Barrientosiimonas humi gen. nov., sp. nov., an actinobacterium of the family Dermacoccaceae

Learn Han Lee, Yoke Kqueen Cheah, Shiran Mohd Sidik, Qing Yi Xie, Yi Li Tang, Hai Peng Lin, Nurul Syakima Ab Mutalib, Kui Hong

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Three novel actinobacteria, strains 39T, 40 and 41, were isolated from soil collected from Barrientos Island in the Antarctic. The taxonomic status of these strains was determined using a polyphasic approach. Comparison of 16S rRNA gene sequences revealed that strain 39T represented a novel lineage within the family Dermacoccaceae and was most closely related to members of the genera Demetria (96.9% 16S rRNA gene sequence similarity), Branchiibius (95.7%), Dermacoccus (94.4-95.3%), Calidifontibacter (94.6%), Luteipulveratus (94.3%), Yimella (94.2%) and Kytococcus (93.1%). Cells were irregular cocci and short rods. The peptidoglycan type was A4a with an L-Lys-L-Ser-D-Asp interpeptide bridge. The cell-wall sugars were galactose and glucose. The major menaquinone was MK-8(H4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphoglycolipid, two glycolipids and one unknown phospholipid. The acyl type of the cell-wall polysaccharide was N-acetyl. The major cellular fatty acids were anteiso-C17: 0 (41.97%), anteiso-C17: 1ω9c (32.16%) and iso-C16: 0 (7.68%). The DNA G+C content of strain 39T was 68.4 mol%. On the basis of phylogenetic and phenotypic differences from other genera of the family Dermacoccaceae, a novel genus and species, Barrientosiimonas humi gen. nov., sp. nov., is proposed; the type strain of the type species is 39T (=CGMCC 4.6864T =DSM 24617T).

Original languageEnglish
Pages (from-to)241-248
Number of pages8
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume63
Issue number1
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Dermacoccaceae
Actinobacteria
rRNA Genes
Cell Wall
Vitamin K 2
Phosphatidylglycerols
Cardiolipins
Peptidoglycan
gene
new species
Glycolipids
Base Composition
Phosphatidylinositols
phospholipid
Galactose
polysaccharide
Islands
Polysaccharides
Phospholipids
sugar

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Barrientosiimonas humi gen. nov., sp. nov., an actinobacterium of the family Dermacoccaceae. / Lee, Learn Han; Cheah, Yoke Kqueen; Sidik, Shiran Mohd; Xie, Qing Yi; Tang, Yi Li; Lin, Hai Peng; Ab Mutalib, Nurul Syakima; Hong, Kui.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 63, No. 1, 2013, p. 241-248.

Research output: Contribution to journalArticle

Lee, Learn Han ; Cheah, Yoke Kqueen ; Sidik, Shiran Mohd ; Xie, Qing Yi ; Tang, Yi Li ; Lin, Hai Peng ; Ab Mutalib, Nurul Syakima ; Hong, Kui. / Barrientosiimonas humi gen. nov., sp. nov., an actinobacterium of the family Dermacoccaceae. In: International Journal of Systematic and Evolutionary Microbiology. 2013 ; Vol. 63, No. 1. pp. 241-248.
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abstract = "Three novel actinobacteria, strains 39T, 40 and 41, were isolated from soil collected from Barrientos Island in the Antarctic. The taxonomic status of these strains was determined using a polyphasic approach. Comparison of 16S rRNA gene sequences revealed that strain 39T represented a novel lineage within the family Dermacoccaceae and was most closely related to members of the genera Demetria (96.9{\%} 16S rRNA gene sequence similarity), Branchiibius (95.7{\%}), Dermacoccus (94.4-95.3{\%}), Calidifontibacter (94.6{\%}), Luteipulveratus (94.3{\%}), Yimella (94.2{\%}) and Kytococcus (93.1{\%}). Cells were irregular cocci and short rods. The peptidoglycan type was A4a with an L-Lys-L-Ser-D-Asp interpeptide bridge. The cell-wall sugars were galactose and glucose. The major menaquinone was MK-8(H4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphoglycolipid, two glycolipids and one unknown phospholipid. The acyl type of the cell-wall polysaccharide was N-acetyl. The major cellular fatty acids were anteiso-C17: 0 (41.97{\%}), anteiso-C17: 1ω9c (32.16{\%}) and iso-C16: 0 (7.68{\%}). The DNA G+C content of strain 39T was 68.4 mol{\%}. On the basis of phylogenetic and phenotypic differences from other genera of the family Dermacoccaceae, a novel genus and species, Barrientosiimonas humi gen. nov., sp. nov., is proposed; the type strain of the type species is 39T (=CGMCC 4.6864T =DSM 24617T).",
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AU - Lee, Learn Han

AU - Cheah, Yoke Kqueen

AU - Sidik, Shiran Mohd

AU - Xie, Qing Yi

AU - Tang, Yi Li

AU - Lin, Hai Peng

AU - Ab Mutalib, Nurul Syakima

AU - Hong, Kui

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AB - Three novel actinobacteria, strains 39T, 40 and 41, were isolated from soil collected from Barrientos Island in the Antarctic. The taxonomic status of these strains was determined using a polyphasic approach. Comparison of 16S rRNA gene sequences revealed that strain 39T represented a novel lineage within the family Dermacoccaceae and was most closely related to members of the genera Demetria (96.9% 16S rRNA gene sequence similarity), Branchiibius (95.7%), Dermacoccus (94.4-95.3%), Calidifontibacter (94.6%), Luteipulveratus (94.3%), Yimella (94.2%) and Kytococcus (93.1%). Cells were irregular cocci and short rods. The peptidoglycan type was A4a with an L-Lys-L-Ser-D-Asp interpeptide bridge. The cell-wall sugars were galactose and glucose. The major menaquinone was MK-8(H4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphoglycolipid, two glycolipids and one unknown phospholipid. The acyl type of the cell-wall polysaccharide was N-acetyl. The major cellular fatty acids were anteiso-C17: 0 (41.97%), anteiso-C17: 1ω9c (32.16%) and iso-C16: 0 (7.68%). The DNA G+C content of strain 39T was 68.4 mol%. On the basis of phylogenetic and phenotypic differences from other genera of the family Dermacoccaceae, a novel genus and species, Barrientosiimonas humi gen. nov., sp. nov., is proposed; the type strain of the type species is 39T (=CGMCC 4.6864T =DSM 24617T).

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