Bacterial community structure, compartmentalization and activity in a microbial fuel cell

G. T. Kim, G. Webster, J. W T Wimpenny, B. H. Kim, H. J. Kim, A. J. Weightman

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

Aims: To characterize bacterial populations and their activities within a microbial fuel cell (MFC), using cultivation-independent and cultivation approaches. Methods and Results: Electron microscopic observations showed that the fuel cell electrode had a microbial biofilm attached to its surface with loosely associated microbial clumps. Bacterial 16S rRNA gene libraries were constructed and analysed from each of four compartments within the fuel cell: the planktonic community; the membrane biofilm; bacterial clumps (BC) and the anode biofilm. Results showed that the bacterial community structure varied significantly between these compartments. It was observed that Gammaproteobacteria phylotypes were present at higher numbers within libraries from the BC and electrode biofilm compared with other parts of the fuel cell. Community structure of the MFC determined by analyses of bacterial 16S rRNA gene libraries and anaerobic cultivation showed excellent agreement with community profiles from denaturing gradient gel electrophoresis (DGGE) analysis. Conclusions: Members of the family Enterobacteriaceae, such as Klebsiella sp. and Enterobacter sp. and other Gammaproteobacteria with Fe(III)-reducing and electrochemical activity had a significant potential for energy generation in this system. Significance and Impact of the Study: This study has shown that electrochemically active bacteria can be enriched using an electrochemical fuel cell.

Original languageEnglish
Pages (from-to)698-710
Number of pages13
JournalJournal of Applied Microbiology
Volume101
Issue number3
DOIs
Publication statusPublished - Sep 2006
Externally publishedYes

Fingerprint

Bioelectric Energy Sources
microbial fuel cells
fuel cells
Bacterial Structures
Biofilms
bacterial communities
biofilm
community structure
RNA libraries
Gammaproteobacteria
Electrodes
gamma-Proteobacteria
Gene Library
rRNA Genes
electrodes
Enterobacter
Denaturing Gradient Gel Electrophoresis
Klebsiella
denaturing gradient gel electrophoresis
Enterobacteriaceae

Keywords

  • 16S rRNA
  • Anaerobic respiration
  • Biofilm
  • Denaturing gradient gel electrophoresis
  • Electrochemical activity
  • Fe(III)-reducing bacteria
  • Klebsiella oxytoca
  • Microbial fuel cell

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Microbiology

Cite this

Kim, G. T., Webster, G., Wimpenny, J. W. T., Kim, B. H., Kim, H. J., & Weightman, A. J. (2006). Bacterial community structure, compartmentalization and activity in a microbial fuel cell. Journal of Applied Microbiology, 101(3), 698-710. https://doi.org/10.1111/j.1365-2672.2006.02923.x

Bacterial community structure, compartmentalization and activity in a microbial fuel cell. / Kim, G. T.; Webster, G.; Wimpenny, J. W T; Kim, B. H.; Kim, H. J.; Weightman, A. J.

In: Journal of Applied Microbiology, Vol. 101, No. 3, 09.2006, p. 698-710.

Research output: Contribution to journalArticle

Kim, G. T. ; Webster, G. ; Wimpenny, J. W T ; Kim, B. H. ; Kim, H. J. ; Weightman, A. J. / Bacterial community structure, compartmentalization and activity in a microbial fuel cell. In: Journal of Applied Microbiology. 2006 ; Vol. 101, No. 3. pp. 698-710.
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