Biochemical events leading to the diversion of carbon into storage lipids in the oleaginous fungi Mucor circinelloides and Mortierella alpina

J. P. Wynn, Aidil Abdul Hamid, Y. Li, C. Ratledge

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

The biochemical events associated with the onset of lipid accumulation in Mucor circinelloides and Mortierella alpina, under conditions of nitrogen-limited growth, have been elucidated; they differ in key aspects from those described in oleaginous yeasts. The NAD+:isocitrate dehydrogenases of Mc. circinelloides and Mort. alpina were not absolutely dependent on AMP for activity. Furthermore, changes in the cellular adenine nucleotide pools and energy charge were different from those reported for oleaginous yeasts. In Mc. circinelloides ATP, ADP and AMP concentrations all decreased by 50% after nitrogen limitation, leading to a constant energy charge at the expense of the size of the total adenylate pool. Pyruvate carboxylase in Mc. circinelloides was cytosolic, having implications for the organization of lipid synthesis in filamentous fungi. As a result of the data obtained, a revised and more concerted mechanism for the initiation of storage lipid accumulation is put forward for filamentous fungi.

Original languageEnglish
Pages (from-to)2857-2864
Number of pages8
JournalMicrobiology
Volume147
Issue number10
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Mortierella
Mucor
Fungi
Carbon
Adenosine Monophosphate
Lipids
Nitrogen
Yeasts
Pyruvate Carboxylase
Isocitrate Dehydrogenase
Adenine Nucleotides
Adenosine Diphosphate
Adenosine Triphosphate
Growth

Keywords

  • AMP deaminase
  • Lipid accumulation
  • NAD: isocitrate dehydrogenase

ASJC Scopus subject areas

  • Microbiology

Cite this

Biochemical events leading to the diversion of carbon into storage lipids in the oleaginous fungi Mucor circinelloides and Mortierella alpina. / Wynn, J. P.; Abdul Hamid, Aidil; Li, Y.; Ratledge, C.

In: Microbiology, Vol. 147, No. 10, 2001, p. 2857-2864.

Research output: Contribution to journalArticle

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