Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis

A. M. Ali, I. Intan-Safinar, Muhammad Mukram Mohamed Mackeen, S. H. El-Sharkawy, K. Takahata, H. Kanzaki, K. Kawazu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The ethanol extract from the leaves of Juniperus chinensis was found to be cytotoxic towards HeLa cells. Bioassay-guided fractionation of the EtOAc soluble faction directed by the microtitration cytotoxic assay revealed that the cytotoxic compound was deoxypodophyllotoxin. All the tumour cell lines tested (KU8112F-chronic mylogeneous leukemia, TK 10-renal carcinoma, UACC 62- melanoma and CEM-SS - T-lymphoblastic leukemia) were found to be susceptible to deoxypodophyllotoxin, however, the minimum effective concentration (MEC) required to reduce the cell population by 100 percent was different between cell lines.

Original languageEnglish
Pages (from-to)180-183
Number of pages4
JournalNatural Product Sciences
Volume4
Issue number3
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

Juniperus
Bioassay
Biological Assay
Cells
Tumor Cell Line
Precursor Cell Lymphoblastic Leukemia-Lymphoma
HeLa Cells
Melanoma
Leukemia
Ethanol
Fractionation
Carcinoma
Kidney
Cell Line
Tumors
Assays
Population
deoxypodophyllotoxin

Keywords

  • Cytotoxicity
  • Deoxypodophyllotoxin
  • Juniperus chinensis

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

Ali, A. M., Intan-Safinar, I., Mohamed Mackeen, M. M., El-Sharkawy, S. H., Takahata, K., Kanzaki, H., & Kawazu, K. (1998). Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis. Natural Product Sciences, 4(3), 180-183.

Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis. / Ali, A. M.; Intan-Safinar, I.; Mohamed Mackeen, Muhammad Mukram; El-Sharkawy, S. H.; Takahata, K.; Kanzaki, H.; Kawazu, K.

In: Natural Product Sciences, Vol. 4, No. 3, 1998, p. 180-183.

Research output: Contribution to journalArticle

Ali, AM, Intan-Safinar, I, Mohamed Mackeen, MM, El-Sharkawy, SH, Takahata, K, Kanzaki, H & Kawazu, K 1998, 'Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis', Natural Product Sciences, vol. 4, no. 3, pp. 180-183.
Ali, A. M. ; Intan-Safinar, I. ; Mohamed Mackeen, Muhammad Mukram ; El-Sharkawy, S. H. ; Takahata, K. ; Kanzaki, H. ; Kawazu, K. / Bioassay-guided isolation of deoxypodophyllotoxin, the cytotoxic constituent of Juniperus chinensis. In: Natural Product Sciences. 1998 ; Vol. 4, No. 3. pp. 180-183.
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