Biocompatible and mucoadhesive bacterial cellulose-g-poly(acrylic acid) hydrogels for oral protein delivery

Naveed Ahmad, Mohd Cairul Iqbal Mohd Amin, Shalela Mohd Mahali, Ismanizan Ismail, Victor Tuan Giam Chuang

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Stimuli-responsive bacterial cellulose-g-poly-(acrylic acid) hydrogels were investigated for their potential use as an oral delivery system for proteins. These hydrogels were synthesized using electron beam irradiation without any cross-linking agents, thereby eliminating any potential toxic effects associated with cross-linkers. Bovine serum albumin (BSA), a model protein drug, was loaded into the hydrogels, and the release profile in simulated gastrointestinal fluids was investigated. Cumulative release of less than 10% in simulated gastric fluid (SGF) demonstrated the potential of these hydrogels to protect BSA from the acidic environment of the stomach. Subsequent conformational stability analyses of released BSA by SDS-PAGE, circular dichroism, and an esterase activity assay indicated that the structural integrity and bioactivity of BSA was maintained and preserved by the hydrogels. Furthermore, an increase in BSA penetration across intestinal mucosa tissue was observed in an ex vivo penetration experiment. Our fabricated hydrogels exhibited excellent cytocompatibility and showed no sign of toxicity, indicating the safety of these hydrogels for in vivo applications.

Original languageEnglish
Pages (from-to)4130-4142
Number of pages13
JournalMolecular Pharmaceutics
Volume11
Issue number11
DOIs
Publication statusPublished - 2014

Fingerprint

carbopol 940
Hydrogels
Cellulose
Bovine Serum Albumin
Proteins
Stomach
Poisons
Esterases
Intestinal Mucosa
Circular Dichroism
Polyacrylamide Gel Electrophoresis

Keywords

  • Bacterial cellulose
  • Biocompatibility
  • Electron beam
  • Hydrogels
  • Oral protein delivery
  • Protein conformational stability

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Molecular Medicine
  • Drug Discovery

Cite this

Biocompatible and mucoadhesive bacterial cellulose-g-poly(acrylic acid) hydrogels for oral protein delivery. / Ahmad, Naveed; Mohd Amin, Mohd Cairul Iqbal; Mahali, Shalela Mohd; Ismail, Ismanizan; Chuang, Victor Tuan Giam.

In: Molecular Pharmaceutics, Vol. 11, No. 11, 2014, p. 4130-4142.

Research output: Contribution to journalArticle

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AU - Ismail, Ismanizan

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