Biofilm formation on granular activated carbon in xylose and glucose mixture for thermophilic biohydrogen production

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22 Citations (Scopus)

Abstract

Granular activated carbon (GAC) was used as a support carrier to develop biofilm of Thermophilic biohydrogen producer in an immobilized system of dark fermentation. The optimum ratio of the sludge to GAC loading was investigated in batch fermentation using glucose and xylose mixture as a carbon source. It was found that the highest hydrogen yield of 1.77 mol H2/mol substrate consumed and hydrogen production rate (HPR) of 2.0 mmol H2/l.h, were achieved at a sludge-GAC ratio of 1:2. On the other hand, the experiments with suspended culture as a control gave poor performance of hydrogen yield (0.86 mol H2/mol of substrate consumed) and HPR (0.5 mmol H2/l.h). The sludge-GAC biofilm was further developed in a sequencing batch feeding mode through controlled acclimatization condition. Stable hydrogen production was achieved after day 40th with consistent HPR of 2.4 mmol H2/l.h and yield of 1.17 mol H2/mol substrate consumed with 44.2% of hydrogen. Acetic and butyric acids dominate volatile fatty acids (TVFAs) as a major by-product while ethanol being the only alcohol produced but in a minor amount. This work has proven the possible future of GAC attached biofilm sludge as promising attachment system to achieve consistent hydrogen production even at thermophilic conditions.

Original languageEnglish
JournalInternational Journal of Hydrogen Energy
DOIs
Publication statusAccepted/In press - 21 Jan 2016

Fingerprint

xylose
biofilms
Xylose
Biofilms
hydrogen production
activated carbon
Hydrogen production
glucose
sludge
Activated carbon
Glucose
fermentation
Fermentation
Hydrogen
acclimatization
Substrates
hydrogen
Volatile fatty acids
Butyric acid
sequencing

Keywords

  • Batch processing
  • Biofilms
  • Biohydrogen
  • Fermentation
  • Granular activated carbon (GAC)
  • Thermophiles

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

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title = "Biofilm formation on granular activated carbon in xylose and glucose mixture for thermophilic biohydrogen production",
abstract = "Granular activated carbon (GAC) was used as a support carrier to develop biofilm of Thermophilic biohydrogen producer in an immobilized system of dark fermentation. The optimum ratio of the sludge to GAC loading was investigated in batch fermentation using glucose and xylose mixture as a carbon source. It was found that the highest hydrogen yield of 1.77 mol H2/mol substrate consumed and hydrogen production rate (HPR) of 2.0 mmol H2/l.h, were achieved at a sludge-GAC ratio of 1:2. On the other hand, the experiments with suspended culture as a control gave poor performance of hydrogen yield (0.86 mol H2/mol of substrate consumed) and HPR (0.5 mmol H2/l.h). The sludge-GAC biofilm was further developed in a sequencing batch feeding mode through controlled acclimatization condition. Stable hydrogen production was achieved after day 40th with consistent HPR of 2.4 mmol H2/l.h and yield of 1.17 mol H2/mol substrate consumed with 44.2{\%} of hydrogen. Acetic and butyric acids dominate volatile fatty acids (TVFAs) as a major by-product while ethanol being the only alcohol produced but in a minor amount. This work has proven the possible future of GAC attached biofilm sludge as promising attachment system to achieve consistent hydrogen production even at thermophilic conditions.",
keywords = "Batch processing, Biofilms, Biohydrogen, Fermentation, Granular activated carbon (GAC), Thermophiles",
author = "Jamali, {Nur Syakina} and {Md Jahim}, Jamaliah and {Wan Nor Roslam}, {Wan Isahak}",
year = "2016",
month = "1",
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doi = "10.1016/j.ijhydene.2016.05.092",
language = "English",
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T1 - Biofilm formation on granular activated carbon in xylose and glucose mixture for thermophilic biohydrogen production

AU - Jamali, Nur Syakina

AU - Md Jahim, Jamaliah

AU - Wan Nor Roslam, Wan Isahak

PY - 2016/1/21

Y1 - 2016/1/21

N2 - Granular activated carbon (GAC) was used as a support carrier to develop biofilm of Thermophilic biohydrogen producer in an immobilized system of dark fermentation. The optimum ratio of the sludge to GAC loading was investigated in batch fermentation using glucose and xylose mixture as a carbon source. It was found that the highest hydrogen yield of 1.77 mol H2/mol substrate consumed and hydrogen production rate (HPR) of 2.0 mmol H2/l.h, were achieved at a sludge-GAC ratio of 1:2. On the other hand, the experiments with suspended culture as a control gave poor performance of hydrogen yield (0.86 mol H2/mol of substrate consumed) and HPR (0.5 mmol H2/l.h). The sludge-GAC biofilm was further developed in a sequencing batch feeding mode through controlled acclimatization condition. Stable hydrogen production was achieved after day 40th with consistent HPR of 2.4 mmol H2/l.h and yield of 1.17 mol H2/mol substrate consumed with 44.2% of hydrogen. Acetic and butyric acids dominate volatile fatty acids (TVFAs) as a major by-product while ethanol being the only alcohol produced but in a minor amount. This work has proven the possible future of GAC attached biofilm sludge as promising attachment system to achieve consistent hydrogen production even at thermophilic conditions.

AB - Granular activated carbon (GAC) was used as a support carrier to develop biofilm of Thermophilic biohydrogen producer in an immobilized system of dark fermentation. The optimum ratio of the sludge to GAC loading was investigated in batch fermentation using glucose and xylose mixture as a carbon source. It was found that the highest hydrogen yield of 1.77 mol H2/mol substrate consumed and hydrogen production rate (HPR) of 2.0 mmol H2/l.h, were achieved at a sludge-GAC ratio of 1:2. On the other hand, the experiments with suspended culture as a control gave poor performance of hydrogen yield (0.86 mol H2/mol of substrate consumed) and HPR (0.5 mmol H2/l.h). The sludge-GAC biofilm was further developed in a sequencing batch feeding mode through controlled acclimatization condition. Stable hydrogen production was achieved after day 40th with consistent HPR of 2.4 mmol H2/l.h and yield of 1.17 mol H2/mol substrate consumed with 44.2% of hydrogen. Acetic and butyric acids dominate volatile fatty acids (TVFAs) as a major by-product while ethanol being the only alcohol produced but in a minor amount. This work has proven the possible future of GAC attached biofilm sludge as promising attachment system to achieve consistent hydrogen production even at thermophilic conditions.

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