Abstract
A two-stage fermentation process consisting of dark and photo-fermentation periods was carried out in a batch reactor. In the first stage, glucose was fermented in the dark stage using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564; CSN1-4) to produce acetate, CO2 and H2. The acetate produced in the first stage is fermented to H2 and CO2 by Rhodobacter sphaeroides NCIMB 8253 for further hydrogen production in the second, illuminated stage. The yield of hydrogen in the first stage was about 3.10 mol H2 (mol glucose)-1 at a glucose concentration of 10 g L-1, pH 6±0.2 and 37°C and the second stage yield was about 1.10-1.25 mol H2 (mol acetic acid)-1 at pH 6.8±0.2 and 32°C, without removal of the Clostridium CSN1-4. The overall yield of hydrogen in the two-stage process, with glucose as the main substrate was higher than single-stage fermentation.
| Original language | English |
|---|---|
| Pages (from-to) | 1462-1467 |
| Number of pages | 6 |
| Journal | Pakistan Journal of Biological Sciences |
| Volume | 12 |
| Issue number | 22 |
| DOIs | |
| Publication status | Published - 2009 |
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Keywords
- Dark fermentation
- Hydrogen production
- Photo-fermentation
- Two-stage process
ASJC Scopus subject areas
- Agronomy and Crop Science
Cite this
Bio-Hydrogen production using a two-stage fermentation process. / Alalayah, W. M.; Kalil, Mohd. Sahaid; Kadhum, Abdul Amir H.; Md Jahim, Jamaliah; Jaapar, S. Z S; Alauj, N. M.
In: Pakistan Journal of Biological Sciences, Vol. 12, No. 22, 2009, p. 1462-1467.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bio-Hydrogen production using a two-stage fermentation process
AU - Alalayah, W. M.
AU - Kalil, Mohd. Sahaid
AU - Kadhum, Abdul Amir H.
AU - Md Jahim, Jamaliah
AU - Jaapar, S. Z S
AU - Alauj, N. M.
PY - 2009
Y1 - 2009
N2 - A two-stage fermentation process consisting of dark and photo-fermentation periods was carried out in a batch reactor. In the first stage, glucose was fermented in the dark stage using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564; CSN1-4) to produce acetate, CO2 and H2. The acetate produced in the first stage is fermented to H2 and CO2 by Rhodobacter sphaeroides NCIMB 8253 for further hydrogen production in the second, illuminated stage. The yield of hydrogen in the first stage was about 3.10 mol H2 (mol glucose)-1 at a glucose concentration of 10 g L-1, pH 6±0.2 and 37°C and the second stage yield was about 1.10-1.25 mol H2 (mol acetic acid)-1 at pH 6.8±0.2 and 32°C, without removal of the Clostridium CSN1-4. The overall yield of hydrogen in the two-stage process, with glucose as the main substrate was higher than single-stage fermentation.
AB - A two-stage fermentation process consisting of dark and photo-fermentation periods was carried out in a batch reactor. In the first stage, glucose was fermented in the dark stage using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564; CSN1-4) to produce acetate, CO2 and H2. The acetate produced in the first stage is fermented to H2 and CO2 by Rhodobacter sphaeroides NCIMB 8253 for further hydrogen production in the second, illuminated stage. The yield of hydrogen in the first stage was about 3.10 mol H2 (mol glucose)-1 at a glucose concentration of 10 g L-1, pH 6±0.2 and 37°C and the second stage yield was about 1.10-1.25 mol H2 (mol acetic acid)-1 at pH 6.8±0.2 and 32°C, without removal of the Clostridium CSN1-4. The overall yield of hydrogen in the two-stage process, with glucose as the main substrate was higher than single-stage fermentation.
KW - Dark fermentation
KW - Hydrogen production
KW - Photo-fermentation
KW - Two-stage process
UR - http://www.scopus.com/inward/record.url?scp=72249093565&partnerID=8YFLogxK
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U2 - 10.3923/pjbs.2009.1462.1467
DO - 10.3923/pjbs.2009.1462.1467
M3 - Article
VL - 12
SP - 1462
EP - 1467
JO - Pakistan Journal of Biological Sciences
JF - Pakistan Journal of Biological Sciences
SN - 1028-8880
IS - 22
ER -