Bioassay-guided isolation of a potent platelet-activating factor antagonist alkenylresorcinol from Ardisia elliptica

Juriyati Jalil, Ibrahim Jantan, Khozirah Shaari, Iftikhar Ahmad Abdul Rafi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In the course of our search for novel platelet-activating factor (PAF) antagonists from medicinal plants, the methanol extract of the leaves of Ardisia elliptica Thunb. was investigated for its inhibitory effects on PAF receptor binding to rabbit platelets using 3H-PAF as a ligand. The methanol extract showed inhibitory activity of 53.9% and its ethyl acetate, n-butanol, and methanol fractions exhibited 48.6%, 39.0%, and 22.0% inhibition, respectively. Bioassay-guided fractionation of the ethyl acetate fraction led to the isolation of a new alkenylresorcinol, 5-(Z-heptadec-4′-enyl) resorcinol, together with 5-pentadecylresorcinol. The alkenylresorcinol showed a strong inhibition with an IC50 value of 7.1 μM. The structures of the compounds were elucidated by spectroscopic techniques.

Original languageEnglish
Pages (from-to)457-461
Number of pages5
JournalPharmaceutical Biology
Volume42
Issue number6
DOIs
Publication statusPublished - Sep 2004

Fingerprint

Ardisia
platelet-activating factor
Bioassay
Platelet Activating Factor
Biological Assay
Methanol
antagonists
methanol
bioassays
resorcinol
1-Butanol
Plant Extracts
Medicinal Plants
butanol
Fractionation
Platelets
leaf extracts
Inhibitory Concentration 50
medicinal plants
inhibitory concentration 50

Keywords

  • Alkenylresorcinol
  • Ardisia elliptica
  • Bioassay-guided
  • Platelet-activating factor antagonists
  • Platelets

ASJC Scopus subject areas

  • Plant Science
  • Medical Laboratory Technology
  • Clinical Biochemistry
  • Pharmacology

Cite this

Bioassay-guided isolation of a potent platelet-activating factor antagonist alkenylresorcinol from Ardisia elliptica. / Jalil, Juriyati; Jantan, Ibrahim; Shaari, Khozirah; Rafi, Iftikhar Ahmad Abdul.

In: Pharmaceutical Biology, Vol. 42, No. 6, 09.2004, p. 457-461.

Research output: Contribution to journalArticle

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