Abstract
Three bacterial isolates identified as Pseudomonas aeruginosa (UKMP-8T), Rhodococcus sp. M15-2 (UKMP-5T), and Rhodococcus sp. ZH8 (UKMP-7T) based on biochemical, physiological, and morphological characteristics and on 16S rDNA sequences were isolated from groundwater of a crude oil refinery plant. From these three isolates, four bacterial consortia were designed by mixing the single bacterial cultures in the following ratios: (P. aeruginosa:Rhodococcus sp. M15-2, 1:1), (P. aeruginosa:Rhodococcus sp. ZH8, 1:1), (Rhodococcus sp. M15-2: Rhodococcus sp. ZH8, 1:1), and (P. aeruginosa: Rhodococcus sp. ZH8:Rhodococcus sp. M15-2, 1:1:1), respectively. Bacterial isolates and consortia showed differing preferences for nitrogen source (0.01% ammonium chloride, 0.10% yeast extract, or 0.50% peptone) to reach optimum growth. When fortified with the preferred nitrogen sources and grown in minimal salt medium, within 7 days all three single isolates and the four bacterial consortia biodegraded 97.6-99.9% of Tapis Massa oil without any significant differences.
| Original language | English |
|---|---|
| Pages (from-to) | 1-10 |
| Number of pages | 10 |
| Journal | Bioremediation Journal |
| Volume | 17 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2013 |
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Keywords
- bacterial consortia
- biodegradation
- crude oil
- nitrogen sources
- Pseudomonas aeruginosa
- Rhodococcus sp.
ASJC Scopus subject areas
- Environmental Science(all)
Cite this
Biodegradation of crude oil by constructed bacterial consortia and the constituent single bacteria isolated from Malaysia. / Hamzah, Ainon; Phan, Chia Wei; Abu Bakar, Nur Faizah; Wong, Kok Kee.
In: Bioremediation Journal, Vol. 17, No. 1, 2013, p. 1-10.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biodegradation of crude oil by constructed bacterial consortia and the constituent single bacteria isolated from Malaysia
AU - Hamzah, Ainon
AU - Phan, Chia Wei
AU - Abu Bakar, Nur Faizah
AU - Wong, Kok Kee
PY - 2013
Y1 - 2013
N2 - Three bacterial isolates identified as Pseudomonas aeruginosa (UKMP-8T), Rhodococcus sp. M15-2 (UKMP-5T), and Rhodococcus sp. ZH8 (UKMP-7T) based on biochemical, physiological, and morphological characteristics and on 16S rDNA sequences were isolated from groundwater of a crude oil refinery plant. From these three isolates, four bacterial consortia were designed by mixing the single bacterial cultures in the following ratios: (P. aeruginosa:Rhodococcus sp. M15-2, 1:1), (P. aeruginosa:Rhodococcus sp. ZH8, 1:1), (Rhodococcus sp. M15-2: Rhodococcus sp. ZH8, 1:1), and (P. aeruginosa: Rhodococcus sp. ZH8:Rhodococcus sp. M15-2, 1:1:1), respectively. Bacterial isolates and consortia showed differing preferences for nitrogen source (0.01% ammonium chloride, 0.10% yeast extract, or 0.50% peptone) to reach optimum growth. When fortified with the preferred nitrogen sources and grown in minimal salt medium, within 7 days all three single isolates and the four bacterial consortia biodegraded 97.6-99.9% of Tapis Massa oil without any significant differences.
AB - Three bacterial isolates identified as Pseudomonas aeruginosa (UKMP-8T), Rhodococcus sp. M15-2 (UKMP-5T), and Rhodococcus sp. ZH8 (UKMP-7T) based on biochemical, physiological, and morphological characteristics and on 16S rDNA sequences were isolated from groundwater of a crude oil refinery plant. From these three isolates, four bacterial consortia were designed by mixing the single bacterial cultures in the following ratios: (P. aeruginosa:Rhodococcus sp. M15-2, 1:1), (P. aeruginosa:Rhodococcus sp. ZH8, 1:1), (Rhodococcus sp. M15-2: Rhodococcus sp. ZH8, 1:1), and (P. aeruginosa: Rhodococcus sp. ZH8:Rhodococcus sp. M15-2, 1:1:1), respectively. Bacterial isolates and consortia showed differing preferences for nitrogen source (0.01% ammonium chloride, 0.10% yeast extract, or 0.50% peptone) to reach optimum growth. When fortified with the preferred nitrogen sources and grown in minimal salt medium, within 7 days all three single isolates and the four bacterial consortia biodegraded 97.6-99.9% of Tapis Massa oil without any significant differences.
KW - bacterial consortia
KW - biodegradation
KW - crude oil
KW - nitrogen sources
KW - Pseudomonas aeruginosa
KW - Rhodococcus sp.
UR - http://www.scopus.com/inward/record.url?scp=84874612942&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84874612942&partnerID=8YFLogxK
U2 - 10.1080/10889868.2012.731447
DO - 10.1080/10889868.2012.731447
M3 - Article
VL - 17
SP - 1
EP - 10
JO - Bioremediation Journal
JF - Bioremediation Journal
SN - 1088-9868
IS - 1
ER -