Bioactive endophytic streptomycetes from the Malay Peninsula

Noraziah Mohamad Zin, Nurul I M Sarmin, Norazli Ghadin, Dayang Fredalina Basri, Nik Marzuki Sidik, W. M. Hess, Gary A. Strobel

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Three novel endophytic streptomycetes have been isolated and characterized from plants with ethnobotanical uses on the Malay Peninsula including: Thottea grandiflora (family - Aristolochiaceae), Polyalthia spp. (family - Annonaceae), and Mapania sp. (family - Cyperaceae). Each isolate, as studied by scanning electron microscopy, has small hyphae, and produces typical barrel-shaped spores arising by hyphal fragmentation. Interestingly, although none has any detectable antibacterial killing properties, each has demonstrable killing activity against one or more pathogenic fungi including organisms such as Phytophthora erythroseptica, Pythium ultimum, Sclerotinia sclerotiorum, Mycosphaerella fijiensis and Rhizoctonia solani. Molecular biological studies on the rRNA gene sequence of each isolate revealed that it is distinct from all other genetic accessions of streptomyectes in GenBank, and each bears some genetic similarity to other streptomycetes. The bioactivity of each microbe was extractable in various organic solvents.

Original languageEnglish
Pages (from-to)83-88
Number of pages6
JournalFEMS Microbiology Letters
Volume274
Issue number1
DOIs
Publication statusPublished - Sep 2007

Fingerprint

Aristolochiaceae
Polyalthia
Cyperaceae
Annonaceae
Pythium
Rhizoctonia
Phytophthora
Ascomycota
Hyphae
Malaysia
Nucleic Acid Databases
Spores
rRNA Genes
Electron Scanning Microscopy
Fungi

Keywords

  • Antifungal agents
  • DNA
  • Endophytes
  • rRNA
  • Streptomycetes

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Bioactive endophytic streptomycetes from the Malay Peninsula. / Mohamad Zin, Noraziah; Sarmin, Nurul I M; Ghadin, Norazli; Basri, Dayang Fredalina; Sidik, Nik Marzuki; Hess, W. M.; Strobel, Gary A.

In: FEMS Microbiology Letters, Vol. 274, No. 1, 09.2007, p. 83-88.

Research output: Contribution to journalArticle

Mohamad Zin, Noraziah ; Sarmin, Nurul I M ; Ghadin, Norazli ; Basri, Dayang Fredalina ; Sidik, Nik Marzuki ; Hess, W. M. ; Strobel, Gary A. / Bioactive endophytic streptomycetes from the Malay Peninsula. In: FEMS Microbiology Letters. 2007 ; Vol. 274, No. 1. pp. 83-88.
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