Batch and continuous thermophilic hydrogen fermentation of sucrose using anaerobic sludge from palm oil mill effluent via immobilisation technique

Nabilah Aminah Lutpi, Jamaliah Md Jahim, Tabassum Mumtaz, Shuhaida Harun, Peer Mohamed Abdul

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

This study aimed to investigate the feasibility to use immobilised cells on granular activated carbon (GAC) operated in fifteen cycles of repeated batch mode for enhancement of biohydrogen production under thermophilic conditions. The effects of the initial pH (5.0-6.5), sucrose concentration (13-43mM) and repeated batch cultivation on biohydrogen production from sucrose were investigated using anaerobic sludge from a palm oil mill effluent treatment plant. The cumulative hydrogen production results were fitted into a modified Gompertz equation in order to find the optimum operational conditions. The optimal hydrogen yield (2.8molH2/molhexose) was obtained at an initial pH of 5.5 and sucrose concentration of 13mM after fifteen cycles of repeated batch. Enriched granular activated carbon (GAC)-immobilised cells from the repeated batch cultivation were used as the immobilised seed culture for anaerobic fermentation of sucrose into hydrogen in continuous operation using a fluidised-bed column reactor (FBCR). The maximum hydrogen production rate (HPR) was found to be 2.7mmolH2/L/h and the hydrogen yield peaked at 2.8molH2/molhexose_consumed after a hydraulic retention time of 12h. The main soluble metabolites were identified as acetic acid, butyric acid and ethanol. The hydrogen content ranged from 48 to 50% of the total biogas.

Original languageEnglish
JournalProcess Biochemistry
DOIs
Publication statusAccepted/In press - 12 Mar 2015

Fingerprint

Palm oil
Sugar (sucrose)
Sewage sludge
Sewage
Immobilization
Fermentation
Sucrose
Effluents
Hydrogen
Hydrogen production
Activated carbon
Immobilized Cells
Cells
Effluent treatment
Butyric acid
Butyric Acid
Biofuels
Biogas
Carbon
Metabolites

Keywords

  • Activated carbon
  • Biohydrogen
  • Immobilisation
  • Repeated batch
  • Thermophilic

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

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abstract = "This study aimed to investigate the feasibility to use immobilised cells on granular activated carbon (GAC) operated in fifteen cycles of repeated batch mode for enhancement of biohydrogen production under thermophilic conditions. The effects of the initial pH (5.0-6.5), sucrose concentration (13-43mM) and repeated batch cultivation on biohydrogen production from sucrose were investigated using anaerobic sludge from a palm oil mill effluent treatment plant. The cumulative hydrogen production results were fitted into a modified Gompertz equation in order to find the optimum operational conditions. The optimal hydrogen yield (2.8molH2/molhexose) was obtained at an initial pH of 5.5 and sucrose concentration of 13mM after fifteen cycles of repeated batch. Enriched granular activated carbon (GAC)-immobilised cells from the repeated batch cultivation were used as the immobilised seed culture for anaerobic fermentation of sucrose into hydrogen in continuous operation using a fluidised-bed column reactor (FBCR). The maximum hydrogen production rate (HPR) was found to be 2.7mmolH2/L/h and the hydrogen yield peaked at 2.8molH2/molhexose_consumed after a hydraulic retention time of 12h. The main soluble metabolites were identified as acetic acid, butyric acid and ethanol. The hydrogen content ranged from 48 to 50{\%} of the total biogas.",
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T1 - Batch and continuous thermophilic hydrogen fermentation of sucrose using anaerobic sludge from palm oil mill effluent via immobilisation technique

AU - Lutpi, Nabilah Aminah

AU - Md Jahim, Jamaliah

AU - Mumtaz, Tabassum

AU - Harun, Shuhaida

AU - Abdul, Peer Mohamed

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N2 - This study aimed to investigate the feasibility to use immobilised cells on granular activated carbon (GAC) operated in fifteen cycles of repeated batch mode for enhancement of biohydrogen production under thermophilic conditions. The effects of the initial pH (5.0-6.5), sucrose concentration (13-43mM) and repeated batch cultivation on biohydrogen production from sucrose were investigated using anaerobic sludge from a palm oil mill effluent treatment plant. The cumulative hydrogen production results were fitted into a modified Gompertz equation in order to find the optimum operational conditions. The optimal hydrogen yield (2.8molH2/molhexose) was obtained at an initial pH of 5.5 and sucrose concentration of 13mM after fifteen cycles of repeated batch. Enriched granular activated carbon (GAC)-immobilised cells from the repeated batch cultivation were used as the immobilised seed culture for anaerobic fermentation of sucrose into hydrogen in continuous operation using a fluidised-bed column reactor (FBCR). The maximum hydrogen production rate (HPR) was found to be 2.7mmolH2/L/h and the hydrogen yield peaked at 2.8molH2/molhexose_consumed after a hydraulic retention time of 12h. The main soluble metabolites were identified as acetic acid, butyric acid and ethanol. The hydrogen content ranged from 48 to 50% of the total biogas.

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