Bioactivity and cell compatibility of β-wollastonite derived from rice husk ash and limestone

Roslinda Shamsudin, Farah 'Atiqah Abdul Azam, Muhammad Azmi Abdul Hamid, Hamisah Ismail

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The aim of this study was to prepare β-wollastonite using a green synthesis method (autoclaving technique) without organic solvents and to study its bioactivity. To prepare β-wollastonite, the precursor ratio of CaO:SiO2 was set at 55:45. This mixture was autoclaved for 8 h and later sintered at 950 °C for 2 h. The chemical composition of the precursors was studied using X-ray fluorescence (XRF), in which rice husk ash consists of 89.5 wt % of SiO2 in a cristobalite phase and calcined limestone contains 97.2 wt % of CaO. The X-ray diffraction (XRD) patterns after sintering showed that only β-wollastonite was detected as the single phase. To study its bioactivity and degradation properties, β-wollastonite samples were immersed in simulated body fluid (SBF) for various periods of time. Throughout the soaking period, the molar ratio of Ca/P obtained was in the range of 1.19 to 2.24, and the phase detected was amorphous calcium phosphate, which was confirmed by scanning electron microscope with energy dispersive X-ray analysis (SEM/EDX) and XRD. Fourier-transform infrared spectroscopy (FTIR) analysis indicated that the peaks of the calcium and phosphate ions increased when an amorphous calcium phosphate layer was formed on the surface of the β-wollastonite sample. A cell viability and proliferation assay test was performed on the rice husk ash, calcined limestone, and β-wollastonite samples by scanning electron microscope. For heavy metal element evaluation, a metal panel that included As, Cd, Pb, and Hg was selected, and both precursor and β-wollastonite fulfilled the requirement of an American Society for Testing and Materials (ASTM F1538-03) standard specification. Apart from that, a degradation test showed that the loss of mass increased incrementally as a function of soaking period. These results showed that the β-wollastonite materials produced from rice husk ash and limestone possessed good bioactivity, offering potential for biomedical applications.

Original languageEnglish
Article number1188
JournalMaterials
Volume10
Issue number10
DOIs
Publication statusPublished - 17 Oct 2017

Fingerprint

Ashes
Calcium Carbonate
Bioactivity
Limestone
Calcium phosphate
Electron microscopes
Scanning
X ray diffraction
Degradation
Energy dispersive X ray analysis
Body fluids
Chemical elements
Organic solvents
Diffraction patterns
Heavy metals
Fourier transform infrared spectroscopy
Calcium
Assays
Phosphates
Sintering

Keywords

  • Autoclaving
  • Bioactive
  • Green synthesize
  • Rice husk ash
  • Wollastonite

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Bioactivity and cell compatibility of β-wollastonite derived from rice husk ash and limestone. / Shamsudin, Roslinda; 'Atiqah Abdul Azam, Farah; Abdul Hamid, Muhammad Azmi; Ismail, Hamisah.

In: Materials, Vol. 10, No. 10, 1188, 17.10.2017.

Research output: Contribution to journalArticle

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