Biohydrogen production from ricebran using Clostridium saccharoperbutylacetonicum N1-4

Olujimi Dada, Wan Mohtar Wan Yusoff, Mohd. Sahaid Kalil

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Treated ricebran hydrolysate was fermented anaerobically using Clostridium saccharoperbutylacetonicum N1-4 at an initial pH of 6 ± 0.2 and an operating temperature of 30 C for production of hydrogen. The effects of different pretreatment methods on the liberation of sugar from 100 g of ricebran per litre of medium (distilled water) were investigated. In addition, the effects of the pretreatment method on ricebran hydrolysates of different initial ricebran concentrations on liberated sugar as well as the effects of the initial inoculum concentration, ricebran (substrate) concentration, and FeSO4·7H2O concentration on the yield as well as the productivity of hydrogen were investigated. The combination of enzymatic hydrolysis and a boiling pretreatment method produced the most fermentable sugar, 29.03 ± 0.0 g/L from 100 g of ricebran per litre of medium (distilled water), while the amount of sugar liberated by ricebran hydrolysates of different initial ricebran concentrations upon pretreatment monotonically increased with the initial ricebran concentration. The increment in substrate, inoculum, and FeSO4·7H2O concentrations had a significantly positive effect (p < 0.05) on both the yield and productivity of hydrogen. The maximum hydrogen gas yield (YP/S) and productivity of 3.37 mol-H2 per mol-sugar consumed and 7.58 mmol/(L h), respectively, were obtained from ricebran hydrolysate with a 100 g/L ricebran concentration (equivalent to 28.59 ± 1.27 g sugar/L). In other experiments, 0.03 g/L FeSO4·7H2O and 1.5 g/L inoculum resulted in the best hydrogen gas yield and productivity from ricebran hydrolysates.

Original languageEnglish
Pages (from-to)15063-15073
Number of pages11
JournalInternational Journal of Hydrogen Energy
Volume38
Issue number35
DOIs
Publication statusPublished - 22 Nov 2013

Fingerprint

Clostridium
Sugars
sugars
inoculum
Hydrogen
Productivity
productivity
pretreatment
hydrogen
Enzymatic hydrolysis
Substrates
Gases
Boiling liquids
Water
operating temperature
gases
boiling
water
hydrolysis

Keywords

  • Biohydrogen
  • Clostridium saccharoperbutylacetonicum N1-4
  • FeSO ·7HO concentration
  • Ricebran

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

Biohydrogen production from ricebran using Clostridium saccharoperbutylacetonicum N1-4. / Dada, Olujimi; Wan Yusoff, Wan Mohtar; Kalil, Mohd. Sahaid.

In: International Journal of Hydrogen Energy, Vol. 38, No. 35, 22.11.2013, p. 15063-15073.

Research output: Contribution to journalArticle

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