Biofilm forming ability of bacteria isolated from necrotic roots canals of teeth

Merriam Ghadhanfar Alwan, Gires Usup, Yook Heng Lee, Asmat Ahmad

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The growth of microbes in biofilms are associated with repeated and chronic human infections and are extremely resistant to antimicrobial agents. The purpose of this study was to determine the diversity of bacteria from necrotic roots canals of teeth and to detect their biofilm formation ability. A total of 42 bacterial isolates were isolated and identified as belonging to 11 genera. These are Enterococcus sp. (21.4%) followed by Streptococcus sp. (16.8%), Bacillus sp. (11.9%), Peptostreptococcus sp. (9.5%), Staphylococcus sp. (9.5%), Bacteroides sp. (7.1%), Clostridium sp. (7.1%), Actinomyces sp. (7.1%), Fusobacterium sp. (4.76%), Provotella sp. (2.4%) and Chromobacterium sp. (2.4%). Three screening methods for biofilm forming ability were used. Congo Red Agar method (CRA), Tube method (TM) and Microtitre Plate (MTP). From the results, MTP method is a more reliable and quantitative method for the screening and detection of microorganism's ability to form biofilm. This method can be recommended and suggested as a general screening method for the detection of biofilm forming bacteria isolated from roots canals of teeth.

Original languageEnglish
Title of host publication2017 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1940
ISBN (Electronic)9780735416321
DOIs
Publication statusPublished - 4 Apr 2018
Event2017 UKM FST Postgraduate Colloquium - Selangor, Malaysia
Duration: 12 Jul 201713 Jul 2017

Other

Other2017 UKM FST Postgraduate Colloquium
CountryMalaysia
CitySelangor
Period12/7/1713/7/17

Fingerprint

biofilms
canals
teeth
bacteria
screening
microorganisms
Clostridium
streptococcus
staphylococcus
Bacillus
infectious diseases
tubes

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Alwan, M. G., Usup, G., Lee, Y. H., & Ahmad, A. (2018). Biofilm forming ability of bacteria isolated from necrotic roots canals of teeth. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium (Vol. 1940). [020078] American Institute of Physics Inc.. https://doi.org/10.1063/1.5027993

Biofilm forming ability of bacteria isolated from necrotic roots canals of teeth. / Alwan, Merriam Ghadhanfar; Usup, Gires; Lee, Yook Heng; Ahmad, Asmat.

2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018. 020078.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Alwan, MG, Usup, G, Lee, YH & Ahmad, A 2018, Biofilm forming ability of bacteria isolated from necrotic roots canals of teeth. in 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. vol. 1940, 020078, American Institute of Physics Inc., 2017 UKM FST Postgraduate Colloquium, Selangor, Malaysia, 12/7/17. https://doi.org/10.1063/1.5027993
Alwan MG, Usup G, Lee YH, Ahmad A. Biofilm forming ability of bacteria isolated from necrotic roots canals of teeth. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940. American Institute of Physics Inc. 2018. 020078 https://doi.org/10.1063/1.5027993
Alwan, Merriam Ghadhanfar ; Usup, Gires ; Lee, Yook Heng ; Ahmad, Asmat. / Biofilm forming ability of bacteria isolated from necrotic roots canals of teeth. 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018.
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