Autogenic feeder free system from differentiated mesenchymal progenitor cells, maintains pluripotency of the MEL-1 human embryonic stem cells

Tze Sean Khoo, Noor Hamidah Hussin, Sue Mian Then, A. Rahman A. Jamal

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Human embryonic stem cells (hESc) are known for its pluripotency and self renewal capability, thus possess great potential in regenerative medicine. However, the lack of suitable xenofree extracellular matrix substrate inhibits further applications or the use of hESc in cell-based therapy. In this study, we described a new differentiation method, which generates a homogeneous population of mesenchymal progenitor cells (hESc-MPC) from hESc via epithelial-mesenchymal transition. The extracellular matrix (ECM) proteins from hESc-MPC had in turn supported the undifferentiated expansion of hESc. Immunocytochemistry and flow cytometry characterization of hESc-MPC revealed the presence of early mesenchymal markers. Tandem mass spectometry analysis of ECM produced by hESc-MPC revealed the presence of a mixture of extracellular proteins which includes tenascin C, fibronectin, and vitronectin. The pluripotency of hESc (MEL-1) cultured on the ECM was maintained as shown by the expression of pluripotent genes (FoxD3, Oct-4, Tdgf1, Sox-2, Nanog, hTERT, Rex1), protein markers (SSEA-3, SSEA-4, TRA-1-81, TRA-1-60, Oct-4.) and the ability to differentiate into cells representative of ectoderm, endoderm and mesoderm. In summary, we have established a xeno-free autogenic feeder free system to support undifferentiated expansion of hESc, which could be of clinical relevance.

Original languageEnglish
Pages (from-to)110-118
Number of pages9
JournalDifferentiation
Volume85
Issue number3
DOIs
Publication statusPublished - Feb 2013

Fingerprint

Mesenchymal Stromal Cells
Extracellular Matrix
Human Embryonic Stem Cells
Vitronectin
Tenascin
Endoderm
Ectoderm
Regenerative Medicine
Epithelial-Mesenchymal Transition
Extracellular Matrix Proteins
Mesoderm
Cell- and Tissue-Based Therapy
Fibronectins
Flow Cytometry
Proteins
Immunohistochemistry
Gene Expression

Keywords

  • Autogenic
  • Differentiation
  • Embryonic stem cell
  • Feeder free
  • Mesenchymal progenitor cell

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology
  • Cancer Research

Cite this

Autogenic feeder free system from differentiated mesenchymal progenitor cells, maintains pluripotency of the MEL-1 human embryonic stem cells. / Khoo, Tze Sean; Hussin, Noor Hamidah; Then, Sue Mian; A. Jamal, A. Rahman.

In: Differentiation, Vol. 85, No. 3, 02.2013, p. 110-118.

Research output: Contribution to journalArticle

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