Attachment, proliferation, and morphological properties of human dermal fibroblasts on ovine tendon collagen scaffolds

A comparative study

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Introduction: Collagen type I is widely used as a biomaterial for tissue-engineered substitutes. This study aimed to fabricate different three-dimensional (3D) scaffolds using ovine tendon collagen type I (OTC-I), and compare the attachment, proliferation and morphological features of human dermal fibroblasts (HDF) on the scaffolds. Methods: This study was conducted between the years 2014 to 2016 at the Tissue Engineering Centre, UKM Medical Centre. OTC-I was extracted from ovine tendon, and fabricated into 3D scaffolds in the form of sponge, hydrogel and film. A polystyrene surface coated with OTC-I was used as the 2D culture condition. Genipin was used to crosslink the OTC-I. A non-coated polystyrene surface was used as a control. The mechanical strength of OTC-I scaffolds was evaluated. Attachment, proliferation and morphological features of HDF were assessed and compared between conditions. Results: The mechanical strength of OTC-I sponge was significantly higher than that of the other scaffolds. OTC-I scaffolds and the coated surface significantly enhanced HDF attachment and proliferation compared to the control, but no differences were observed between the scaffolds and coated surface. In contrast, the morphological features of HDF including spreading, filopodia, lamellipodia and actin cytoskeletal formation differed between conditions. Conclusion: OTC-I can be moulded into various scaffolds that are biocompatible and thus could be suitable as scaffolds for developing tissue substitutes for clinical applications and in vitro tissue models. However, further study is required to determine the effect of morphological properties on the functional and molecular properties of HDF.

Original languageEnglish
Pages (from-to)33-43
Number of pages11
JournalMalaysian Journal of Medical Sciences
Volume24
Issue number2
DOIs
Publication statusPublished - 2017

Fingerprint

Collagen Type I
Tendons
Sheep
Collagen
Fibroblasts
Skin
Pseudopodia
Polystyrenes
Porifera
Tissue Scaffolds
Biocompatible Materials
Tissue Engineering
Actins

Keywords

  • Collagen type I
  • Fibroblasts
  • Hydrogel
  • Proliferation
  • Tissue engineering

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{0489b50006e14a8b9549616e48f65a6f,
title = "Attachment, proliferation, and morphological properties of human dermal fibroblasts on ovine tendon collagen scaffolds: A comparative study",
abstract = "Introduction: Collagen type I is widely used as a biomaterial for tissue-engineered substitutes. This study aimed to fabricate different three-dimensional (3D) scaffolds using ovine tendon collagen type I (OTC-I), and compare the attachment, proliferation and morphological features of human dermal fibroblasts (HDF) on the scaffolds. Methods: This study was conducted between the years 2014 to 2016 at the Tissue Engineering Centre, UKM Medical Centre. OTC-I was extracted from ovine tendon, and fabricated into 3D scaffolds in the form of sponge, hydrogel and film. A polystyrene surface coated with OTC-I was used as the 2D culture condition. Genipin was used to crosslink the OTC-I. A non-coated polystyrene surface was used as a control. The mechanical strength of OTC-I scaffolds was evaluated. Attachment, proliferation and morphological features of HDF were assessed and compared between conditions. Results: The mechanical strength of OTC-I sponge was significantly higher than that of the other scaffolds. OTC-I scaffolds and the coated surface significantly enhanced HDF attachment and proliferation compared to the control, but no differences were observed between the scaffolds and coated surface. In contrast, the morphological features of HDF including spreading, filopodia, lamellipodia and actin cytoskeletal formation differed between conditions. Conclusion: OTC-I can be moulded into various scaffolds that are biocompatible and thus could be suitable as scaffolds for developing tissue substitutes for clinical applications and in vitro tissue models. However, further study is required to determine the effect of morphological properties on the functional and molecular properties of HDF.",
keywords = "Collagen type I, Fibroblasts, Hydrogel, Proliferation, Tissue engineering",
author = "Fauzi, {Mh Busra} and Yogeswaran Lokanathan and Nadzir, {Masrina Mohd} and Saim Aminuddin and Ruszymah, {Bt Hj Idrus} and Chowdhury, {Shiplu Roy}",
year = "2017",
doi = "10.21315/mjms2017.24.2.5",
language = "English",
volume = "24",
pages = "33--43",
journal = "Malaysian Journal of Medical Sciences",
issn = "1394-195X",
publisher = "Universiti Sains Malaysia",
number = "2",

}

TY - JOUR

T1 - Attachment, proliferation, and morphological properties of human dermal fibroblasts on ovine tendon collagen scaffolds

T2 - A comparative study

AU - Fauzi, Mh Busra

AU - Lokanathan, Yogeswaran

AU - Nadzir, Masrina Mohd

AU - Aminuddin, Saim

AU - Ruszymah, Bt Hj Idrus

AU - Chowdhury, Shiplu Roy

PY - 2017

Y1 - 2017

N2 - Introduction: Collagen type I is widely used as a biomaterial for tissue-engineered substitutes. This study aimed to fabricate different three-dimensional (3D) scaffolds using ovine tendon collagen type I (OTC-I), and compare the attachment, proliferation and morphological features of human dermal fibroblasts (HDF) on the scaffolds. Methods: This study was conducted between the years 2014 to 2016 at the Tissue Engineering Centre, UKM Medical Centre. OTC-I was extracted from ovine tendon, and fabricated into 3D scaffolds in the form of sponge, hydrogel and film. A polystyrene surface coated with OTC-I was used as the 2D culture condition. Genipin was used to crosslink the OTC-I. A non-coated polystyrene surface was used as a control. The mechanical strength of OTC-I scaffolds was evaluated. Attachment, proliferation and morphological features of HDF were assessed and compared between conditions. Results: The mechanical strength of OTC-I sponge was significantly higher than that of the other scaffolds. OTC-I scaffolds and the coated surface significantly enhanced HDF attachment and proliferation compared to the control, but no differences were observed between the scaffolds and coated surface. In contrast, the morphological features of HDF including spreading, filopodia, lamellipodia and actin cytoskeletal formation differed between conditions. Conclusion: OTC-I can be moulded into various scaffolds that are biocompatible and thus could be suitable as scaffolds for developing tissue substitutes for clinical applications and in vitro tissue models. However, further study is required to determine the effect of morphological properties on the functional and molecular properties of HDF.

AB - Introduction: Collagen type I is widely used as a biomaterial for tissue-engineered substitutes. This study aimed to fabricate different three-dimensional (3D) scaffolds using ovine tendon collagen type I (OTC-I), and compare the attachment, proliferation and morphological features of human dermal fibroblasts (HDF) on the scaffolds. Methods: This study was conducted between the years 2014 to 2016 at the Tissue Engineering Centre, UKM Medical Centre. OTC-I was extracted from ovine tendon, and fabricated into 3D scaffolds in the form of sponge, hydrogel and film. A polystyrene surface coated with OTC-I was used as the 2D culture condition. Genipin was used to crosslink the OTC-I. A non-coated polystyrene surface was used as a control. The mechanical strength of OTC-I scaffolds was evaluated. Attachment, proliferation and morphological features of HDF were assessed and compared between conditions. Results: The mechanical strength of OTC-I sponge was significantly higher than that of the other scaffolds. OTC-I scaffolds and the coated surface significantly enhanced HDF attachment and proliferation compared to the control, but no differences were observed between the scaffolds and coated surface. In contrast, the morphological features of HDF including spreading, filopodia, lamellipodia and actin cytoskeletal formation differed between conditions. Conclusion: OTC-I can be moulded into various scaffolds that are biocompatible and thus could be suitable as scaffolds for developing tissue substitutes for clinical applications and in vitro tissue models. However, further study is required to determine the effect of morphological properties on the functional and molecular properties of HDF.

KW - Collagen type I

KW - Fibroblasts

KW - Hydrogel

KW - Proliferation

KW - Tissue engineering

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U2 - 10.21315/mjms2017.24.2.5

DO - 10.21315/mjms2017.24.2.5

M3 - Article

VL - 24

SP - 33

EP - 43

JO - Malaysian Journal of Medical Sciences

JF - Malaysian Journal of Medical Sciences

SN - 1394-195X

IS - 2

ER -