Bio-active constituents of rotenoids resin extracted from Derris elliptica roots: Comparison between local plant extract and SAPHYR (France) cube resin

Saiful Irwan Zubairi, Mohamad Roji Sarmidi, Ramlan Abdul Aziz

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

It is well known now that some plant species represent an efficient factory of chemicals, which are manufactured and used as bio-weapons against pest attacks. Extensive work has been done since the last few decades on these potentially useful compounds. During the last few decades a growing interest was directed towards a safer agricultural production such as free residual toxicity hazards to human beings and to the environment. For instance, plant-based extracts biocides possess a greater advantage compared with the chemical ones. Their efficacies are also acceptable. In this work, the aim is to standardize and determine the bio-active constituents extracted from local plant of Derris species (Derris elliptica) by using an internal standard method of reversed-phase high performance liquid chromatography (RP-HPLC) system. The raw plants were collected from Kota Johor Lama, Malaysia and sorted to collect the roots and stems. Rotenoids resin from the roots and stems were extracted by using a normal soaking extraction (NSE) method at 30 ± 2 °C. A solvent of acetone with an optimum solvent-to-solid ratio of 10 ml/g was utilized in the extraction process. The extraction was carried out for 24 hrs and the extract was filtered to remove any fine debris prior to the RP-HPLC analysis. The commercially available rotenoids resin cube of SAPHYR (France) was analyzed to compare and verify the bio-active constituents available with the extract of local plant species. The RP-HPLC analysis results showed the elicited distinguishable patterns of the bio-active constituents between the extract of Derris elliptica and commercial grade of rotenoids cube resin. This extract has a great potential to be used as insecticidal products. However, the rotenone content is still low as compared to the commercial grade rotenoids resin. For that reason, a plant tissue culture is needed to produce a hybrid species between Derris elliptica and Amazonia species so that high yield of rotenone could be attained.

Original languageEnglish
Pages (from-to)904-909
Number of pages6
JournalAdvances in Environmental Biology
Volume8
Issue number4
Publication statusPublished - 2014

Fingerprint

Derris elliptica
Derris
rotenoids
plant extract
Plant Extracts
plant extracts
resins
France
resin
Reverse-Phase Chromatography
liquid chromatography
reversed-phase high performance liquid chromatography
Rotenone
High Pressure Liquid Chromatography
extracts
rotenone
stem
Lama
Weapons
Disinfectants

Keywords

  • Cube resin
  • Derris elliptica
  • HPLC
  • Internal standard
  • Rotenoids
  • Rotenone

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Environmental Science(all)

Cite this

Bio-active constituents of rotenoids resin extracted from Derris elliptica roots : Comparison between local plant extract and SAPHYR (France) cube resin. / Zubairi, Saiful Irwan; Sarmidi, Mohamad Roji; Aziz, Ramlan Abdul.

In: Advances in Environmental Biology, Vol. 8, No. 4, 2014, p. 904-909.

Research output: Contribution to journalArticle

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