Bio-guided study on Melastoma malabathricum Linn leaves and elucidation of its biological activities

Mourouge Saadi Alwash, Nazlina Ibrahim, Wan Yaacob Wan Ahmad

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The present study aimed to isolate and identify constituents with antibacterial activity from the Methanol extract of Melastoma Malabathricum Leaves (MMML) through bioassay guided fractionation. Furthermore, the study scoped to evaluate the cytotoxic effects of M. malabathricum leaves fractions for possible protective effect of antoxidative constituents present in the fractions. The MMML extract was fractionated by Vacuum Liquid Chromatography (VLC) to afford M. malabathricum leaves fractions (ML1-ML6) of increasing polarities. Antibacterial activity of plant extract, six fractions and two bioactive constituents against Staphylococcus aureus reference strain, Methicillin-Resistant Staphylococcus aureus reference strain (MRSA), eleven clinical MRSA isolates, three clinical Pseudomonas aeruginosa isolates and P. aeruginosa reference strain was evaluated using Minimum Inhibitory Concentration (MIC). The bioactive constituents responsible for antibacterial activity of MMML, fractions were detected by direct TLCbioautography. The data obtained from MIC assay showed that ML5 effectively inhibited growth of all test bacterial pathogens. Direct TLC-bioautography revealed that ML5 had the highest number of antibacterial compounds. Following bioassay-guided fractionation, Kaempferol-3-O-(2", 6"-di-O-p-trans-coumaroyl)-β- glucopyranoside and Kaempferol were isolated from ML5. The structures of bioactive compounds were elucidated using Nuclear Magnetic Resonance (NMR) spectroscopy from 1HNMR and 13CNMR. In addition, the cytotoxic effect of M. malabathricum leaves fractions (ML1-ML6) revealed that ML5 had the highest Cytotoxicity Concentration (CC50) at 0.75 mg Ml-1, an observation traceable to the presence of flavonoids constituents with antioxidant properties. Therefore, the results clearly indicate that MMML, fractions and bioactive constituents have profound antibacterial activity. Furthermore, antoxidative compounds from ML5 fraction exert their effects by enhancing the level of CC50.

Original languageEnglish
Pages (from-to)767-778
Number of pages12
JournalAmerican Journal of Applied Sciences
Volume10
Issue number8
DOIs
Publication statusPublished - 24 Jul 2013

Fingerprint

Microbial Sensitivity Tests
Methicillin-Resistant Staphylococcus aureus
Biological Assay
Pseudomonas aeruginosa
Plant Extracts
Vacuum
Flavonoids
Liquid Chromatography
Methanol
Staphylococcus aureus
Magnetic Resonance Spectroscopy
Antioxidants
Observation
Growth
kaempferol

Keywords

  • Antibacterial
  • Antioxidant
  • Bioactive constituents
  • Cytotoxic activities
  • Melastoma malabathricum leaves

ASJC Scopus subject areas

  • General

Cite this

Bio-guided study on Melastoma malabathricum Linn leaves and elucidation of its biological activities. / Alwash, Mourouge Saadi; Ibrahim, Nazlina; Wan Ahmad, Wan Yaacob.

In: American Journal of Applied Sciences, Vol. 10, No. 8, 24.07.2013, p. 767-778.

Research output: Contribution to journalArticle

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abstract = "The present study aimed to isolate and identify constituents with antibacterial activity from the Methanol extract of Melastoma Malabathricum Leaves (MMML) through bioassay guided fractionation. Furthermore, the study scoped to evaluate the cytotoxic effects of M. malabathricum leaves fractions for possible protective effect of antoxidative constituents present in the fractions. The MMML extract was fractionated by Vacuum Liquid Chromatography (VLC) to afford M. malabathricum leaves fractions (ML1-ML6) of increasing polarities. Antibacterial activity of plant extract, six fractions and two bioactive constituents against Staphylococcus aureus reference strain, Methicillin-Resistant Staphylococcus aureus reference strain (MRSA), eleven clinical MRSA isolates, three clinical Pseudomonas aeruginosa isolates and P. aeruginosa reference strain was evaluated using Minimum Inhibitory Concentration (MIC). The bioactive constituents responsible for antibacterial activity of MMML, fractions were detected by direct TLCbioautography. The data obtained from MIC assay showed that ML5 effectively inhibited growth of all test bacterial pathogens. Direct TLC-bioautography revealed that ML5 had the highest number of antibacterial compounds. Following bioassay-guided fractionation, Kaempferol-3-O-(2{"}, 6{"}-di-O-p-trans-coumaroyl)-β- glucopyranoside and Kaempferol were isolated from ML5. The structures of bioactive compounds were elucidated using Nuclear Magnetic Resonance (NMR) spectroscopy from 1HNMR and 13CNMR. In addition, the cytotoxic effect of M. malabathricum leaves fractions (ML1-ML6) revealed that ML5 had the highest Cytotoxicity Concentration (CC50) at 0.75 mg Ml-1, an observation traceable to the presence of flavonoids constituents with antioxidant properties. Therefore, the results clearly indicate that MMML, fractions and bioactive constituents have profound antibacterial activity. Furthermore, antoxidative compounds from ML5 fraction exert their effects by enhancing the level of CC50.",
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