Bacterial cellulose/acrylamide pH-sensitive smart hydrogel: Development, characterization, and toxicity studies in ICR mice model

Manisha Pandey, Najwa Mohamad, Mohd Cairul Iqbal Mohd Amin

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The objective of this study is to synthesize and evaluate acute toxicity of the bacterial cellulose (BC)/acrylamide (Am) hydrogels as noncytotoxic and biocompatible oral drug delivery vehicles. A novel series of solubilized BC/Am hydrogels were synthesized using a microwave irradiation method. The hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), swelling ratio, porosity, drug release, and in vitro and in vivo biocompatibility experiments. FTIR spectra revealed that the BC crystallinity and gel fraction decreased as the NaOH concentration increased from 2% to 10% w/v, whereas the optical transparency, pH sensitivity, and porosity were enhanced with increasing alkali concentration. Theophylline was used as a model drug for drug loading and release studies. The percentage of drug released was higher at pH 7.4 compared to pH 1.5. In vitro cytotoxicity and hemolytic tests indicated that the BC/Am hydrogel is noncytotoxic and hemocompatible. Results of acute oral toxicity tests on ICR mice suggested that the hydrogels are nontoxic up to 2000 mg/kg when administered orally, as no toxic response or histopathological changes were observed in comparison to control mice. The results of this study demonstrated that the pH-sensitive smart hydrogel makes it a possible safe carrier for oral drug delivery.

Original languageEnglish
Pages (from-to)3596-3608
Number of pages13
JournalMolecular Pharmaceutics
Volume11
Issue number10
DOIs
Publication statusPublished - 6 Oct 2014

Fingerprint

Inbred ICR Mouse
Hydrogels
Acrylamide
Hydrogel
Cellulose
Porosity
Fourier Transform Infrared Spectroscopy
Acute Toxicity Tests
Pharmaceutical Preparations
Drug Carriers
Poisons
Alkalies
Theophylline
Microwaves
Gels
In Vitro Techniques
Drug Liberation

Keywords

  • acrylamide
  • acute oral toxicity
  • bacterial cellulose
  • cytotoxicity
  • hydrogel
  • microwave irradiation

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Molecular Medicine
  • Drug Discovery

Cite this

Bacterial cellulose/acrylamide pH-sensitive smart hydrogel : Development, characterization, and toxicity studies in ICR mice model. / Pandey, Manisha; Mohamad, Najwa; Mohd Amin, Mohd Cairul Iqbal.

In: Molecular Pharmaceutics, Vol. 11, No. 10, 06.10.2014, p. 3596-3608.

Research output: Contribution to journalArticle

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