Bio-Hydrogen production using a two-stage fermentation process

W. M. Alalayah, Mohd. Sahaid Kalil, Abdul Amir H. Kadhum, Jamaliah Md Jahim, S. Z S Jaapar, N. M. Alauj

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A two-stage fermentation process consisting of dark and photo-fermentation periods was carried out in a batch reactor. In the first stage, glucose was fermented in the dark stage using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564; CSN1-4) to produce acetate, CO2 and H2. The acetate produced in the first stage is fermented to H2 and CO2 by Rhodobacter sphaeroides NCIMB 8253 for further hydrogen production in the second, illuminated stage. The yield of hydrogen in the first stage was about 3.10 mol H2 (mol glucose)-1 at a glucose concentration of 10 g L-1, pH 6±0.2 and 37°C and the second stage yield was about 1.10-1.25 mol H2 (mol acetic acid)-1 at pH 6.8±0.2 and 32°C, without removal of the Clostridium CSN1-4. The overall yield of hydrogen in the two-stage process, with glucose as the main substrate was higher than single-stage fermentation.

Original languageEnglish
Pages (from-to)1462-1467
Number of pages6
JournalPakistan Journal of Biological Sciences
Volume12
Issue number22
DOIs
Publication statusPublished - 2009

Fingerprint

hydrogen production
fermentation
glucose
hydrogen
Clostridium saccharoperbutylacetonicum
acetates
Rhodobacter sphaeroides
Clostridium
acetic acid

Keywords

  • Dark fermentation
  • Hydrogen production
  • Photo-fermentation
  • Two-stage process

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Bio-Hydrogen production using a two-stage fermentation process. / Alalayah, W. M.; Kalil, Mohd. Sahaid; Kadhum, Abdul Amir H.; Md Jahim, Jamaliah; Jaapar, S. Z S; Alauj, N. M.

In: Pakistan Journal of Biological Sciences, Vol. 12, No. 22, 2009, p. 1462-1467.

Research output: Contribution to journalArticle

Alalayah, W. M. ; Kalil, Mohd. Sahaid ; Kadhum, Abdul Amir H. ; Md Jahim, Jamaliah ; Jaapar, S. Z S ; Alauj, N. M. / Bio-Hydrogen production using a two-stage fermentation process. In: Pakistan Journal of Biological Sciences. 2009 ; Vol. 12, No. 22. pp. 1462-1467.
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AU - Alauj, N. M.

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