Binding at and transactivation of the COX-2 promoter by nuclear tyrosine kinase receptor ErbB-2

Shao Chun Wang, Huang Chun Lien, Weiya Xia, I. Fen Chen, Hui Wen Lo, Zhiqin Wang, Mohamed Ali-Seyed, Dung Fang Lee, Geoffrey Bartholomeusz, Fu Ou-Yang, Dipak K. Giri, Mien Chie Hung

Research output: Contribution to journalArticle

218 Citations (Scopus)

Abstract

Pathological expression of human ErbB-2 protein, also known as HER-2, is common in many types of cancer. ErbB-2 is a member of the EGF receptor tyrosine kinase family and has been rigorously studied as a signaling molecule on the cell membrane. Here, we report that ErbB-2 is also expressed in the nucleus in cultured cells as well as primary tumor tissues. Nuclear ErbB-2 was found to associate with multiple genomic targets in vivo, including the cyclooxygenase enzyme COX-2 gene promoter. ErbB-2 forms a complex at a specific nucleotide sequence of the COX-2 promoter and is able to stimulate its transcription. This study demonstrates the presence of ErbB-2 in the nucleus and identifies the function of ErbB-2 as a transcriptional regulator.

Original languageEnglish
Pages (from-to)251-261
Number of pages11
JournalCancer Cell
Volume6
Issue number3
DOIs
Publication statusPublished - Sep 2004
Externally publishedYes

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Receptor Protein-Tyrosine Kinases
Transcriptional Activation
Prostaglandin-Endoperoxide Synthases
Epidermal Growth Factor Receptor
Protein-Tyrosine Kinases
Cultured Cells
Neoplasms
Cell Membrane
Enzymes
Genes
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Oncology

Cite this

Wang, S. C., Lien, H. C., Xia, W., Chen, I. F., Lo, H. W., Wang, Z., ... Hung, M. C. (2004). Binding at and transactivation of the COX-2 promoter by nuclear tyrosine kinase receptor ErbB-2. Cancer Cell, 6(3), 251-261. https://doi.org/10.1016/j.ccr.2004.07.012

Binding at and transactivation of the COX-2 promoter by nuclear tyrosine kinase receptor ErbB-2. / Wang, Shao Chun; Lien, Huang Chun; Xia, Weiya; Chen, I. Fen; Lo, Hui Wen; Wang, Zhiqin; Ali-Seyed, Mohamed; Lee, Dung Fang; Bartholomeusz, Geoffrey; Ou-Yang, Fu; Giri, Dipak K.; Hung, Mien Chie.

In: Cancer Cell, Vol. 6, No. 3, 09.2004, p. 251-261.

Research output: Contribution to journalArticle

Wang, SC, Lien, HC, Xia, W, Chen, IF, Lo, HW, Wang, Z, Ali-Seyed, M, Lee, DF, Bartholomeusz, G, Ou-Yang, F, Giri, DK & Hung, MC 2004, 'Binding at and transactivation of the COX-2 promoter by nuclear tyrosine kinase receptor ErbB-2', Cancer Cell, vol. 6, no. 3, pp. 251-261. https://doi.org/10.1016/j.ccr.2004.07.012
Wang, Shao Chun ; Lien, Huang Chun ; Xia, Weiya ; Chen, I. Fen ; Lo, Hui Wen ; Wang, Zhiqin ; Ali-Seyed, Mohamed ; Lee, Dung Fang ; Bartholomeusz, Geoffrey ; Ou-Yang, Fu ; Giri, Dipak K. ; Hung, Mien Chie. / Binding at and transactivation of the COX-2 promoter by nuclear tyrosine kinase receptor ErbB-2. In: Cancer Cell. 2004 ; Vol. 6, No. 3. pp. 251-261.
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