, GM, Abdullah, SA, Orosco, CA, Ali, AM, Latip, J, Kikuzaki, H & Mohamad, H 2016, 'Biological activities of isolated compounds from three edible Malaysian red seaweeds, gracilaria changii, G. manilaensis and Gracilaria sp.', Natural Product Communications, vol. 11, no. 8, pp. 1117-1120.
Andriani, Yosie ; Syamsumir, Desy Fitrya ; Yee, Tee Ching ; Harisson, Faizah Shaharom ; Herng, Gan Ming ; Abdullah, Siti Aishah ; Orosco, Christine Abellana ; Ali, Abdul Manaf ; Latip, Jalifah ; Kikuzaki, Hiroe ; Mohamad, Habsah. / Biological activities of isolated compounds from three edible Malaysian red seaweeds, gracilaria changii, G. manilaensis and Gracilaria sp. In: Natural Product Communications. 2016 ; Vol. 11, No. 8. pp. 1117-1120.
@article{16d50cbd64e1496fa47af398ad63f40b,
title = "Biological activities of isolated compounds from three edible Malaysian red seaweeds, gracilaria changii, G. manilaensis and Gracilaria sp.",
abstract = "Gracilaria species are red marine macroalgae that are found abundantly in Malaysia. Gracilaria changii from Morib, Selangor, G. manilaensis and Gracilaria sp. from Gelang Patah, Johor were used in this study. Five compounds were successfully isolated and identified as hexadecanoic acid (1), cholest-5-en-3-ol (2), 2-hydroxymyristic acid (3), cholesteryl myristate (4) and 1-(4'-methoxyphenyl)-3-(2,4,6-trihydroxyphenyl)-3-hydroxypropanone (5) based on spectral data analysis (IR, UV, GC-MS, 1H NMR, 13C NMR, HMQC and HMBC). All compounds isolated were tested for cytotoxicity (MTT assay for HL-60 and MCF-7 cell lines), and antibacterial (disc diffusion method), antioxidant (DPPH free radical scavenging assay and xanthine oxidase inhibitory assay) and acetylcholinesterase inhibitory (AChE) activity (TLC bioautographic method). Compounds 1 and 3 exhibited strong cytotoxic activity against HL-60 and MCF-7 cell lines. Compound 5 showed high antioxidant activity in both the DPPH free radical scavenging and xanthine oxidase inhibition assays. Compound 1 showed positive activity for AChE inhibitory with a minimum inhibition dose of 0.625 ?g sample. All compounds demonstrated antibacterial activity producing 8 to 14 mm inhibition zones. A positive control was applied to all bioassays and experiments were performed with three replicates. Results demonstrated that three edible red seaweeds are rich sources of bioactive compounds with potential application for pharmaceutical purposes.",
keywords = "1-(4'-Methoxyphenyl)-3-(2,4,6-trihydroxyphenyl)-3-hydroxypropanone, Acetylcholinesterase (AChE) inhibitors, Antibacterial, Antioxidant, Cytotoxic, Gracilaria",
author = "Yosie Andriani and Syamsumir, {Desy Fitrya} and Yee, {Tee Ching} and Harisson, {Faizah Shaharom} and Herng, {Gan Ming} and Abdullah, {Siti Aishah} and Orosco, {Christine Abellana} and Ali, {Abdul Manaf} and Jalifah Latip and Hiroe Kikuzaki and Habsah Mohamad",
year = "2016",
language = "English",
volume = "11",
pages = "1117--1120",
journal = "Natural Product Communications",
issn = "1934-578X",
publisher = "Natural Product Communications",
number = "8",

}

TY - JOUR

T1 - Biological activities of isolated compounds from three edible Malaysian red seaweeds, gracilaria changii, G. manilaensis and Gracilaria sp.

AU - Andriani, Yosie

AU - Syamsumir, Desy Fitrya

AU - Yee, Tee Ching

AU - Harisson, Faizah Shaharom

AU - Herng, Gan Ming

AU - Abdullah, Siti Aishah

AU - Orosco, Christine Abellana

AU - Ali, Abdul Manaf

AU - Latip, Jalifah

AU - Kikuzaki, Hiroe

AU - Mohamad, Habsah

PY - 2016

Y1 - 2016

N2 - Gracilaria species are red marine macroalgae that are found abundantly in Malaysia. Gracilaria changii from Morib, Selangor, G. manilaensis and Gracilaria sp. from Gelang Patah, Johor were used in this study. Five compounds were successfully isolated and identified as hexadecanoic acid (1), cholest-5-en-3-ol (2), 2-hydroxymyristic acid (3), cholesteryl myristate (4) and 1-(4'-methoxyphenyl)-3-(2,4,6-trihydroxyphenyl)-3-hydroxypropanone (5) based on spectral data analysis (IR, UV, GC-MS, 1H NMR, 13C NMR, HMQC and HMBC). All compounds isolated were tested for cytotoxicity (MTT assay for HL-60 and MCF-7 cell lines), and antibacterial (disc diffusion method), antioxidant (DPPH free radical scavenging assay and xanthine oxidase inhibitory assay) and acetylcholinesterase inhibitory (AChE) activity (TLC bioautographic method). Compounds 1 and 3 exhibited strong cytotoxic activity against HL-60 and MCF-7 cell lines. Compound 5 showed high antioxidant activity in both the DPPH free radical scavenging and xanthine oxidase inhibition assays. Compound 1 showed positive activity for AChE inhibitory with a minimum inhibition dose of 0.625 ?g sample. All compounds demonstrated antibacterial activity producing 8 to 14 mm inhibition zones. A positive control was applied to all bioassays and experiments were performed with three replicates. Results demonstrated that three edible red seaweeds are rich sources of bioactive compounds with potential application for pharmaceutical purposes.

AB - Gracilaria species are red marine macroalgae that are found abundantly in Malaysia. Gracilaria changii from Morib, Selangor, G. manilaensis and Gracilaria sp. from Gelang Patah, Johor were used in this study. Five compounds were successfully isolated and identified as hexadecanoic acid (1), cholest-5-en-3-ol (2), 2-hydroxymyristic acid (3), cholesteryl myristate (4) and 1-(4'-methoxyphenyl)-3-(2,4,6-trihydroxyphenyl)-3-hydroxypropanone (5) based on spectral data analysis (IR, UV, GC-MS, 1H NMR, 13C NMR, HMQC and HMBC). All compounds isolated were tested for cytotoxicity (MTT assay for HL-60 and MCF-7 cell lines), and antibacterial (disc diffusion method), antioxidant (DPPH free radical scavenging assay and xanthine oxidase inhibitory assay) and acetylcholinesterase inhibitory (AChE) activity (TLC bioautographic method). Compounds 1 and 3 exhibited strong cytotoxic activity against HL-60 and MCF-7 cell lines. Compound 5 showed high antioxidant activity in both the DPPH free radical scavenging and xanthine oxidase inhibition assays. Compound 1 showed positive activity for AChE inhibitory with a minimum inhibition dose of 0.625 ?g sample. All compounds demonstrated antibacterial activity producing 8 to 14 mm inhibition zones. A positive control was applied to all bioassays and experiments were performed with three replicates. Results demonstrated that three edible red seaweeds are rich sources of bioactive compounds with potential application for pharmaceutical purposes.

KW - 1-(4'-Methoxyphenyl)-3-(2,4,6-trihydroxyphenyl)-3-hydroxypropanone

KW - Acetylcholinesterase (AChE) inhibitors

KW - Antibacterial

KW - Antioxidant

KW - Cytotoxic

KW - Gracilaria

UR - http://www.scopus.com/inward/record.url?scp=85019059717&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019059717&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:85019059717

VL - 11

SP - 1117

EP - 1120

JO - Natural Product Communications

JF - Natural Product Communications

SN - 1934-578X

IS - 8

ER -