Bioaugmentation of microbial consortia and supplementation of bulking agents in removal of crude oil from soil

A. Hamzah, S. N Md Salleh, S. L. Lee, Sukiman Sarmani

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Biodegradation experiment was carried out to evaluate the effects supplementation of microbial consortium and bulking agents in biodegradation of crude oil in soil. The soil with indigenous microbes was spiked with crude oil at 50,000 ppm and a cocktail of microbial consortium at ratio 1:1:1:1 (v/w) which consist of Pseudomonas sp. UKMP 14-T, Acinetobactersp. UKMP 12-T and two fungi isolates Trichodermasp. (TriUKMP-1M and TriUKMP-2M). Bulking agents (sugarcane baggasse (SB) and empty fruit bunch (EFB) from oil palm) at 15% and 20% (w/w), respectively were added and mixed thoroughly. The pH and moisture content of the soil was maintained at 6.5 and 40% VWC, respectively. The degradation of crude oil from the soil was analyzed using gas chromatography-flame ionization detector (GC-FID) and the growth of bacteria was estimated using spread plate method. The result showed that biodegradation of crude oil by microbial consortium with addition of SB produced 100% Total Petroleum Hydrocarbon (TPH) degradation as compared to 91% with eFB after 30 days incubation. The control plot which contains only indigenous microbes showed 62% degradation at the same period of incubation. The results indicate that the types of the bulking agent may influence the intake of the nutrient source by microbial consortia hence influenced the percentage of the TPH degradation.

Original languageEnglish
Title of host publicationMicrobes in Applied Research: Current Advances and Challenges, Malaga, Spain, 14 - 16 September 2011
PublisherWorld Scientific Publishing Co.
Pages39-43
Number of pages5
ISBN (Electronic)9789814405041
ISBN (Print)9814405035, 9789814405034
DOIs
Publication statusPublished - 1 Jan 2012

Fingerprint

Microbial Consortia
Petroleum
Soil
Soils
Biodegradation
Degradation
Saccharum
Hydrocarbons
Flame Ionization
Palm oil
Fruits
Pseudomonas
Fungi
Gas chromatography
Gas Chromatography
Nutrients
Ionization
Fruit
Bacteria
Oils

Keywords

  • Biodegradation
  • Biostimulation
  • Bulking agent
  • Crude oil
  • Microbial consortium

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Hamzah, A., Salleh, S. N. M., Lee, S. L., & Sarmani, S. (2012). Bioaugmentation of microbial consortia and supplementation of bulking agents in removal of crude oil from soil. In Microbes in Applied Research: Current Advances and Challenges, Malaga, Spain, 14 - 16 September 2011 (pp. 39-43). World Scientific Publishing Co.. https://doi.org/10.1142/9789814405041_0008

Bioaugmentation of microbial consortia and supplementation of bulking agents in removal of crude oil from soil. / Hamzah, A.; Salleh, S. N Md; Lee, S. L.; Sarmani, Sukiman.

Microbes in Applied Research: Current Advances and Challenges, Malaga, Spain, 14 - 16 September 2011. World Scientific Publishing Co., 2012. p. 39-43.

Research output: Chapter in Book/Report/Conference proceedingChapter

Hamzah, A, Salleh, SNM, Lee, SL & Sarmani, S 2012, Bioaugmentation of microbial consortia and supplementation of bulking agents in removal of crude oil from soil. in Microbes in Applied Research: Current Advances and Challenges, Malaga, Spain, 14 - 16 September 2011. World Scientific Publishing Co., pp. 39-43. https://doi.org/10.1142/9789814405041_0008
Hamzah A, Salleh SNM, Lee SL, Sarmani S. Bioaugmentation of microbial consortia and supplementation of bulking agents in removal of crude oil from soil. In Microbes in Applied Research: Current Advances and Challenges, Malaga, Spain, 14 - 16 September 2011. World Scientific Publishing Co. 2012. p. 39-43 https://doi.org/10.1142/9789814405041_0008
Hamzah, A. ; Salleh, S. N Md ; Lee, S. L. ; Sarmani, Sukiman. / Bioaugmentation of microbial consortia and supplementation of bulking agents in removal of crude oil from soil. Microbes in Applied Research: Current Advances and Challenges, Malaga, Spain, 14 - 16 September 2011. World Scientific Publishing Co., 2012. pp. 39-43
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