Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model

Mohd Heikal Mohd Yunus, B. S. Aminuddin, J. Jeevanan, H. C. Chen, S. H. Sharifah, Ruszymah Idrus

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The objective of this study was to regenerate the tracheal epithelium using autologous nasal respiratory epithelial cells in a sheep model. Respiratory epithelium and fibroblast cells were harvested from nasal turbinates and cultured for 1 week. After confluence, respiratory epithelium and fibroblast cells were suspended in autologous fibrin polymerized separately to form a tissue-engineered respiratory epithelial construct (TEREC). A 3 × 2 cm2 tracheal mucosal defect was created, and implanted with TEREC and titanium mesh as a temporary scaffold. The control groups were divided into 2 groups: polymerized autologous fibrin devoid of cells (group 1), and no construct implanted (group 2). All sheep were euthanized at 4 weeks of implantation. Gross observation of the trachea showed minimal luminal stenosis formation in the experimental group compared to the control groups. Macroscopic evaluation revealed significant mucosal fibrosis in control group 1 (71.8%) as compared to the experimental group (7%). Hematoxylin and eosin staining revealed the presence of minimal overgrowth of fibrous connective tissue covered by respiratory epithelium. A positive red fluorescence staining of PKH26 on engineered tissue 4 weeks after implantation confirmed the presence of cultured nasal respiratory epithelial cells intercalated with native tracheal epithelial cells. Scanning electron microscopy showed the presence of short microvilli representing immature cilia on the surface of the epithelium. Our study showed that TEREC was a good replacement for a tracheal mucosal defect and was able to promote natural regenesis of the tracheal epithelium with minimal fibrosis. This study highlighted a new technique in the treatment of tracheal stenosis.

Original languageEnglish
Pages (from-to)292-302
Number of pages11
JournalCells Tissues Organs
Volume192
Issue number5
DOIs
Publication statusPublished - Oct 2010

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Respiratory Mucosa
Sheep
Nose
Epithelium
Epithelial Cells
Fibrin
Control Groups
Fibrosis
Fibroblasts
Staining and Labeling
Tracheal Stenosis
Turbinates
Cilia
Hematoxylin
Eosine Yellowish-(YS)
Microvilli
Trachea
Titanium
Connective Tissue
Electron Scanning Microscopy

Keywords

  • Fibrin, autologous
  • Respiratory epithelium
  • Tissue engineering

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Autologous implantation of bilayered tissue-engineered respiratory epithelium for tracheal mucosal regenesis in a sheep model. / Mohd Yunus, Mohd Heikal; Aminuddin, B. S.; Jeevanan, J.; Chen, H. C.; Sharifah, S. H.; Idrus, Ruszymah.

In: Cells Tissues Organs, Vol. 192, No. 5, 10.2010, p. 292-302.

Research output: Contribution to journalArticle

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