Beneficial effects of vitamin e isomer supplementation on static and dynamic bone histomorphometry parameters in normal male rats

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Abstract

Bone is a specialized connective tissue that functions as the load-bearing structure of the body. Free radicals may affect bone remodeling by regulating osteoclast activity in either the physiological or pathological condition. Vitamin E, a lipid-soluble antioxidant, has been demonstrated to offer protection against osteoporosis and to improve the bone material and structure of animal models. The aim of this study was to observe and compare the effects of alpha-tocopherol (α-tocopherol), delta-tocotrienol (δ- tocotrienol), and gamma-tocotrienol (γ-tocotrienol) on the static and dynamic bone histomorphometric parameters in normal male rats. Thirty-two normal Sprague-Dawley male rats aged 3 months and weighing 200-250 g were randomly divided into four groups. The control group was supplemented with oral gavages of olive oil (vehicle), whereas the α-tocopherol, δ-tocotrienol, and γ-tocotrienol groups were given oral gavages of 60 mg/kg α-tocopherol, δ-tocotrienol, and γ-tocotrienol, respectively. The rats were injected twice with calcein to fluorochrome-label the bones. After 4 months of treatment, the rats were killed, and the left femurs were dissected out and prepared for bone histomorphometry. Both the static and dynamic parameters of the vitamin E-treated groups were better than those of the normal control group. Among the vitamin E-treated groups, the tocotrienol groups showed better histomorphometry results compared to the α-tocopherol group, with the γ-tocotrienol group demonstrating the best effects on both sets of parameters. We concluded that vitamin E can promote bone formation in normal rats, with γ-tocotrienol being the most potent form of vitamin E.

Original languageEnglish
Pages (from-to)503-509
Number of pages7
JournalJournal of Bone and Mineral Metabolism
Volume28
Issue number5
DOIs
Publication statusPublished - Sep 2010

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Tocotrienols
Vitamins
Vitamin E
Bone and Bones
Tocopherols
Control Groups
Bone Remodeling
Weight-Bearing
alpha-Tocopherol
Osteoclasts
Fluorescent Dyes
Osteogenesis
Connective Tissue
Femur
Osteoporosis
Free Radicals
Sprague Dawley Rats
Animal Models
Antioxidants
Lipids

Keywords

  • Bone
  • Bone histomorphometry
  • Tocopherol
  • Tocotrienol
  • Vitamin E

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine

Cite this

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title = "Beneficial effects of vitamin e isomer supplementation on static and dynamic bone histomorphometry parameters in normal male rats",
abstract = "Bone is a specialized connective tissue that functions as the load-bearing structure of the body. Free radicals may affect bone remodeling by regulating osteoclast activity in either the physiological or pathological condition. Vitamin E, a lipid-soluble antioxidant, has been demonstrated to offer protection against osteoporosis and to improve the bone material and structure of animal models. The aim of this study was to observe and compare the effects of alpha-tocopherol (α-tocopherol), delta-tocotrienol (δ- tocotrienol), and gamma-tocotrienol (γ-tocotrienol) on the static and dynamic bone histomorphometric parameters in normal male rats. Thirty-two normal Sprague-Dawley male rats aged 3 months and weighing 200-250 g were randomly divided into four groups. The control group was supplemented with oral gavages of olive oil (vehicle), whereas the α-tocopherol, δ-tocotrienol, and γ-tocotrienol groups were given oral gavages of 60 mg/kg α-tocopherol, δ-tocotrienol, and γ-tocotrienol, respectively. The rats were injected twice with calcein to fluorochrome-label the bones. After 4 months of treatment, the rats were killed, and the left femurs were dissected out and prepared for bone histomorphometry. Both the static and dynamic parameters of the vitamin E-treated groups were better than those of the normal control group. Among the vitamin E-treated groups, the tocotrienol groups showed better histomorphometry results compared to the α-tocopherol group, with the γ-tocotrienol group demonstrating the best effects on both sets of parameters. We concluded that vitamin E can promote bone formation in normal rats, with γ-tocotrienol being the most potent form of vitamin E.",
keywords = "Bone, Bone histomorphometry, Tocopherol, Tocotrienol, Vitamin E",
author = "Mehat, {Muhammad Zulfadli} and Shuid, {Ahmad Nazrun} and Norazlina Mohamed and Norliza Muhammad and Soelaiman, {Ima Nirwana}",
year = "2010",
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language = "English",
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T1 - Beneficial effects of vitamin e isomer supplementation on static and dynamic bone histomorphometry parameters in normal male rats

AU - Mehat, Muhammad Zulfadli

AU - Shuid, Ahmad Nazrun

AU - Mohamed, Norazlina

AU - Muhammad, Norliza

AU - Soelaiman, Ima Nirwana

PY - 2010/9

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N2 - Bone is a specialized connective tissue that functions as the load-bearing structure of the body. Free radicals may affect bone remodeling by regulating osteoclast activity in either the physiological or pathological condition. Vitamin E, a lipid-soluble antioxidant, has been demonstrated to offer protection against osteoporosis and to improve the bone material and structure of animal models. The aim of this study was to observe and compare the effects of alpha-tocopherol (α-tocopherol), delta-tocotrienol (δ- tocotrienol), and gamma-tocotrienol (γ-tocotrienol) on the static and dynamic bone histomorphometric parameters in normal male rats. Thirty-two normal Sprague-Dawley male rats aged 3 months and weighing 200-250 g were randomly divided into four groups. The control group was supplemented with oral gavages of olive oil (vehicle), whereas the α-tocopherol, δ-tocotrienol, and γ-tocotrienol groups were given oral gavages of 60 mg/kg α-tocopherol, δ-tocotrienol, and γ-tocotrienol, respectively. The rats were injected twice with calcein to fluorochrome-label the bones. After 4 months of treatment, the rats were killed, and the left femurs were dissected out and prepared for bone histomorphometry. Both the static and dynamic parameters of the vitamin E-treated groups were better than those of the normal control group. Among the vitamin E-treated groups, the tocotrienol groups showed better histomorphometry results compared to the α-tocopherol group, with the γ-tocotrienol group demonstrating the best effects on both sets of parameters. We concluded that vitamin E can promote bone formation in normal rats, with γ-tocotrienol being the most potent form of vitamin E.

AB - Bone is a specialized connective tissue that functions as the load-bearing structure of the body. Free radicals may affect bone remodeling by regulating osteoclast activity in either the physiological or pathological condition. Vitamin E, a lipid-soluble antioxidant, has been demonstrated to offer protection against osteoporosis and to improve the bone material and structure of animal models. The aim of this study was to observe and compare the effects of alpha-tocopherol (α-tocopherol), delta-tocotrienol (δ- tocotrienol), and gamma-tocotrienol (γ-tocotrienol) on the static and dynamic bone histomorphometric parameters in normal male rats. Thirty-two normal Sprague-Dawley male rats aged 3 months and weighing 200-250 g were randomly divided into four groups. The control group was supplemented with oral gavages of olive oil (vehicle), whereas the α-tocopherol, δ-tocotrienol, and γ-tocotrienol groups were given oral gavages of 60 mg/kg α-tocopherol, δ-tocotrienol, and γ-tocotrienol, respectively. The rats were injected twice with calcein to fluorochrome-label the bones. After 4 months of treatment, the rats were killed, and the left femurs were dissected out and prepared for bone histomorphometry. Both the static and dynamic parameters of the vitamin E-treated groups were better than those of the normal control group. Among the vitamin E-treated groups, the tocotrienol groups showed better histomorphometry results compared to the α-tocopherol group, with the γ-tocotrienol group demonstrating the best effects on both sets of parameters. We concluded that vitamin E can promote bone formation in normal rats, with γ-tocotrienol being the most potent form of vitamin E.

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