Augmentation of angiogenic and endothelial associated gene expression by EDM50 in human chorion-derived stem cells.

N. F Mohd Manzor, Chua Kien Hui, Geok Chin Tan, A. E. Tan, H. Abdul Rahman

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The objective of this study was to investigate the angiogenic potential of human chorion-derived stem cells (CDSC) cultured in medium containing bFGF and VEGF (EDM50). Total RNA was extracted from cells cultured in FD+10% FBS and EDM50. Quantitative RT-PCR was carried out to score the differential mRNA expression of genes involve in angiogenesis and endothelial differentiation. Our finding demonstrated that all angiogenic and endothelial associated genes were expressed higher in EDM50. Expression level of ANG-1, eNOS and VEGFR2 were significantly higher in EDM50 compared to FD+10% FBS. Our results suggested that human CDSC cultured in EDM50 can be used for angiogenesis purpose in regenerative medicine.

Original languageEnglish
Pages (from-to)11-12
Number of pages2
JournalThe Medical journal of Malaysia
Volume63 Suppl A
Publication statusPublished - Jul 2008

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Chorion
Stem Cells
Gene Expression
Regenerative Medicine
Vascular Endothelial Growth Factor A
Cultured Cells
RNA
Polymerase Chain Reaction
Messenger RNA
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Augmentation of angiogenic and endothelial associated gene expression by EDM50 in human chorion-derived stem cells. / Manzor, N. F Mohd; Kien Hui, Chua; Tan, Geok Chin; Tan, A. E.; Abdul Rahman, H.

In: The Medical journal of Malaysia, Vol. 63 Suppl A, 07.2008, p. 11-12.

Research output: Contribution to journalArticle

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AU - Abdul Rahman, H.

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