Bioassay guided isolation of antibacterial compounds from Andrographis paniculata

Abubakar Sule, Qamar Uddin Ahmed, Othman Abd Samah, Muhammad Nor Omar, Norazian M. Hassan, Laina Zarisa M Kamal, Mohd. Ambar Yarmo

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Problem statement: Chronic disease-causing bacteria of medical importance have developed resistance to antibiotics, hence, necessitating distinct and constant need for safe and efficient therapeutic agents. Plants are considered potent candidate for this aim. A way out of reducing antibiotic resistance and adverse effects on host is the employment of antibiotic resistance inhibitors of plant origin. Approach: About 5 kg pulverized Andrographis paniculata whole plant was macerated with MeOH at room temperature to get 305 g freeze dried MeOH extract. The bioautography of MeOH extract using Staphylococcus aureus and Proteus mirabilis as indicator organisms revealed the presence of two potent antibacterial compounds. MeOH extract was further fractionated and purified by silica gel column chromatography which led to the isolation of a diterpene lactone and an entlabdane diterpene glycoside upon crystallization with absolute ethanol. Results: Two antibacterial compounds viz., 3-O-β-D-glucosyl-14-deoxyandrographolide and 14-deoxyandrographolide were successfully isolated and characterized. Their structures were exclusively elucidated through spectroscopic methods (UV, IR, 1H- and 13C NMR). Conclusion: A. paniculata possesses antibacterial activity and could be potential source of a new class of antibiotics that might be useful for infectious disease chemotherapy and control.

Original languageEnglish
Pages (from-to)525-534
Number of pages10
JournalAmerican Journal of Applied Sciences
Volume8
Issue number6
Publication statusPublished - 2011

Fingerprint

Andrographis
Microbial Drug Resistance
Biological Assay
Diterpenes
Proteus mirabilis
Silica Gel
Lactones
Glycosides
Crystallization
Gel Chromatography
Communicable Diseases
Staphylococcus aureus
Chronic Disease
Ethanol
Anti-Bacterial Agents
Bacteria
Drug Therapy
Temperature
14-deoxyandrographolide
Therapeutics

Keywords

  • Andrographis paniculata
  • Antibacterial compounds
  • Bioassay-guided isolation

ASJC Scopus subject areas

  • General

Cite this

Sule, A., Ahmed, Q. U., Samah, O. A., Omar, M. N., Hassan, N. M., Kamal, L. Z. M., & Yarmo, M. A. (2011). Bioassay guided isolation of antibacterial compounds from Andrographis paniculata. American Journal of Applied Sciences, 8(6), 525-534.

Bioassay guided isolation of antibacterial compounds from Andrographis paniculata. / Sule, Abubakar; Ahmed, Qamar Uddin; Samah, Othman Abd; Omar, Muhammad Nor; Hassan, Norazian M.; Kamal, Laina Zarisa M; Yarmo, Mohd. Ambar.

In: American Journal of Applied Sciences, Vol. 8, No. 6, 2011, p. 525-534.

Research output: Contribution to journalArticle

Sule, A, Ahmed, QU, Samah, OA, Omar, MN, Hassan, NM, Kamal, LZM & Yarmo, MA 2011, 'Bioassay guided isolation of antibacterial compounds from Andrographis paniculata', American Journal of Applied Sciences, vol. 8, no. 6, pp. 525-534.
Sule A, Ahmed QU, Samah OA, Omar MN, Hassan NM, Kamal LZM et al. Bioassay guided isolation of antibacterial compounds from Andrographis paniculata. American Journal of Applied Sciences. 2011;8(6):525-534.
Sule, Abubakar ; Ahmed, Qamar Uddin ; Samah, Othman Abd ; Omar, Muhammad Nor ; Hassan, Norazian M. ; Kamal, Laina Zarisa M ; Yarmo, Mohd. Ambar. / Bioassay guided isolation of antibacterial compounds from Andrographis paniculata. In: American Journal of Applied Sciences. 2011 ; Vol. 8, No. 6. pp. 525-534.
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