Bactericidal effect of pterostilbene alone and in combination with gentamicin against human pathogenic bacteria

Wee Xian Lee, Dayang Fredalina Basri, Ahmad Rohi Ghazali, Philippe Jeandet

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The antibacterial activity of pterostilbene in combination with gentamicin against six strains of Gram-positive and Gram-negative bacteria were investigated. The minimum inhibitory concentration and minimum bactericidal concentration of pterostilbene were determined using microdilution technique whereas the synergistic antibacterial activities of pterostilbene in combination with gentamicin were assessed using checkerboard assay and time-kill kinetic study. Results of the present study showed that the combination effects of pterostilbene with gentamicin were synergistic (FIC index < 0.5) against three susceptible bacteria strains: Staphylococcus aureus ATCC 25923, Escherichia coli O157 and Pseudomonas aeruginosa 15442. However, the time-kill study showed that the interaction was indifference which did not significantly differ from the gentamicin treatment. Furthermore, time-kill study showed that the growth of the tested bacteria was completely attenuated with 2 to 8 h treatment with 0.5 × MIC of pterostilbene and gentamicin. The identified combinations could be of effective therapeutic value against bacterial infections. These findings have potential implications in delaying the development of bacterial resistance as the antibacterial effect was achieved with the lower concentrations of antibacterial agents.

Original languageEnglish
Article number463
JournalMolecules
Volume22
Issue number3
DOIs
Publication statusPublished - 1 Mar 2017

Fingerprint

Gentamicins
bacteria
Bacteria
Time and motion study
pseudomonas
staphylococcus
infectious diseases
Escherichia
Escherichia coli O157
Microbial Sensitivity Tests
low concentrations
Gram-Negative Bacteria
Bacterial Infections
Pseudomonas aeruginosa
Escherichia coli
Staphylococcus aureus
Assays
pterostilbene
Anti-Bacterial Agents
kinetics

ASJC Scopus subject areas

  • Medicine(all)
  • Organic Chemistry

Cite this

Bactericidal effect of pterostilbene alone and in combination with gentamicin against human pathogenic bacteria. / Lee, Wee Xian; Basri, Dayang Fredalina; Ghazali, Ahmad Rohi; Jeandet, Philippe.

In: Molecules, Vol. 22, No. 3, 463, 01.03.2017.

Research output: Contribution to journalArticle

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