Biodegradation of crude oil by constructed bacterial consortia and the constituent single bacteria isolated from Malaysia

Ainon Hamzah, Chia Wei Phan, Nur Faizah Abu Bakar, Kok Kee Wong

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Three bacterial isolates identified as Pseudomonas aeruginosa (UKMP-8T), Rhodococcus sp. M15-2 (UKMP-5T), and Rhodococcus sp. ZH8 (UKMP-7T) based on biochemical, physiological, and morphological characteristics and on 16S rDNA sequences were isolated from groundwater of a crude oil refinery plant. From these three isolates, four bacterial consortia were designed by mixing the single bacterial cultures in the following ratios: (P. aeruginosa:Rhodococcus sp. M15-2, 1:1), (P. aeruginosa:Rhodococcus sp. ZH8, 1:1), (Rhodococcus sp. M15-2: Rhodococcus sp. ZH8, 1:1), and (P. aeruginosa: Rhodococcus sp. ZH8:Rhodococcus sp. M15-2, 1:1:1), respectively. Bacterial isolates and consortia showed differing preferences for nitrogen source (0.01% ammonium chloride, 0.10% yeast extract, or 0.50% peptone) to reach optimum growth. When fortified with the preferred nitrogen sources and grown in minimal salt medium, within 7 days all three single isolates and the four bacterial consortia biodegraded 97.6-99.9% of Tapis Massa oil without any significant differences.

    Original languageEnglish
    Pages (from-to)1-10
    Number of pages10
    JournalBioremediation Journal
    Volume17
    Issue number1
    DOIs
    Publication statusPublished - 2013

    Fingerprint

    crude oil
    biodegradation
    bacterium
    nitrogen
    yeast
    ammonium
    chloride
    salt
    groundwater
    oil
    oil refinery

    Keywords

    • bacterial consortia
    • biodegradation
    • crude oil
    • nitrogen sources
    • Pseudomonas aeruginosa
    • Rhodococcus sp.

    ASJC Scopus subject areas

    • Environmental Science(all)

    Cite this

    Biodegradation of crude oil by constructed bacterial consortia and the constituent single bacteria isolated from Malaysia. / Hamzah, Ainon; Phan, Chia Wei; Abu Bakar, Nur Faizah; Wong, Kok Kee.

    In: Bioremediation Journal, Vol. 17, No. 1, 2013, p. 1-10.

    Research output: Contribution to journalArticle

    Hamzah, Ainon ; Phan, Chia Wei ; Abu Bakar, Nur Faizah ; Wong, Kok Kee. / Biodegradation of crude oil by constructed bacterial consortia and the constituent single bacteria isolated from Malaysia. In: Bioremediation Journal. 2013 ; Vol. 17, No. 1. pp. 1-10.
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