Biodegradation of monochloroacetic acid by a presumptive Pseudomonas sp. strain R1 bacterium isolated from malaysian paddy (Rice) field

Siti Nurmadihah Ismail, Aishah Mohd Taha, Ng Hong Jing, Roswanira Ab Wahab, Aidil Abdul Hamid, Rolando V. Pakingking, Fahrul Huyop

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A bacterial strain tentatively identified as Pseudomonas sp. R1 was isolated from a paddy (rice) field that could degrade monochloroacetic acid (MCA) for concentrations ranging from 5 to 40 mM. Quantitative agreement between the amount of MCA introduced and chloride released was also found. MCA dehalogenase activity in this strain was found to be inducible. Cell-free extracts displayed dehalogenating activity with specific halogenated organic compound with no activity on dichloropropionic acid or monochloropropionic acid. The estimated Km values for MCA was 0.14 mM. The optimal pH range for MCA dehalogenase activity (between pH 6.5 and 8.0), whereas the thermal stability profile stable up to 50°C. The results of our current study demonstrated the potential use of Pseudomonas sp. R1 as suitable biological agent for biodegradation of MCA in contaminated agricultural area.

Original languageEnglish
Pages (from-to)481-486
Number of pages6
JournalBiotechnology
Volume7
Issue number3
DOIs
Publication statusPublished - 2008

Fingerprint

Pseudomonas
Biodegradation
Bacteria
Acids
Biological Factors
Cell Extracts
Organic compounds
chloroacetic acid
Oryza
Chlorides
Thermodynamic stability
Hot Temperature

Keywords

  • Biodegradation
  • Dehalogenase
  • Monochloroacetate
  • Pseudomonas sp

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Biodegradation of monochloroacetic acid by a presumptive Pseudomonas sp. strain R1 bacterium isolated from malaysian paddy (Rice) field. / Ismail, Siti Nurmadihah; Taha, Aishah Mohd; Jing, Ng Hong; Wahab, Roswanira Ab; Abdul Hamid, Aidil; Pakingking, Rolando V.; Huyop, Fahrul.

In: Biotechnology, Vol. 7, No. 3, 2008, p. 481-486.

Research output: Contribution to journalArticle

Ismail, Siti Nurmadihah ; Taha, Aishah Mohd ; Jing, Ng Hong ; Wahab, Roswanira Ab ; Abdul Hamid, Aidil ; Pakingking, Rolando V. ; Huyop, Fahrul. / Biodegradation of monochloroacetic acid by a presumptive Pseudomonas sp. strain R1 bacterium isolated from malaysian paddy (Rice) field. In: Biotechnology. 2008 ; Vol. 7, No. 3. pp. 481-486.
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