Bioanalisis berasaskan sistem glutamat dehidrogenase-Diaporase untuk pengesanan ammonium

Translated title of the contribution: Bioanalysis based on glutamate dehydrogenase/Diaphorase system for ammonium determination

Nur Ellina Azmi, Jaafar Abdullah, Musa Ahmad, Yook Heng Lee, Hamidah Sidek, Samsulida Abd Rahman

Research output: Contribution to journalArticle

Abstract

Bioanalysis for ammonium detection based on dual enzymeystem Glutamate dehydrogenase/ Diaphorase (GLDH-Dph) and thiazolyl blue tetrazolium bromide (MTT) reagent has been described. In this work, GLDH will catalyze the conversion of α-ketoglutaric acid to L-glutamate and β-nicotinamide adenine dinucleotide (NADH) will be oxidized to NAD+ in the presence of ammonium. The excess of unreacted NADH was then oxidized to NAD+ by Dph and MTT reagent was reduced to purple color formazan. The intensity of formazan product formed was observed at the wavelength of 563 nm by using spectrophotometer. Bioanalysis showed optimum response for ammonium detection at phosphate buffer of pH 8, GLDH, Dph and MTT concentrations at 13.2 unit/mL, 1.17 unit/mL and 0.2 mM, respectively. Bioanalysis showed linear response towards ammonium in the concentration range of 3 - 50 μM with the detection limit of 1 μM.

Original languageUndefined/Unknown
Pages (from-to)1263-1269
Number of pages7
JournalSains Malaysiana
Volume40
Issue number11
Publication statusPublished - Nov 2011

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Glutamate Dehydrogenase
Ammonium Compounds
NAD
Formazans
Ketoglutaric Acids
Spectrophotometers
Glutamic Acid
Buffers
Phosphates
Color
Wavelength

Keywords

  • Ammonium
  • Bioanalysis
  • Dph
  • GLDH

ASJC Scopus subject areas

  • General

Cite this

Azmi, N. E., Abdullah, J., Ahmad, M., Lee, Y. H., Sidek, H., & Abd Rahman, S. (2011). Bioanalisis berasaskan sistem glutamat dehidrogenase-Diaporase untuk pengesanan ammonium. Sains Malaysiana, 40(11), 1263-1269.

Bioanalisis berasaskan sistem glutamat dehidrogenase-Diaporase untuk pengesanan ammonium. / Azmi, Nur Ellina; Abdullah, Jaafar; Ahmad, Musa; Lee, Yook Heng; Sidek, Hamidah; Abd Rahman, Samsulida.

In: Sains Malaysiana, Vol. 40, No. 11, 11.2011, p. 1263-1269.

Research output: Contribution to journalArticle

Azmi, NE, Abdullah, J, Ahmad, M, Lee, YH, Sidek, H & Abd Rahman, S 2011, 'Bioanalisis berasaskan sistem glutamat dehidrogenase-Diaporase untuk pengesanan ammonium', Sains Malaysiana, vol. 40, no. 11, pp. 1263-1269.
Azmi, Nur Ellina ; Abdullah, Jaafar ; Ahmad, Musa ; Lee, Yook Heng ; Sidek, Hamidah ; Abd Rahman, Samsulida. / Bioanalisis berasaskan sistem glutamat dehidrogenase-Diaporase untuk pengesanan ammonium. In: Sains Malaysiana. 2011 ; Vol. 40, No. 11. pp. 1263-1269.
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