Bacteriocin production by a marine strain of Bacills sp. Sh10

Isolation, screening and optimization of culture condition

Fatemeh Shayesteh, Asmat Ahmad, Gires Usup

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Antibacterial peptides have emerged as an alternative therapeutic to protect food and other products from deterioration and spoilage. In this study, bacteria were isolated from marine clams and screened for bacteriocin production using cross-streaking and spot-on-lawn methods. One of the isolates, identified as Bacillus species Sh10, produced proteinaceous bacteriocin with a broad spectrum of activity against human pathogens and food-spoilage bacteria. The effect of different media compositions and culture conditions namely, the type and concentration of carbon and nitrogen source, NaCl concentration, temperature, pH, aeration and incubation time on the production of bacteriocin by Bacillus sp. Sh10 was investigated. Optimum bacteriocin production was recorded when 2% tryptone and 1% glucose were added to a basal medium as nitrogen and carbon sources, respectively. The bacteria exhibited maximum bacteriocin production at 2% NaCl, pH 8, 30°C and 200 rev min-1 aeration. Bacteriocin was produced during the stationary phase, indicating it is synthesized as a secondary metabolite. Bacillus sp. Sh10 is able to produce bacteriocin at a wide pH and temperature range making it a good candidate for the production of bacteriocin on an industrial scale level.

Original languageEnglish
Pages (from-to)273-281
Number of pages9
JournalBiotechnology
Volume13
Issue number6
DOIs
Publication statusPublished - 2014

Fingerprint

Bacteriocins
Screening
Bacilli
Bacillus
Spoilage
Bacteria
Nitrogen
Carbon
Food
Temperature
Bivalvia
Pathogens
Metabolites
Human Activities
Culture Media
Deterioration
Glucose
Peptides
Chemical analysis

Keywords

  • Bacillus sp. Sh10
  • Bacteriocin
  • Marine clams
  • Optimization

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Bacteriocin production by a marine strain of Bacills sp. Sh10 : Isolation, screening and optimization of culture condition. / Shayesteh, Fatemeh; Ahmad, Asmat; Usup, Gires.

In: Biotechnology, Vol. 13, No. 6, 2014, p. 273-281.

Research output: Contribution to journalArticle

Shayesteh, Fatemeh ; Ahmad, Asmat ; Usup, Gires. / Bacteriocin production by a marine strain of Bacills sp. Sh10 : Isolation, screening and optimization of culture condition. In: Biotechnology. 2014 ; Vol. 13, No. 6. pp. 273-281.
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